Identification and validation of a T cell marker gene-based signature to predict prognosis and immunotherapy response in gastric cancer.

Although the role of T cells in tumor immunity and modulation of the tumor microenvironment (TME) has been extensively studied, their precise involvement in gastric adenocarcinoma remains inadequately explored. In this work, we analyzed the single-cell RNA sequencing data set in GSE183904 and identified 322 T cell marker genes using the "FindAllMarkers" method of the R package "Seurat". STAD patients in the TCGA database were divided into high-risk and low-risk categories based on risk scores.

Analysis of spatial-temporal dynamic distribution and related factors of tuberculosis in China from 2008 to 2018.

Through spatial-temporal scanning statistics, the spatial-temporal dynamic distribution of pulmonary tuberculosis incidence in 31 provinces and autonomous regions of China from 2008 to 2018 is obtained, and the related factors of spatial-temporal aggregation of tuberculosis in China are analyzed to provide strong scientific basis and data support for the prevention and control of pulmonary tuberculosis. This is a retrospective study, using spatial epidemiological methods to reveal the spatial-temporal clustering distribution characteristics of China's tuberculosis epidemic from 2008 to 2018, in which cases data comes from the China Center for Disease Control and prevention. Office Excel is used for general statistical description, and the single factor correlation analysis adopts χ Test (or trend χ Inspection).

Reverse relationship between autophagy and apoptosis in an in vitro model of cortical neuronal injury.

Autophagy and apoptosis are intertwined, and their relationship involves complex cross-talk. Whether the activation and inhibition of autophagy protect or damage neurons in the central nervous system has been a matter of longstanding controversy. We investigated the effect of autophagy on the apoptosis of cortical neurons after oxygen- and glucose-deprivation/reoxygenation (OGD/R) injury in vitro and found that protective mechanism activation was the predominant response to enhanced autophagy activation and increased autophagic flux.

Neuritin attenuates oxygen-glucose deprivation/reoxygenation (OGD/R)-induced neuronal injury by promoting autophagic flux.

The autophagy/apoptosis interaction has always been a focus of study in pathogenicity models. Neuritin is a neurotrophic factor that is highly expressed primarily in the central nervous system. Our previous study revealed that it protects against apoptosis in cortical neurons subjected to oxygen-glucose deprivation (OGD)/reoxygenation (OGD/R), and later animal experiments revealed that it can increase the expression of the autophagy-related protein LC3.