Publications by authors named "Rong-Zhi Yang"
Development of a 9-locus X-STR multiplex PCR system for genetic analysis of three ethnic populations in China.
J Huazhong Univ Sci Technolog Med Sci
April 2015
X-chromosome short tandem repeats (X-STR) analysis has been confirmed to be effective for kinship testing such as in deficiency paternity cases. The aim of this study was to develop a new multiplex polymerase chain reaction (PCR) system that can simultaneously amplify 9 X-STR loci (GATA172D05, DXS10159, DXS6797, HPRTB, DXS10079, DXS6789, DXS9895, DXS10146 and GATA31E08) in the same PCR reaction, and to obtain the database of the 9 X-STR loci in three ethnic populations in China. The genetic data of 815 (404 females and 411 males) unrelated Han Chinese from Hubei province, and Yi and Zhuang Chinese from Yunnan province were analyzed by using this multiplex system.
View Article and Find Full Text PDFThere is no available method of age-prediction for biological samples. The accumulating evidences indicate that DNA methylation patterns change with age. Aging resembles a developmentally regulated process that is tightly controlled by specific epigenetic modifications and age-associated methylation changes exist in human genome.
View Article and Find Full Text PDFAge-prediction is an important part of forensic science. There is no available method of individual age-prediction for general forensic biological samples at crime scenes. Accumulating evidence indicates that aging resembles a developmentally regulated process tightly controlled by specific age-associated methylation exists in human genome.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to identify polymorphic Y chromosome markers in the Chinese Han population and gather related genetic data.
- Genotyping was performed on 23 Y chromosome markers in 160 unrelated Chinese males using specialized PCR and electrophoresis techniques, revealing genetic polymorphism in 20 markers.
- A total of 35 haplogroups were identified, indicating high genetic diversity, which can be useful for forensic applications and understanding population evolution.
Objective: The genetic polymorphism of two STR loci, D20S85 and D6S477, were studied in 280 unrelated Chinese individuals in Wuhan.
Methods: The PCR amplified products were analyzed by PAGE and silver staining.
Results: 10 and 9 alleles were observed in these two STR loci, and the discriminating power (DP) were 0.