Highly sensitive detection of cancer-related genes based on complete fluorescence restoration of a molecular beacon with a functional overhang.

The accurate detection of cancer-related genes is of great significance for early diagnosis and targeted therapy of cancer. In this contribution, an automatically cycling operation of a functional overhang-containing molecular beacon (OMB)-based sensing system was proposed to perform amplification detection of the p53 gene. Contrary to the common molecular beacon (MB), a target DNA is designated to hybridize with a label-free recognition probe (RP) with a hairpin structure rather than OMB.

[Immune regulation of T-bet adjuvant on Ag85B DNA vaccine against Mycobacterium tuberculosis].

Aim: To construct a novel Ag85B DNA vaccine based on the genetic adjuvant of T-bet known as Th1 transcription factor and to research the immunoregulation function of the DNA complex vaccine.

Methods: Ag85B gene and T-bet gene were amplified by RT-PCR, and cloned into pcDNA3.1 plasmid to construct recombinant plasmids pcDNA3-Ag85B and pcDNA3-T-bet, respectively.

[Effect of epitope-based peptide-DNA dual vaccines against Schistosoma japonicum in mice].

A C-T-B PDDV mixture of the three constructed epitope-based peptide-DNA dual vaccines (PDDV) containing the CTL (C), Th (T) and B-cell (B) epitopes from Sj22.6 tegument (C-PDDV, T-PDDV and B-PDDV) with a 1:1:1 ratio was prepared. Thirty-six mice were randomly divided into six groups averagely named as 18K group, PBS group, C-PDDV group, T-PDDV group, B-PDDV group, and C-T-B PDDV group.

Th1 immunity is not required for the effect of lipopolysaccharide exposure on modifying asthmatic responses of mice before sensitization.

Background: Disequilibrium of Th1/Th2 is known as an important cause of allergic asthma with a biased Th2 type response. It has been shown that lipopolysaccharide (LPS) administration during post-sensitization modified the inflammation of asthma via upregulating the Th1 response that decrease the Th2 immunity. We would like to know if, during pre-sensitization, the elevated Th1 response is necessary for LPS exposure to modify the asthmatic response.

[The effect of rural exposure on allergic asthma in Anhui province].

Objective: To establish the evidence of exposure to rural areas would reduce the risk of atopic asthma and sensitization.

Methods: A cross-sectional survey was carried out in 2986 school-age children and their parents completed standardized questionnaires on atopic asthma and sensitization, wheezing. A radioallergosorbent technique-fluorescence enzyme immunoassay (RAST-FEIA) was used to measure the level of specific IgE in serum.

[Study on the relations between concentration of endotoxin in dwelling and atopic asthma in school-age children].

Objective: To explore the effect of endotoxin concentration in dwellings on the prevalence of atopic asthma in children.

Methods: Standardized questionnaires of asthma were distributed to the parents of 2986 school children aged between 8 and 12 years and endotoxin content in children's mattress was measured by a kinetic limulus assay. A radioallergosorbent technique--fluorescence enzyme immunoassay (RAST-FEIA) was used to measure the level of specific IgE in serum.

Diagnosis of intestinal acariasis with avidin-biotin system enzyme-linked immunosorbent assay.

Aim: To explore the value of avidin-biotin system enzyme-linked immunosorbent assay (ABC-ELISA) in diagnosis of intestinal acariasis.

Methods: Mite-specific IgG levels in serum of 48 patients with intestinal acariasis were measured with ABC-ELISA. The sensitivity of this method was compared with that of staphylococcal protein A enzyme-linked immunosorbent assay (SPA-ELISA).

Clinical and epidemiological features of patients with clonorchiasis.

Aim: To study the clinical and epidemiological features of patients with clonorchiasis so as to provide scientific evidences for the diagnosis and prevention of clonorchiasis.

Methods: Stools from 282 subjects suspected of having clonorchiasis were examined for helminth eggs with modified Kato's thick smear and sedimentation methods, and their sera were tested for HAV-DNA, HBV-DNA, HCV-RNA, HDV-RNA and HEV-RNA with polymerase chain reaction (PCR). Clinical symptoms of patients with clonorchiasis only were analyzed, and their blood samples were tested for circulating antigen (CAg) with Dot-ELISA, eosinophilic granulocyte count, and alanine aminotransferase (ALT).