Publications by authors named "Rong-qing Pang"
Background: The tumorigenesis of infused umbilical cord mesenchymal stem cells (UC-MSCs) is being preclinically evaluated.
Methods: We observed tumor formation in NOD SCID mice after a single subcutaneous injection of hUC-MSCs and the effect of these cells on tumor growth in tumor-bearing mice. Three generations (P5, P7, and P10) of hUC-MSCs (1 × 10) from two donors (hUC-MSC1 and hUC-MSC2) were inoculated subcutaneously into NOD SCID mice.
Article Synopsis
- The study investigates the effects of mouse umbilical cord mesenchymal stem cells (mUCMSCs) on the reproductive health of ageing C57 mice, focusing on their potential to repair ovarian function.
- Aging mice with diminished ovarian function received mUCMSC injections, resulting in increased ovarian size, the presence of follicles, and enhanced hormone levels compared to a control group.
- The findings indicate that mUCMSCs can reduce cell apoptosis and oxidative stress in granulosa cells by altering specific gene expressions and activating key cellular pathways, suggesting a potential for regenerative therapies in reproductive health.
Based on the characteristics of modern weapon injury, a repetitive model of traumatic systemic inflammatory response syndrome (SIRS) and an evaluation system were established. The models were treated with GFP-labeled tree shrew umbilical cord mesenchymal stem cells (UCMSCs). Forty out of 50 tree shrews were used to make a unilateral femoral comminuted fracture.
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- Ischemia-reperfusion injury significantly contributes to acute kidney injury and affects recovery after kidney transplants; this study explores the potential protective role of stem cell transplantation in such injuries.
- The experiment involved 40 rabbits divided into four groups, with 30 establishing the injury model, and 10 serving as untreated controls; comparisons were made based on treatments with either induced or non-induced peripheral blood mononuclear cells (PBMCs).
- Results showed that rabbits treated with induced PBMCs exhibited improved renal function and less severe pathological changes, indicating the therapeutic potential of these induced stem cells in mitigating kidney injury.
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- A mouse model for allergic rhinitis (AR) was developed using BALB/c mice to aid in future research on the condition.
- * The study involved preparing human umbilical cord mesenchymal stem cells (hUCMSCs), which were shown to be effective for tracking in animal studies and possibly for AR treatment.
- * The transplantation of hUCMSCs, particularly through tail vein injection, demonstrated positive effects on the AR model, with a significant presence of these cells in the nasal area after two weeks.
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- The study aimed to investigate how allogeneic umbilical cord mesenchymal stem cell (mUCMSC) transplantation affects the thymus in aging mice, potentially offering a new treatment for age-related thymic atrophy.
- Results indicated that mUCMSC treatment improved thymus structure and function by analyzing changes in thymic tissue and immune cell markers, as well as promoting hair regeneration in the mice.
- The findings suggest that mUCMSCs can reduce thymus aging by affecting specific senescence-related genes, though further research is needed to fully understand the underlying mechanisms.
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- Inflammatory bowel disease (IBD) causes chronic inflammation in the gastrointestinal tract, and researchers are exploring new treatments with minimal side effects.
- Mouse models of IBD were created using a solution called DSS, allowing scientists to test the effects of umbilical cord mesenchymal stem cells (UCMSCs) from both mice and humans.
- The study found that UCMSCs improve survival rates and promote healing by enhancing key proteins in the intestines, offering a promising avenue for future IBD therapies.
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- - The study explored the link between bone marrow mesenchymal stem cells (BMSCs) and aging by establishing an aging macaque model, analyzing how BMSCs from young and aged macaques differ in their characteristics.
- - Researchers found that aging BMSCs showed decreased levels of key genes associated with aging, such as TERT, SIRT1, and SIRT6, using techniques like RT-PCR and Western blot.
- - The team compared the effects of young and aged BMSCs on 293T cells, investigated secreted cytokines, and analyzed transcriptomes of peripheral blood mononuclear cells from macaques to understand gene transcription and regulatory mechanisms related to aging.
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- Umbilical cord mesenchymal stem cells (UC-MSCs) demonstrate strong potential to improve survival and mitigate damage from acute radiation injury in tree shrews.
- The treatment resulted in significant reductions in lung inflammation, apoptosis, and fibrosis, alongside enhanced blood counts and hematopoiesis.
- Analysis indicated increased levels of anti-inflammatory cytokines and growth factors in treated animals, while inflammatory and fibrosis-related factors decreased, suggesting UC-MSCs could be a promising therapy for radiation injuries.
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- This study evaluates the safety and immunological effects of umbilical cord mesenchymal stem cells from cynomolgus monkeys (mUC-MSCs) to guide human clinical applications.
- Eighteen cynomolgus monkeys were split into three groups to assess the impact of different dosages, with observations made for any toxic reactions after repeated cell administrations.
- Results showed no deaths or serious health issues, with some minor changes in certain blood levels that normalized after 28 days, suggesting a safe dosage for potential human use is significantly higher than current clinical amounts.
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- Researchers are exploring a novel cell transplantation method using reprogrammed peripheral blood mononuclear cells (PBMCs) turned into induced mesenchymal stem cells (iMSCs) for treating chronic renal insufficiency.
- An animal model of chronic renal insufficiency, created through unilateral ureteral obstruction, was used to evaluate the effectiveness of these iMSCs compared to non-induced PBMCs.
- The study aims to assess the therapeutic potential of iMSC transplantation in managing chronic renal issues, hoping to provide new treatment options.
Article Synopsis
- * This study explored the use of islet-like cells from umbilical cord mesenchymal stem cells (UC-MSCs) in tree shrews with type 2 diabetes, showing effectiveness in reducing disease severity.
- * UC-MSCs were found to differentiate into functional insulin-secreting cells through stimulation from high glucose and activation of Notch signaling, making this method a potentially simple and cost-effective diabetes treatment.
Background: The establishment of a tree shrew model for systemic lupus erythematosus (SLE) provides a new method to evaluate the pathogenesis of autoimmune diseases.
Methods: Eighty tree shrews were randomly divided into four groups receiving either an intraperitoneal injection of pristane, lipopolysaccharide (LPS), or pristane and LPS, or no injection. Three weeks after injection, the SLE model tree shrews were divided into the model group and the treatment group.
The aim of this study was to establish a tree shrew metabolic syndrome model and demonstrate the utility of MSCs in treating metabolic syndrome. We used tree shrew umbilical cord mesenchymal stem cell (TS-UC-MSC) transplantation for the treatment of metabolic syndrome to demonstrate the clinical application of these stem cells and to provide a theoretical basis and reference methods for this treatment. Tree shrew metabolic syndrome model showed significant insulin resistance, high blood sugar, lipid metabolism disorders, and hypertension, consistent with the diagnostic criteria.
View Article and Find Full Text PDFWe previously found that chicken egg white extract could promote cell survival and proliferation. In the present study, we further separated this extract into its components to identify those primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken egg white extract by ultrafiltration and 293T cell cultures were supplemented with various concentrations.
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- Embryonic-like stem cells (ELSCs) show potential in muscle regeneration, expressing markers for embryonic stem cells and differentiating into various cell types more effectively than mesenchymal stromal cells (MSCs).
- In a study using dystrophin/utrophin double knock-out mice (a model for Duchenne muscular dystrophy), ELSCs were injected via tail vein and led to improved motor function and reduced creatine kinase levels two months post-transplant.
- Results indicated that ELSCs enhanced dystrophin expression and muscle tissue quality compared to MSCs, supporting their potential for therapeutic use in muscle disorders.
Diabetic nephropathy (DN) is a common microvascular complication of diabetes. We used a new DN model in tree shrews to validate the use of bone-marrow mesenchymal stem cell (BM-MSC) transplantation to treat DN. The DN tree shrew model was established by a high-sugar and high-fat diet and four injections of streptozotocin.
View Article and Find Full Text PDFBone marrow mesenchymal stem cells (BMSCs) are self-renewing, multipotent cells that can migrate to pathological sites and thereby provide a new treatment in diabetic animals. Superparamagnetic iron oxide/4',6-diamidino-2-phenylindole (DAPI) double-labeled BMSCs were transplanted into the pancreatic artery of macaques to treat type 2 diabetes mellitus (T2DM). The treatment efficiency of BMSCs was also evaluated.
View Article and Find Full Text PDFBackground: Systemic lupus erythematosus (SLE) is a multisystem disease that is characterized by the appearance of serum autoantibodies. No effective treatment for SLE currently exists.
Methods: We used human umbilical cord mesenchymal stem cell (H-UC-MSC) transplantation to treat B6.
Introduction: Renal interstitial fibrosis (RIF) is a significant cause of end-stage renal failure. The goal of this study was to characterize the distribution of transplanted induced autologous stem cells in a rabbit model of renal interstitial fibrosis and evaluate its therapeutic efficacy for treatment of renal interstitial fibrosis.
Methods: A rabbit model of renal interstitial fibrosis was established.
Article Synopsis
- Induced multipotent stem (iMS) cells can be created from somatic cells through genetic or epigenetic modifications, and fish oocyte extracts (FOE) have previously been shown to successfully induce skin fibroblast cells into iMS cells.
- This study explores whether FOE can also induce mouse peripheral blood mononuclear cells (PBMCs) into the iMS state and assesses their survival after transplantation into irradiated female mice.
- Results indicated that FOE-induced PBMCs not only exhibited stem cell markers but also led to significantly higher spleen wet weights and colony counts in the recipient mice, with the presence of Y chromosomes confirming the survival of the transplanted cells.
Article Synopsis
- The study explored the impact of induced autologous stem cells on blood sugar levels in rabbits with type 1 diabetes by transplanting dedifferentiated fibroblasts into the pancreatic artery.
- After four weeks, the treatment group showed significantly lower blood glucose levels and increased plasma insulin and C-peptide levels, indicating improved pancreatic function.
- The morphological analysis revealed that induced stem cells led to changes in islet cell structure, with better preservation and function compared to the non-induced treatment group and control group, suggesting potential for diabetes therapy.
The objective of this study was to investigate the effects of transplanted bone marrow mesenchymal stem cells (BMSCs) administered via internal jugular vein injection, carotid artery injection, or intraventricular transplantation for the treatment of cerebral infarction, which was modeled in rats. The neurological scores of the treated rats and the distribution of the transplanted cells (GFP-labeled) in the infarction area were evaluated. The cerebral infarction model was produced by inserting a modified Zea-longa suture, which generated middle cerebral artery occlusion (MCAO).
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- The study investigated how different egg extracts affect the survival and differentiation of spleen cells from male GFP-transgenic mice after being permeabilized for 30 minutes.
- Among the five egg extracts tested, chicken-egg-white and whole-egg extracts significantly enhanced the survival and differentiation of the spleen cells compared to others, with GFP-positive cells detected in female mice post-transplantation.
- The findings suggest that chicken-egg-white and whole-egg extracts could be valuable in future cellular biology research, particularly in maintaining stem cell function.