Deciphering the role of LncRNA in alcoholic liver disease: Mechanisms and therapeutic potential.

Alcoholic liver disease (ALD) is a spectrum of liver damage caused by chronic alcohol consumption. The disease progresses in stages, starting with simple fatty liver, progressing to alcoholic hepatitis and potentially leading to fibrosis and cirrhosis. The pathophysiology of ALD is complex and involves several cellular and molecular mechanisms.

SUBSTANDARD STARCH GRAIN7 regulates starch grain size and endosperm development in rice.

Starch is synthesized as insoluble, semicrystalline particles within plant chloroplast and amyloplast, which are referred to as starch grains (SGs). The size and morphology of SGs in the cereal endosperm are diverse and species-specific, representing a key determinant of the suitability of starch for industrial applications. However, the molecular mechanisms modulating SG size in cereal endosperm remain elusive.

Rice LIKE EARLY STARVATION1 cooperates with FLOURY ENDOSPERM6 to modulate starch biosynthesis and endosperm development.

In cereal grains, starch is synthesized by the concerted actions of multiple enzymes on the surface of starch granules within the amyloplast. However, little is known about how starch-synthesizing enzymes access starch granules, especially for amylopectin biosynthesis. Here, we show that the rice (Oryza sativa) floury endosperm9 (flo9) mutant is defective in amylopectin biosynthesis, leading to grains exhibiting a floury endosperm with a hollow core.

A Novel Type of Pseudo-Decoupling Method for Two Degree-of-Freedom Piezoelectrically Driven Compliant Mechanisms Based on Elliptical Parameter Compensation.

At present, a large number of two-degree-of-freedom piezoelectrically driven compliant mechanisms (2-DOF PDCMs) have been widely adopted to construct various elliptical vibration machining (EVM) devices employed in precisely fabricating functional micro-structured surfaces on difficult-to-cut materials, which have broad applications in many significant fields like optical engineering and precision manufacturing. For a higher precision of conventional 2-DOF PDCMs on tracking elliptical trajectories, a novel type of pseudo-decoupling method is proposed based on phase difference compensation (PDC). With finite element analysis (FEA), the dependences of elliptical trajectory tracking precision on PDC angles will then be investigated for optimizing PDC angles under different elliptical parameters.

MicroRNA miR-181d-5p regulates the MAPK signaling pathway by targeting mitogen-activated protein kinase 8 (MAPK8) to improve lupus nephritis.

Background: Lupus nephritis (LN) is a common immune disease. The microRNA (miR)-181d-5p is a potential target for treating kidney injury. However, the therapeutic role of miR-181d-5p in LN has not been investigated.

One-Step Fabrication Bioinspired Flexible Hierarchical Micro-Nano Structures with Different Morphologies.

In this study, different flexible structures that are morphologically like the micro-nano pillars, setae, and cilia on many natural organism surfaces are created with a novel fabricating strategy to explore the phenomenon and mechanism of static and dynamic droplets forming on them. Just by adjusting the mold pattern during fabrication, different micro/nanomorphologies including micro-nano pillar, filament, or flake arrays could be conveniently obtained on a pristine smooth film surface. Due to the existence of uniformly distributed hierarchical micro-nano structure arrays that are composed of top-down nanoscale filamentous tips, micro block bases, and grooves on the film, air trapped in arrays connects the atmosphere continuously and forms a successive air-pocket layer, which greatly reduces the solid-liquid interfacial fraction and endows the micro-nanotextured film with the capability of superhydrophobicity, low-adhesion, self-cleaning, anti-icing, and deicing characteristics.

First report of causing anthracnose on peanut ( L.) in China.

Peanut ( L.) is an important cash crop and oil crop around the world. In August 2021, symptoms of leaf spot were found on nearly 50% of peanut plants in the peanut planting base of Xuzhou Academy of Agriculture Sciences, Jiangsu, China.

A Novel Type of Boundary Extraction Method and Its Statistical Improvement for Unorganized Point Clouds Based on Concurrent Delaunay Triangular Meshes.

Currently, three-dimensional (3D) laser-scanned point clouds have been broadly applied in many important fields, such as non-contact measurements and reverse engineering. However, it is a huge challenge to efficiently and precisely extract the boundary features of unorganized point cloud data with strong randomness and distinct uncertainty. Therefore, a novel type of boundary extraction method will be developed based on concurrent Delaunay triangular meshes (CDTMs), which adds the vertex-angles of all CDTMs around a common data point together as an evaluation index to judge whether this targeted point will appear at boundary regions.

DrugDevCovid19: An Atlas of Anti-COVID-19 Compounds Derived by Computer-Aided Drug Design.

Since the outbreak of SARS-CoV-2, numerous compounds against COVID-19 have been derived by computer-aided drug design (CADD) studies. They are valuable resources for the development of COVID-19 therapeutics. In this work, we reviewed these studies and analyzed 779 compounds against 16 target proteins from 181 CADD publications.

Resveratrol ameliorates nutritional steatohepatitis through the mmu‑miR‑599/PXR pathway.

The aim of the present study was to elucidate the effect of resveratrol on non‑alcoholic steatohepatitis (NASH), and the molecular basis in mice and Hepa1‑6 cells, in order to verify its therapeutic effect. C57BL/6J mice were fed a methionine‑choline‑deficient (MCD) diet to induce steatohepatitis and were treated with resveratrol. Mouse sera were collected for biochemical analysis and enzyme‑linked immunosorbent assay, and livers were obtained for histological observation, and mmu‑microRNA (miR)‑599 and inflammation‑related gene expression analysis.

Consensus on the secondary prevention of primary liver cancer.

To standardize the effective prevention, surveillance, and diagnosis of primary liver cancer, the Chinese Society of Hepatology, Chinese Medical Association, invited clinical experts and methodologists to develop the Consensus on the Secondary Prevention of Primary Liver Cancer, which was based on the clinical and scientific advances on hepatocellular carcinoma. The purpose is to provide a current basis for the prevention, surveillance, and early diagnosis of primary liver cancer in patients with chronic liver diseases.

A correlation analysis of the serum hepcidin concentrations and viral loads in HCV-infected patients.

Objective: To investigate the correlation between the serum hepcidin levels and the viral loads in hepatitis C virus (HCV) infected patients.

Methods: Sixty HCV-infected patients (the study group) and 50 healthy controls (the control group) were recruited as the study cohort. The liver function and inflammation-related parameters were compared, and the 60 HCV patients were divided into mild (G1-G2), moderate (G3), and severe (G4) groups according to each patient's inflammatory activity grade (G).

Co-administration of obeticholic acid and simvastatin protects against high-fat diet-induced non-alcoholic steatohepatitis in mice.

Non-alcoholic steatohepatitis (NASH) has no approved therapy. The farnesoid X nuclear receptor (FXR) agonist obeticholic acid (OCA) has shown promise as a drug for NASH, but can adversely affect plasma lipid profiles. Therefore, the present study aimed to investigate the effects and underlying mechanisms of OCA in combination with simvastatin (SIM) in a high-fat diet (HFD)-induced model of NASH.

The Chinese Society of Hepatology position statement on the redefinition of fatty liver disease.

Fatty liver disease associated with metabolic dysfunction is of increasing concern in mainland China, the world's most populous country. The incidence of fatty liver disease is highest in China, surpassing the incidence in European countries and the USA. An international consensus panel recently published an influential report recommending a novel definition of fatty liver disease associated with metabolic dysfunction.

ENLARGED STARCH GRAIN1 affects amyloplast development and starch biosynthesis in rice endosperm.

Cereal crops accumulate large amounts of starch which is synthesized and stored in amyloplasts in the form of starch grains (SGs). Despite significant progress in deciphering starch biosynthesis, our understanding of amyloplast development in rice (Oryza sativa) endosperm remains largely unknown. Here, we report a novel rice floury mutant named enlarged starch grain1 (esg1).

CD14 monocytes and CD163 macrophages correlate with the severity of liver fibrosis in patients with chronic hepatitis C.

Hepatic fibrosis is a crucial pathological process involved in the development of chronic hepatitis C (CHC) and may progress to liver cirrhosis and hepatocellular carcinoma. Activated peripheral blood monocytes and intrahepatic macrophages further promote hepatic fibrogenesis by releasing proinflammatory and profibrogenic cytokines. The present study aimed to investigate the role of peripheral CD14 monocytes and intrahepatic CD163 macrophages in hepatitis C virus (HCV)-associated liver fibrosis and clarify whether serum soluble CD163 (sCD163) may serve as a fibrosis marker in patients with CHC.

Exploration and validation of a novel prognostic signature based on comprehensive bioinformatics analysis in hepatocellular carcinoma.

The present study aimed to construct a novel signature for indicating the prognostic outcomes of hepatocellular carcinoma (HCC). Gene expression profiles were downloaded from Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC) databases. The prognosis-related genes with differential expression were identified with weighted gene co-expression network analysis (WGCNA), univariate analysis, the least absolute shrinkage and selection operator (LASSO).

Glutamate-Cysteine Ligase Catalytic Subunit Attenuated Hepatitis C Virus-Related Liver Fibrosis and Suppressed Endoplasmic Reticulum Stress.

The study aimed to clarify the role and molecular mechanism of glutamate-cysteine ligase catalytic subunit (GCLC) in modulating Hepatitis C virus (HCV)-related liver fibrosis. Twenty patients with HCV-related liver fibrosis and 15 healthy controls were enrolled. Differentially expressed plasma mRNAs were detected by digital gene expression profile analysis and validated by qRT-PCR.

Clusterin contributes to hepatitis C virus-related hepatocellular carcinoma by regulating autophagy.

Aims: To explore the potential regulatory mechanism of differentially expressed mRNAs in Hepatitis C virus (HCV)-related hepatocellular carcinoma (HCC).

Main Methods: Patients with HCV-related HCC and age- and gender-matched healthy subjects were enrolled. Differentially expressed mRNAs in the plasma were detected by digital gene expression (DGE) profile analysis.

Heme Oxygenase-1 Suppresses Wnt Signaling Pathway in Nonalcoholic Steatohepatitis-Related Liver Fibrosis.

Methods: Mice were fed with a methionine-choline-deficient (MCD) diet for 8 weeks to induce steatohepatitis-related liver fibrosis and were treated with HO-1 inducer Hemin and inhibitor ZnPP. Mouse sera were collected for the biochemical analysis, and livers were obtained for further histological observation and gene expression analysis. HSC-T6 cells were cultured for the study and were administrated with Hemin and si-HO-1 to induce or inhibit the expression of HO-1.

The effect of size and surface ligands of iron oxide nanoparticles on blood compatibility.

Superparamagnetic iron oxide nanoparticles (SPIONs) have been widely used and have attracted increased attention for their unique physicochemical properties, especially in biomedical sciences as contrast agents following intravenous administration. However, only few studies have systematically reported the blood compatibility of iron oxide nanoparticles with different physicochemical properties such as different sizes and surface ligands. Therefore, we selected three widely used organic ligands (polyacrylic acid, hyaluronic acid, and chitosan) with modified SPIONs at the same size of 5-6 nm, and polyacrylic acid-modified SPIONs with different sizes (5, 10, and 30 nm) at different concentrations to evaluate their haemocompatibility.

Emperipolesis mediated by CD8 T cells correlates with biliary epithelia cell injury in primary biliary cholangitis.

Primary biliary cholangitis (PBC) is an autoimmune disease characterized by chronic destruction of the bile ducts. A major unanswered question regarding the pathogenesis of PBC is the precise mechanisms of small bile duct injury. Emperipolesis is one of cell-in-cell structures that is a potential histological hallmark associated with chronic hepatitis B.

A New Approach to Decoupled Non-Resonant Polishing.

Two-dimensional vibration-assisted polishing (2D-VAP) is a new polishing approach proposed in recent years, which is considered to be very suitable for the polishing of micro-optical parts and micro-structured surfaces. The advantages of the 2D-VAP approach are as follows: A very high relative velocity can be obtained between the workpiece and the polishing tool; the desired motion trajectory can be formed in one polishing cycle. However, there are still some problems to be solved in the 2D-VAP approach, mainly involving: The vibration frequency of the resonant excitation cannot be regulated, which makes it difficult to adapt to the processing demands of different materials; the theoretical model of removal function has been studied in few papers; and motion coupling occurs easily between the horizontal and vertical directions, which affects the trajectory synthesized at the polishing tool.