Selecting and Characterizing Tyrosinase Inhibitors from Rhizoma Based on Spectrum-Activity Relationship and Molecular Docking.

Rhizoma (AMR) is a famous classical Chinese traditional medicine (CTM), which has been used as a tonic for many diseases for thousands of years. In ancient China, it was used as a supplementary food for beauty in the palace. In preliminary studies, the function of whitening skin and the significant inhibiting effect on tyrosinase (TYR) which is the reactive enzyme in the composition of melanin of AMR were discovered, and the relevant research was rarely reported.

[Determination and analyse of soil fertility of Pseudostellariae Radix planting base of Shibing County and Huangping County in Guizhou Province].

Objective: To determine soil fertility of the Pseudostellariac Radix planting base of Shibing County and Huangping County in Guizhou Province, and to provide experimental basis for soil improvement and balanced fertilization.

Methods: 12 soil samples from Shibing County and 10 soil samples from Huangping County were involved in the detection.

Results: In the soil samples from Shibing County, the contents of organic matter, total N, available N, total P, available P and available K were higher, while the content of total P was at middle level.

[Study on HPLC fingerprint of miao medicine Ardisia japonica].

Objective: To establish the HPLC fingerprint of Ardisia japonica.

Methods: HPLC analysis was performed on a Diamonsil C18 column (250 mm x 4.6 mm, 5 microm) with the eluting system of gradient consisted of methanol-0.

[Study on immunological effect of housefly protein in mice].

Objective: To study the immunological effect of the housefly protein in mice.

Methods: The housefly protein was given orally in normal and Cyclophosphamide (CY) immunosuppressive mice, and then the indexes of the clearance rate of carbon particles and the serum hemolysin against SRBC were detected.

Results: The housefly protein could increase HC50 significantly in the normal mice, but could not in the CY immunosppressive mice.

[Isolation and purification of antibacterial peptides from the larvae secretion of housefly and the characteristics].

Objective: To isolate and purify the antibacterial peptides from larvae secretion of housefly (Musca domestica) and study their partial characteristics.

Methods: Protein isolation and purification were performed by routine process, namely, ultrafiltration, solid phase extraction (SPE) and reversed-phase high-performance liquid chromatography (RP-HPLC). The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the antibacterial peptides were examined.