Hydrogen-Rich Saline Ameliorates Allergic Rhinitis by Reversing the Imbalance of Th1/Th2 and Up-Regulation of CD4+CD25+Foxp3+Regulatory T Cells, Interleukin-10, and Membrane-Bound Transforming Growth Factor-β in Guinea Pigs.

It is well known that CD4+CD25+Foxp3+Treg cells play an important role in the development of allergic rhinitis (AR); the defect of cell numbers and functions contribute to AR. Hydrogen has been proven effective in alleviating symptoms of AR. We herein aim to verify the protective effects of hydrogen on CD4+CD25+Foxp3+Treg cells in guinea pigs with AR and to explore the effect of hydrogen-rich saline (HRS) on CD4+CD25+Foxp3+Treg cells in animals with AR and investigate the underlying anti-inflammatory mechanism.

Hydrogen-rich saline attenuates eosinophil activation in a guinea pig model of allergic rhinitis via reducing oxidative stress.

Background: It is well considered that reactive oxygen species (ROS) plays a prominent causative role in the development of allergic rhinitis (AR), and eosinophils cells as important allergic inflammatory cells contribute to elevating oxidative stress. Hydrogen, emerging as a novel antioxidant, has been proven effective in selectively reducing ROS in animals models of oxidative damage. We herein aim to verify protective effects of hydrogen on eosinophils cells in guinea pigs models of AR.

Identification and Characterization of CD133(pos) Subpopulation Cells From a Human Laryngeal Cancer Cell Line.

Background: Recent research indicates that CD133 are expressed in several kinds of stem cells, among which, its high expression in laryngeal carcinoma has caused wide concern. To further explore efficaciously targeting drugs to laryngeal carcinoma stem cells (CSCs), we transplanted a solid tumor from CSCs into abdominal subcutaneous tissue of nude mice, and then compared the biological characteristics of laryngeal solid tumors with or without cisplatin intervention.

Material/methods: In this study, the expression of CD133 was detected in the Hep-2 cell line by flow cytometry.

[Clinical analysis of simple orbital blowout fracture].

Objective: To discuss the clinical features and treatment methods for simple orbital blowout fracture.

Method: Retrospective analysis of the CT images of 16 orbital blowout fracture case, and identification of the sites, degree, patterns and features of fractures. Among the 16 cases, 2 cases adopted conservative treatment; 11 cases gained a reduction of orbital fracture through endoscopic transnasal surgery; the other 3 patients choosed endoscopic transnasal surgery and Caldwell-Luc operations.

[Twenty-six clinical case analysis of defect repairing after the resection of basal cell carcinoma on external nose].

Objective: To study a surgical and repairing method for defects after the resection of basal cell carcinoma of external nose.

Method: There are 26 cases of basal cell carcinoma that tumors have been resected completely after operation. For defect repairing in those 26 cases, 2 cases adopt direct suture method; 2 cases use skin graft repairing methods; 18 cases employ naselabial skin flap repairing method; 4 cases choose forehead pedicle skin flap repairing methods.

Impact of carbon monoxide/heme oxygenase on hydrogen sulfide/cystathionine-γ-lyase pathway in the pathogenesis of allergic rhinitis in guinea pigs.

Objective: The discovery of carbon monoxide (CO) and hydrogen sulfide (H2S) as pathogenic signaling molecules in airway-related diseases has led to significant insights into the pathophysiologic mechanisms underlying the development of allergic rhinitis (AR). The potential crosstalk between CO and H2S signaling pathways in AR has not been adequately investigated. This study was performed to elucidate the mechanistic relationship between CO and H2S in AR.

[The effect of dendritic cells on allergic rhinitis in sublingual therapy].

Dendritic cells (DCs) is known as the most potential and professional antigen presenting cells (APC), it mainly involves in the cellular immunity and T cell dependent humoral immunity, which plays a key role in the immune response and is one of the most hot areas in immunology in recent years. DCs plays a key role in allergic rhinitis (AR) and is one of the most important mechanism of AR treating by sublingual immunotherapy (SLIT). This article reviewed the mechanism of the role of DCs in AR and AR treating by SLIT.

[A case report of vagus nerve invasion with disseminated herpes zoster].

A case of vagus nerve invasion with disseminated herpes zoster was reported. Clinical manifestation of disseminated herpes zoster and vagus nerve injury. relevant imaging examination and laboratory examination can help to establish a preliminary diagnosis.

Cervical necrotizing fasciitis and a descending mediastinal abscess caused by acute epiglottitis with diabetes mellitus: a life-threatening complication.

Cervical necrotizing fasciitis and a descending mediastinal abscess of the neck following acute epiglottitis, life-threatening complications, is reported in a 43-year-old man with DM. The bacterial culture showed Peptostreptococcus anaerobius. The patient recovered after surgical debridement and broad-spectrum antibiotics therapy.

[The expression and relationship of CXCR4 and microvessel density in laryngeal squamous cell carcinoma].

Objective: To investigate the expression of chemokine receptor 4 (CXCR4) in laryngeal squamous carcinoma and its correlation with clinicopathology character and the building of microvessel density (MVD). To evaluate its role in the carcinogenesis and progression in laryngeal squamous carcinoma.

Method: The expression of CXCR4 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 42 patients were examined by RT-PCR, immunohistochemical staining and Image-pro-plus software.

[The expression of cyclooxygenase-2 in laryngeal squamous cell carcinoma and its relationship with MVD].

Objective: To investigate the expression of cyclooxygenase-2 in laryngeal squamous cell carcinoma and the correlation with tumor angiogenesis.

Method: The COX-2 and FVIII of 42 cases of laryngeal squamous cell carcinoma were detected by immunohistochemical SABC method,and the correlation between the expression rates, clinical significance and microvessel density(MVD) was analyzed.

Result: The positive rate of COX-2 in laryngeal squamous cell carcinoma tissues and adjacent normal tissues were 71.

[The expression of VEGF and iNOS in laryngeal squamous cell carcinoma and its clinical significance].

Objective: To investigate the mRNA expression of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) in laryngeal squamous carcinoma and its correlation with clinicopathologic parameters, to identify the interactions between the expression of VEGF and iNOS.

Method: The mRNA expression of VEGF and iNOS in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 40 patients were examined by reverse transcription-polymerase chain reaction.

Result: The positive expression rate of VEGF and iNOS in tumor samples were significantly higher than that in normal tissues (P < 0.

[Expression of VEGF and VEGF-C in laryngeal carcinoma and their clinical significance].

Objective: To study the gene expression of Vascular Endothelial Growth Factor (VEGF) and Vascular Endothelial Growth Factor C (VEGF-C) in laryngeal carcinoma and their relation with clinic pathologic features.

Method: VEGF and VEGF C gene expression in 20 cases of fresh laryngeal carcinoma, cutting edge and normal tissue were examined by reverse transcription polymerase chain reaction (RT-PCR).

Result: The gene expression of VEGF and VEGF-C is different among laryngeal carcinoma, cutting edge and normal tissue in the same patient.