Effect of Over-Expression of Zinc-Finger Protein (ZFX) on Self-Renewal and Drug-Resistance of Hepatocellular Carcinoma.

BACKGROUND X-chromosome-coupled zinc finger protein (ZFX) in the Zfy protein family is abundantly expressed in both embryonic and hematopoietic stem cells (HSCs). ZFX exist in various tumor cells and is correlated with proliferation and survival of tumor cells. As a malignant tumor with high invasiveness, hepatocellular carcinoma (HCC) may present resistance against chemotherapy and features of stem cells.

Recent advances in the management of hepatocellular carcinoma.

Purpose: The fifth most common cancer of the gastrointestinal system is liver cancer. It is also one of the most common cancers worldwide. The available treatment options include surgery, percutaneous ablation, and liver transplantation.

MicroRNA-1297 regulates hepatocellular carcinoma cell proliferation and apoptosis by targeting EZH2.

In this study, we suggested the level of miR-1297 was downreguled in the human hepatocellular carcinoma compared to the normal cells. We demonstrate ectopic expression of miR-1297 could significantly suppress hepatocellular carcinoma cells proliferation and enhance the cell apoptosis. In vitro reporter assay suggested EZH2 is a direct target gene of miR-1297.

Imbalance of tumor necrosis factor-α, interleukin-8 and interleukin-10 production evokes barrier dysfunction, severe abdominal symptoms and psychological disorders in patients with irritable bowel syndrome-associated diarrhea.

The present study aimed to explore the correlation between cytokine expression of tumor necrosis factor α (TNF‑α), interleukin (IL)‑8 and IL‑10 with occludin production, abdominal symptoms and psychological factors in patients with irritable bowel syndrome‑associated diarrhea (IBS‑D). A total of 42 IBS‑D patients and 20 healthy controls were included, which were recruited from QiLu Hospital in China. ELISA and immunohistochemical analysis were performed for evaluating the cytokines (TNF‑α, IL‑8 and IL‑10) and occludin protein levels in the peripheral blood mononuclear cells (PBMCs) of all subjects.

Efficacy and safety of anticoagulation therapy with different doses of enoxaparin for portal vein thrombosis in cirrhotic patients with hepatitis B.

Background: Patients with cirrhosis have a high incidence of portal vein thrombosis (PVT), and optimal management of PVT in cirrhotic patients remains unclear. Currently, there is no paper on optimal doses of enoxaparin for the management of PVT with cirrhosis.

Aims: To evaluate the efficacy and safety of anticoagulation therapy with different doses of enoxaparin for PVT in cirrhotic patients with hepatitis B.

The analysis of gamma-glutamyl transpeptidase gene in different type liver tissues.

Aim: To probe the value of gamma-glutamyl transpeptidase (GGT) messenger RNA in monitoring canceration of liver cells and for early diagnosis of hepatocellular carcinoma (HCC), by researching the types of GGT messenger RNA (GGTmRNA) in liver tissues and peripheral blood of different hepatopathy.

Methods: The three types of GGTmRNA (A, B, C) in liver tissues and peripheral blood from the patients with HCC, noncancerous hepatopathy, hepatic benign tumor, secondary carcinoma of liver, and healthy persons were detected by reverse-transcription polymerase chain reaction (RT-PCR).

Results: (1) In normal liver tissues, type A was predominantly found (100.

Amplification of screening sensitivity through selective destruction: theory and screening of a library of carbonic anhydrase inhibitors.

A new method for identifying enzyme inhibitors is to conduct their synthesis in the presence of the targeted enzyme. Good inhibitors form in larger amounts than poorer ones because the binding either speeds up synthesis (target-accelerated synthesis) or shifts the synthesis equilibrium (dynamic combinatorial libraries). Several groups have successfully demonstrated this approach with simple systems, but application to larger libraries is challenging because of the need to accurately measure the amount of each inhibitor.

Homogeneous Catalytic Hydrosilylation of Pyridines.

The color change from yellow to violet allows the formation of the intermediate [Cp Ti(SiHMePh)(pyridine)] to be monitored in the reaction of PhMeSiH with a pyridine in the presence of the precatalyst [Cp TiMe ] [Eq. (a)]; the hydride [Cp TiH] is postulated to be the key intermediate in the catalytic cycle. The regioselective hydrosilylation of pyridine and also substituted pyridines can be catalyzed by the titanocene [Cp TiMe ].