[Expression of DUOX2 in psoriasis and atopic dermatitis lesion].

Objective: To investigate the expression of dual oxidase 2 (DUOX2) in psoriasis vulgaris lesions, atopic dermatitis (AD) lesions and normal skin and its role in cutaneous anti-inflammation.

Methods: Tissue samples were harvested from psoriasis lesion area, psoriasis non-lesion area, AD lesion area and AD non-lesion area, as well as normal skin, the expression level of DUOX2 protein was detected by immunohistochemical staining. The mRNA level of DUOX2 was detected by reverse transcription polymerase chain reaction (RT-PCR) analysis.

[Effects of TNF-alpha, IFN-gamma on the ability of keratinocytes to kill intracellular Staphylococcus aureus].

Objective: To study the bactericidal activity of keratinocytes to Staphylococcus aureus in the presence of TNF-alpha, IFN-gamma and their combination.

Methods: The keratinocytes were co-cultured with Staphylococcus aureus to establish the Staphylococcus invasion model of skill cell, then the differenct concentrations of TNF-alpha, IFN-gamma and their combinationt were added into the culture. 24 h later the number of intracellular viable bacteria was counted, while the same amount of sterilized PBS was used as control.