Screening and identifying a novel M-MDSCs-related gene signature for predicting prognostic risk and immunotherapeutic responses in patients with lung adenocarcinoma.

Lung adenocarcinoma (LUAD) shows intratumoral heterogeneity, a highly complex phenomenon that known to be a challenge during cancer therapy. Considering the key role of monocytic myeloid-derived suppressor cells (M-MDSCs) in the tumor microenvironment (TME), we aimed to build a prognostic risk model using M-MDSCs-related genes. M-MDSCs-related genes were extracted from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases.

[Construction and anti-tumor efficacy of a pentameric peptide vaccine that targets S100A8].

Myeloid-derived suppressor cells(MDSC) play critical roles in immune escape of tumor. We hypothesized that elimination of tumor-induced MDSCs might help to block tumor growth. Therefore, we constructed a cholera toxin B based peptide vaccine that targets a MDSC surface marker S100A8.

[Proteomics analysis of serum biomarkers in patients with pathological myopia].

Objective: To identify the correlation between serum-based differentially expressed proteins and pathological myopia.

Methods: It was a case-control study. The serum of 30 pathological myopia patients and 30 age- and gender-matched normal controls were collected from Shanghai First People's Hospital affiliated to Shanghai Jiaotong University.

Comparative analysis of depurination catalyzed by ricin A-chain on synthetic 32mer and 25mer oligoribonucleotides mimicking the sarcin/ricin domain of the rat 28S rRNA and E. coli 23S rRNA.

Ricin A-chain can inactivate eukaryotic ribosomes, but exhibits no N-glycosidase activity on intact E. coli ribosomes. In the present research, in order to avoid using radiolabeled oligoribonucleotides, two kinds of synthetic 5'-FAM fluorescence-labeled oligoribonucleotide substrates were used to mimic the sarcin/ricin domains of rat 28S rRNA and E.

[Raman spectroscopic study of human serum albumin interacting with 3-picolylamine].

Raman spectra of human serum albumin (HSA) and HSA-3-picolylamine complex were obtained. The spectra indicate the configuration and structural transformations of HSA. The results show that the secondary structure is main alpha-helix, and the binding of 3-picolylamine doesn't change the secondary structure.

[A novel microbeads-based chemiluminescence immunoassay for detecting human thyrotropin].

Aim: To establish a low cost and sensitive microbeads-based chemiluminescence immunoassay (CLEIA) for detecting human thyrotropin.

Methods: Thyrotropin in sera was captured by an alkaline phosphase-labeled mAb against TSH beta subunit and a FITC-labeled mAb against TSH alpha subunit. Captured thyrotropin was then isolated with immuno-magnetic beads conjugated with anti-FITC antibody and then quantified by chemiluminescence using adamanatane amine as luminescent substrate.

Cloning and Distribution of rRP58, A Novel Neuronal Gene.

EST (AW055733) is a 3'-cDNA fragment specially expressed in rat brain. It is homologous to human and mouse RP58 gene, which is a transcriptional repressor gene. Primers were designed based on these two genes, and then two transcripts of rRP58 gene were got from male SD rat using RT-PCR method.