Publications by authors named "Rong-Qi Sun"
Integrated Molecular Characterization of Sarcomatoid Hepatocellular Carcinoma.
Clin Mol Hepatol
December 2024
Background: Sarcomatoid hepatocellular carcinoma (HCC) is a rare histological subtype of HCC characterized by extremely poor prognosis; however, its molecular characterization has not been elucidated.
Methods: In this study, we conducted an integrated multiomics study of whole-exome sequencing, RNA-seq, spatial transcriptome, and immunohistochemical analyses of 28 paired sarcomatoid tumor components and conventional HCC components from 10 patients with sarcomatoid HCC, in order to identify frequently altered genes, infer the tumor subclonal architectures, track the genomic evolution, and delineate the transcriptional characteristics of sarcomatoid HCCs.
Results: Our results showed that the sarcomatoid HCCs had poor prognosis.
Article Synopsis
- - Intrahepatic cholangiocarcinoma (ICC) is the second most common liver cancer, with a poor prognosis and few treatment options, especially among Chinese patients whose genomic features are not well understood.
- - This study analyzes the whole-exome sequencing of 204 ICC samples from Chinese patients, identifying six mutational signatures, including those linked to known harmful substances, and 13 significantly mutated genes such as SAV1.
- - Researchers found that mutations in the SAV1 gene are linked to lower protein levels, increased tumor recurrence, and shorter patient survival, suggesting it plays a tumor-suppressor role that impacts cancer progression through Hippo signaling pathways.
Backgrounds/aims: Intrahepatic cholangiocarcinoma (ICC) is a highly desmoplastic tumor with poor prognosis even after curative resection. We investigated the associations between the composition of the ICC stroma and immune cell infiltration and aimed to develop a stromal-immune signature to predict prognosis in surgically treated ICC.
Methods: We recruited 359 ICC patients and performed immunohistochemistry to detect α-smooth muscle actin (α-SMA), CD3, CD4, CD8, Foxp3, CD68, and CD66b.
Background: The tumor microbiome has been characterized in several malignancies; however, no previous studies have investigated its role in intrahepatic cholangiocarcinoma (ICC). Hence, we explored the tumor microbiome and its association with prognosis in ICC.
Methods: One hundred and twenty-one ICC tumor samples and 89 adjacent normal tissues were profiled by 16S rRNA sequencing.
Article Synopsis
- BRAF variants, particularly the V600E subtype, are linked to tumor characteristics and prognosis in intrahepatic cholangiocarcinoma (ICC), but their overall prevalence and effects on treatment response are not well understood.
- This study analyzed 1,175 ICC patients who underwent surgery between 2009 and 2017 in China, using sequencing methods to identify BRAF variants and assess their impact on patient survival rates.
- Results showed that BRAF V600E variants were associated with larger tumor sizes and multiple tumors, indicating that specific BRAF subtypes may influence disease progression and treatment outcomes in ICC patients.
Article Synopsis
- KRAS variants, particularly certain subtypes, are linked to the progression and poorer survival outcomes in patients with intrahepatic cholangiocarcinoma (ICC) after surgical treatment.
- A study conducted on 1024 ICC patients identified 14 subtypes of KRAS variants, with G12D being the most common, occurring in 43.3% of the cases with KRAS mutations.
- The results showed that patients with KRAS variants had higher levels of specific tumor markers, and the G12 variants were specifically associated with worse overall survival compared to patients without KRAS mutations.
Article Synopsis
- Intrahepatic cholangiocarcinoma (ICC) is a highly aggressive cancer with a tendency to relapse after surgery, making long-term survival rates low and highlighting the need for more understanding of its genomic changes during relapse.
- Researchers performed whole-exome sequencing on paired primary and relapsed tumors from patients to identify genetic alterations and analyze how these changes affect tumor behavior and growth, particularly focusing on a gene called SLIT2.
- The study found that relapsed tumors often developed new mutations while retaining key drivers, and specific mutations in SLIT2 were linked to tumor progression and immune cell interactions, suggesting that SLIT2 plays a significant role in ICC's aggressive nature and metastatic potential.
Background: Long noncoding RNAs (lncRNAs) are functionally associated with cancer development and progression. Although gene copy number variation (CNV) is common in hepatocellular carcinoma (HCC), it is not known how CNV in lncRNAs affects HCC progression and recurrence. We aimed to identify a CNV-related lncRNA involved in HCC progression and recurrence and illustrate its underlying mechanisms and prognostic value.
View Article and Find Full Text PDFSemaphorin 3B (SEMA-3B), which belongs to the semaphorin family, has an important role in cell apoptosis and inhibition of angiogenesis. A previous study by our group revealed that SEMA-3B was downregulated in tumor tissues of patients with hepatocellular carcinoma (HCC) and exerts anti-motility and anti-invasion effects on tumor cells. However, the serum levels of SEMA-3B and their clinical significance have remained elusive; therefore, the aim of the present study was to monitor its expression in HCC and investigate its clinical significance.
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