Age-related decline in CD8 tissue resident memory T cells compromises antitumor immunity.

Aging compromises antitumor immunity, but the underlying mechanisms remain elusive. Here, we report that aging impairs the generation of CD8 tissue resident memory T (T) cells in nonlymphoid tissues in mice, thus compromising the antitumor activity of aged CD8 T cells, which we also observed in human lung adenocarcinoma. We further identified that the apoptosis regulator BFAR was highly enriched in aged CD8 T cells, in which BFAR suppressed cytokine-induced JAK2 signaling by activating JAK2 deubiquitination, thereby limiting downstream STAT1-mediated T reprogramming.

Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysis.

Here, we present a protocol for exploring the effects of PPP1R15A inhibitor, Sephin1, on antitumor immunity of B16F1 subcutaneous tumor in mice. We describe steps for constructing single-cell transcriptome and TCR libraries, sequencing, and using sequencing data for the integration of expression and TCR data. We then detail procedures for gene differentiation, regulon and cell-cell communication analysis, and validation of single-cell analysis results.

Single-cell RNA sequencing reveals the suppressive effect of PPP1R15A inhibitor Sephin1 in antitumor immunity.

Protein phosphatase 1 regulatory subunit 15A (PPP1R15A) is an important factor in the integrated stress response (ISR) in mammals and may play a crucial role in tumorigenesis. In our studies, we found an inhibitor of PPP1R15A, Sephin1, plays a protumorigenic role in mouse tumor models. By analyzing the single-cell transcriptome data of the mouse tumor models, we found that in C57BL/6 mice, Sephin1 treatment could lead to higher levels of ISR activity and lower levels of antitumor immune activities.

PI3Kα inhibitor CYH33 triggers antitumor immunity in murine breast cancer by activating CD8T cells and promoting fatty acid metabolism.

Background: The phosphatidylinositol 3-kinase (PI3K) is frequently hyperactivated in cancer and plays important roles in both malignant and immune cells. The effect of PI3Kα inhibitors on the tumor microenvironment (TME) remains largely unknown. Here, we investigated the modulation of the TME by a clinical PI3Kα-specific inhibitor CYH33.

Construction of H K Sn S /TiO nanocomposites with enhanced visible light-driven photocatalytic performance.

In this paper, a new photocatalyst with TiO nanospheres decorated on ultrathin layered thiostannate H K Sn S ( = 0.5-0.6, HKTS) nanosheets was successfully synthesized by a facile solvothermal method combined with the hydrolysis of tetrabutyl titanate and it was denoted as HKTS/TiO.

Drivers of nitrous oxide accumulation in denitrification biofilters with low carbon:nitrogen ratios.

Heterotrophic denitrification is usually inhibited by insufficient carbon sources; however, the underlying mechanisms responsible for nitrous oxide (NO) accumulation within denitrification at low carbon:nitrogen (C/N) ratios have not been quantified from a molecular level. In this study, five denitrification biofilters were developed and exhibited efficiency (total nitrogen: 18.5%-92.

Genetic associations as indices of nitrogen cycling rates in an aerobic denitrification biofilter used for groundwater remediation.

An aerobic denitrification biofilter (ADB) for groundwater remediation was developed with high removal efficiencies (total nitrogen (TN): 82.3-95.8%; NO3(-)-N: 93.

Sludge granulation and performance of a low superficial gas velocity sequencing batch reactor (SBR) in the treatment of prepared sanitary wastewater.

A sequencing batch reactor (SBR) employing a low superficial gas velocity was used to produce aerobic granular sludge for wastewater treatment. At a gas velocity of 0.0056 m s(-1) sludge containing a mixture of light yellow and black granules with similar functional groups was quickly formed.