Design, Synthesis, and Evaluation of Novel Thiazole-Containing Algicides Inspired by Bacillamide A.

The pursuit of highly effective, low-toxicity, and eco-friendly algicides for controlling and eradicating harmful algal blooms (HABs) is of paramount importance. The natural allelochemical bacillamide A has displayed impressive algicidal activity against harmful algae with favorable safety profiles. However, the poor synthetic efficiency and large dose requirements of bacillamide A limit its further application.

Inhibitory effect and mechanism of algicidal bacteria on Chaetomorpha valida.

Chaetomorpha valida, filamentous green tide algae, poses a significant threat to both aquatic ecosystems and aquaculture. Vibrio alginolyticus Y20 is a new algicidal bacterium with an algicidal effect on C. valida.

An overview on recent advances of reversible fluorescent probes and their biological applications.

Due to the simplicity and low detection limit, fluorescent probes are widely used in both analytical sensing and optical imaging. Compared to conventional fluorescent probes, reversibility endows the reversible fluorescent probe outstanding advantages and special properties, making reversible fluorescent probes with capable of quantitative, repetitive or circulatory. Reversible fluorescent probes can also monitor the concentration dynamics of target analytes in real time, such as metal ions, proteins and enzymes, as well as intracellular redox processes, which have been widely applied in various fields.

Functional characterization of secologanin synthase-like homologous genes suggests their involvement in the biosynthesis of diverse metabolites in the secoiridoid biosynthetic pathway of Camptotheca acuminata Decne.

The assignment of functions based on homology has recently been challenged by the frequent discovery of functional divergence among homologous gene family members of enzymes involved in plant secondary metabolism. Secologanin synthase (SLS) is the key CYP450 enzyme that acts critically in the biosynthesis of Strychnos alkaloid scaffold. In this study, to fully elucidate the mechanism that underlies metabolic variation, the CYP450 paralogs that participate in oxidative transformation of the secoiridoid pathway were functionally characterized by combining multitiered strategies of metabolite profiling, phylogenetic analyses, biochemistry assays and reverse genetics techniques.

Citral-loaded chitosan/carboxymethyl cellulose copolymer hydrogel microspheres with improved antimicrobial effects for plant protection.

The extensive use of chemical pesticides in agricultural production has caused great damage to the soil and ecological environment. Citral, a monoterpene aldehyde, has been shown to inhibit the growth of a variety of pathogenic fungi by affecting mitochondrial structure. However, the high volatility and chemical instability of citral may restrict its applications in the agricultural industries.

Microplastics profile in a typical urban river in Beijing.

Municipal wastewater treatment plants (WWTPs) are known to be important point sources of microplastic pollution in the environment because they discharge large volumes of microplastic-containing effluents into nearby rivers. However, the fate of these microplastics once they enter the urban rivers is not well understood. The present study focused on the Qing River, a typical urban river in Beijing that receives effluents from four nearby WWTPs.

Microplastics in sediments from an interconnected river-estuary region.

Microplastics are an emerging pollutant of international concern due to its wide distribution through various pathways. Estuary is an important pathway for land microplastics to enter into the oceans by rivers. In this study, we hypothesized that microplastics would sink into estuary sediment during diffusion and transmission from river before entering into the sea, which results in higher accumulation of microplastics in proximity to river-estuary than in the oceans.

Effects of nitrogen source and N/P on growth and photosynthesis in the invasive marine macroalga Chaetomorpha valida.

The invasive marine macroalga Chaetomorpha valida blooms frequently in Apostichopus japonicus culture ponds in North China, resulting in negative environmental consequences. The factors driving this algal overgrowth are unclear. Previous studies observed that eutrophication strongly influences abnormal growth of nuisance macrophytes, but relatively few studies have addressed the types and abundance of nitrogen in A.

Two classes of cytochrome P450 reductase genes and their divergent functions in Camptotheca acuminata Decne.

Cytochrome P450 monooxygenases (CYP450s) and their auxiliary cytochrome P450 reductases (CPRs) are important and commonly involved in the biosynthesis of camptothecin (CPT). To better understand the possible functions of CPRs in planta, we first isolated two CaCPRs genes from Camptotheca acuminata. Sequence analysis revealed the presence of common conserved FMN-, FAD- and NADPH-binding motifs in putative CaCPR1/CaCPR2 proteins.

Gene identification and antimicrobial activity analysis of a novel lysozyme from razor clam Sinonovacula constricta.

Lysozymes are important immune effectors present in phylogenetically diverse organisms. They play vital roles in bacterial elimination during early immune responses. In the present study, a second invertebrate-type (i-type) lysozyme gene from razor clam Sinonovacula constricta (denoted as ScLYZ-2) was cloned by RACE and nested PCR methods.

Factors that affect the growth and photosynthesis of the filamentous green algae, , in static sea cucumber aquaculture ponds with high salinity and high pH.

, dominant filamentous green algae, can be harmful to sea cucumber growth in aquaculture ponds of China. In order to understand the environmental factors affecting the growth of in sea cucumber aquaculture ecosystems, a combination of field investigations and laboratory experiments were conducted. Field surveys over one year revealed that survived in sea cucumber aquaculture ponds in salinities ranging from 24.

β-Integrin mediates LPS-induced coelomocyte apoptosis in sea cucumber Apostichopus japonicus via the integrin/FAK/caspase-3 signaling pathway.

Lipopolysaccharides (LPS) can induce the apoptosis of coelomocytes in Apostichopus japonicus (A. japonicus), and β-integrin serves as an apoptotic inhibitor during this process. However, the underlying mechanism in invertebrates is largely unknown.

Functional characterization of two ABC transporters in Sinonovacula constricta gills and their barrier action in response to pathogen infection.

P-glycoprotein (P-gp or ABCB1) and multidrug resistance associated proteins (MRPs or ABCC) belonging to the family of ATP-binding cassette (ABC) transporters protect aquatic organisms from various toxicants and pathogen exposure. The function of ABC transporters of Sinonovacula constricta in response to pathogens was explored by cloning the complete cDNA of ABCB1 and ABCC5 of S. constricta through the rapid amplification of cDNA ends.

Cloning, expression analysis and functional characterization of an interleukin-1 receptor-associated kinase 4 from Apostichopus japonicus.

Interleukin 1 receptor-associated kinase 4 (IRAK4) is a key factor in TLR-mediated host immune function. In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 1311 bp encoding a 436-amino-acid (aa) residue polyprotein with the typical death domain (10-113aa) and the kinase domain (160-426aa).

Identification and characterization of a novel PRR of fibrinogen-related protein in Apostichopus japonicus.

Fibrinogen-related proteins (FREPs) play important roles in innate immunity by recognizing pathogen associated molecular patterns on pathogenic bacteria surfaces via conserved fibrinogen-like domain (FBG). In this paper, the full-length cDNA of Apostichopus japonicus FREP (designated as AjFREP) was cloned combined with rapid amplification of cDNA ends (RACE) and transcriptome sequencing. The full-length cDNA of AjFREP was of 2110 bp with an open reading frame (ORF) of 1659 bp.

Divergent immune roles of two fucolectin isoforms in Apostichopus japonicus.

The F-type lectin (fucolectin) family represents a new group with innate immunity. In this study, two fucolectin isoforms (designated as AjFTL-1 and AjFTL-2) were identified in sea cucumber (Apostichopus japonicus) through rapid amplification of cDNA ends. Full-length cDNAs of AjFTL-1 and AjFTL-2 measured 2134 and 1286 bp, encoding two secreted proteins comprising 317 and 181 amino acid residues, respectively.

Analysis of gut microbiota revealed Lactococcus garviaeae could be an indicative of skin ulceration syndrome in farmed sea cucumber Apostichopus japonicus.

Accumulative evidence has supported the pivotal roles of gut microbiota in shaping host health in a wide range of animals. However, the relationship between gut microbiota and sea cucumber disease is poorly understood. Using the Illumina sequencing of bacterial 16 S rRNA gene, we investigated the divergence of gut bacterial communities between healthy and skin ulceration syndrome (SUS) diseased Apostichopus japonicus.

[Analysis of eleven mineral elements for eight benthic marine diatoms using AAS].

Eleven mineral-elements, Mg, Fe, K, Ca, Mn, Zn, Cu, Ni, Pb, Cr and Cd, for eight benthic marine diatoms were determined by using flame atomic absorption spectrophotometry (AAS). This method is rapid with high precision and accuracy. The relative standard deviation, correlative coefficient and recovery rate are less then 6.