Development of rice water-saving and drought resistance quantitative evaluation system of wide water ecological range based on quantitative gradient water control.

The drought resistance of rice is an indirect observational and complex trait whose phenotype is reflected in the response of directly observational traits to drought stress. To objectively and accurately evaluate the drought resistance of rice, soil moisture gradient quantification was designed as a general water index among different soil types. Through soil water control, water consumption calculation, yield test, trait examination, and statistical analysis, the relationship between quantitative water control treatment and rice yield drought resistance was studied to establish a quantitative and controllable evaluation system of rice drought resistance.

[Screening for virulence strains of Metarhizium against Dorysthenes hydropicus pascoes].

Objective: The aim of the present study was to screen the Metarhizium strains with high virulence against the larvae of Dorysthenes hydropicus, a serious pest of Citrus grandis.

Method: Thirty six strains of Metarhiziums were isolated from the soil of C. grandis GAP base and collected from other institutions, and the pathogenicity of these strains against 1st instar larvae of D.

[Isolation and identification of Metarhizium from Citrus grandis 'tomentosa' GAP base].

A type of entomopathogenic fungus of soil in Citrus grandis 'tomentosa' production base was isolated and identified with morphological and molecular biological methods, including pathogenesis, spore characteristic and ITS sequence analysis were conducted. The results showed that eighteen entomopathogenic fungi strains were isolated from the Tenebrio molitor infected in the soil samples, which were identified as Metarhizium anisopliae var. anisopliae.

[Lethal effects of entomopathogenic nematodes on larvae of Dorysthenes hydropicus in laboratory experiment].

In order to explore the environmental pest management method of Dorysthenes hydropicus, three strains of entomopathogenic nematodes, viz. Heterorhabditis bacteriphora (H06), Steinernema scapterisci (SS), S. carpocapsae (All) were used on larvae of Dorysthenes hydropicus, with treatments of 0, 5 000 and 10 000 nematodes each larva.