YY1-mediated PTEN dephosphorylation antagonizes IR-induced DNA repair contributing to tongue squamous cell carcinoma radiosensitization.

Ionizing radiation (IR) confers a survival advantage in tongue squamous cell carcinoma (TSCC), however, IR resistance limits its efficacy. Although Yin Yang 1 (YY1) has been reported to play a role in genotoxic drug resistance by accelerating DNA repair, its role in TSCC radioresistance remains unclear. In this study, we examined YY1 mRNA and protein expression in human tongue cancer samples using qRT-PCR and western blotting, respectively.

Epigallocatechin-3-gallate inhibits proliferation and induces apoptosis in odontogenic keratocyst keratinocytes.

Objective: The aim of this study was to investigate the effects of epigallocatechin-3-gallate on the proliferation and apoptosis of odontogenic keratocyst (OKC) keratinocytes in vitro.

Materials And Methods: Keratinocytes isolated from the epithelial lining of the OKC were cultured in keratinocyte serum-free medium and identified by CK10, CK14, pan-cytokeratin and vimentin immunofluorescence staining. The cells were exposed to EGCG at different concentrations, and proliferation inhibition was measured by cell counting kit 8 assay.

[Expression and clinical significance of the genes of Wnt signaling pathway in keratocystic odontogenic tumor of the jaw bones].

Purpose: To study the role of genes of Wnt signaling pathway in keratocystic odontogenic tumor (KCOT) of the jaw bones.

Methods: Fresh specimens of KCOT and the same patient 's normal oral mucosa were obtained. Then RNA was extracted.

[The effect evaluation of suction drainage to prevent fistula after superficial parotidectomy].

Purpose: To evaluate the clinical value of suction drainage to prevent fistula after parotidectomy, and seek the best opportunity to remove the drainage according to the draining output and duration.

Methods: One hundred and ninety-four patients with parotid diseases after superficial parotidectomy were assigned into pressure dressing group and suction drainage group. Pressure dressing was used after suction drainage tube was removed in the pressure group, while suction drainage tube was fixed through the process in the suction group.

Gene mutations in the D-loop region of mitochondrial DNA in oral squamous cell carcinoma.

The present study aimed to investigate gene mutations in the displacement‑loop (D‑loop) region of mitochondrial DNA (mtDNA) in patients with oral squamous cell carcinoma (OSCC) in order to examine the role of gene mutation in mtDNA in OSCC tumorigenesis. mtDNA was obtained from cancer tissues, paracancerous tissues and normal mucosal tissues of thirty patients with OSCC. The D‑loop region of the mtDNA was amplified using polymerase chain reaction, sequenced and then analyzed by Chromas software and BLAST to identify the mutation sites.

[Screening of the gene mutation in D-loop region of mitochondrial DNA in oral squamous cell carcinoma].

Objective: To investigate the gene mutation in D-loop region of mitochondrial DNA (mtDNA) in oral squamous cell carcinoma (OSCC) tissue and to explore the role of the gene mutation in D-loop region in the OSCC tumorigenesis.

Methods: mtDNA was obtained from cancer, paracancerous and normal mucosa tissues of thirty patients with OSCC. The D-loop regions of mtDNA were amplified with PCR, sequencing and then analyzed by Chromas software and BLAST to identify the mutation site.

[The mutations of germline succinate dehydrogrnase subunit B (SDHB) in sporadic paragangliomas].

Purpose: The purpose of this study was to investigate the somatic mutations of human mitochondria succinate dehydrogenase subunit B (SDHB) in sporadic paragangliomas.

Methods: Eight exons of SDHB gene in 8 sporadic paragangliomas cases were amplified by PCR and sequenced, respectively. The sequences were analyzed to find mutations compared with human homology sequence in Genebank and SNP database.

[An experimental study of endothelial progenitor cells on bone formation in tissue-engineered bone in dogs].

Purpose: To observe the effects of endothelial progenitor cells (EPCs) on bone formation of tissue engineered bone in dogs.

Methods: Bone marrow stromal cells (BMSCs) and EPCs were derived from dog bone marrow and cultured in different medium in vitro. They were seeded on demineralized bone matrix (DBM) to build tissue engineered bone, then the construct was implanted into the fasciae of latissimus dorsi muscle, the degree of bone formation was analyzed with imaging and histological methods at different time points.

[The mutations of the D-loop hypervariable region II and hypervariable region III of mitochondrial DNA in oral squamous cell carcinoma].

Objective: To investigate the frequency of mitochondrial DNA (mtDNA) D-loop hypervariable region II (HVR II) and hypervariable region III (HVR III) mutations in oral squamous cell carcinoma (OSCC) and their correlation to provide the new targets for the prevention and treatment of OSCC.

Methods: The D-loop HVR II and HVR III regions of mtDNA in seven cases with OSCC tissues, matched with paracancerous tissues and normal mucosa tissues from the same case, were amplified by polymerase chain raction (PCR), then were detected by direct sequencing to find the mutantsites after the comparison of all sequencing results with the mtDNA Cambridge sequence in the GenBank database.

Results: 82 (56 species) nucleotide changes, with 51(26 species) nucleotide polymorphism, were found after the comparison of all sequencing results with the mtDNA Cambridge sequence in the GenBank database.

Effect of platelet-rich plasma and latissimus dorsi muscle flap on osteogenesis and vascularization of tissue-engineered bone in dogs.

Purpose: The present study evaluated the effects of platelet-rich plasma (PRP) and the latissimus dorsi muscle flap on osteogenesis and vascularization of tissue-engineered bone.

Materials And Methods: Bone marrow stromal cells (BMSCs) were subcultured, and PRP was obtained from the same dogs. Demineralized bone matrix (DBM) was prepared from homologous bone.

[The value of single photon emission computed tomography-computed tomography in diagnosis of carcinoma invasion of mandible].

Objective: To investigate the value of single photon emission computed tomography(SPECT)-computed tomography(CT) in diagnosis of oral carcinoma invasion to mandible.

Methods: Thirty-four patients with oral carcinoma invasion to mandible were divided into two groups, group A (surrounding invasion) and group B (central invasion). The edge of the invasion was evaluated by SPECT-CT, CT and pathological examination.

[A comparison between CT and SPECT in detecting mandibular invasion by lower gingival squamous cell carcinoma].

Purpose: The purpose of this study is to compare the value of CT and SPECT in diagnosis of lower gingival carcinoma invading the mandible.

Methods: From February 2002 to October 2006, twenty-one patients with lower gingival squamous cell carcinoma were enrolled.The data of CT and SPECT were studied,and compared with histopathological findings.

[Clinical biological behavior and treatment strategy of hemangioma in oral and maxillofacial region].

Hemangioma can not be prevented until nowadays. Early identification and definite diagnosis are necessary to achieve the best outcome. The clinical biological behaviors of hemangiomas in oral and maxillofacial region are completely investigated and deeply analyzed in this paper.

[Early laser intervention of hemangioma in facial and neck regions of infant].

Objective: The study was to evaluate the method of early laser intervention of hemangioma in facial and neck regions of infant.

Methods: Between January 1999 and December 2006, twelve patients, aged 6 days to 3 months, with cutaneous hemangioma in facial and neck regions, were treated with laser, eight cases with Nd:YAG laser therapy and four cases with Venus laser therapy. Four cases with hemangioma in facial and neck regions of infant treated with oral corticosteroid were as control.

[Osteoma in the soft palate: report of one case and review of the literature].

Osteoma is a benign tumor, which is composed of mature differentiated bone tissue and derived from osteoplast in periosteum endothecium. Osteomas is common in the bone in clinic, however, osteoma in the soft tissue is distinctly rare. Clinically, the lesion presents as hard, slowly growing mass with clear border.

[Effect of platelet rich plasma on vascularization of tissue-engineered bone].

Objective: To study the effects of platelet rich plasma (PRP) on vascularization of tissue-engineered bone.

Methods: Bone marrow stromal cell (BMSC) were isolated from iliac bone of dogs. PRP was obtained from the same dog and demineralized bone matrix (DBM) was prepared from homologous bone.

[Effect of platelet-rich plasma and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs].

Objective: To study the effect of platelet-rich plasma (PRP) and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs.

Methods: Bone marrow stromal cells (BMSCs) were isolated from iliac bone of dogs. PRP was obtained from the same dog.

[Study of MSCs in vitro cultured on demineralized bone matrix of mongrel].

Purpose: To manufacture demineralized bone matrix(DBM) of mongrel and to explore the feasibility of DBM as scaffold of bone tissue engineering.

Methods: Thigh bones of mongrel were degreased, demineralized, deproteined, freezed, dried and sterilized to form DBM. Mesenchymal stem cells(MSCs) were seeded onto the scaffold and their growth were examined by inverted phase contrast microscope and scanning electron microscope.

[Carotid body tumor: a retrospective study of 26 consecutive cases].

Objective: To study the clinical features, diagnoses and operative intervention of carotid body tumors.

Methods: The medical records of 26 patients with carotid body paragangliomas between 1993 and 2004 were retrospectively reviewed.

Results: Two of twenty-six patients had bilateral tumors.