Single-cell and spatial transcriptomics reveal 5-methylcytosine RNA methylation regulators immunologically reprograms tumor microenvironment characterizations, immunotherapy response and precision treatment of clear cell renal cell carcinoma.

Clear cell Renal Cell Carcinoma (ccRCC) is a highly heterogeneous disease, making it challenging to predict prognosis and therapy efficacy. In this study, we aimed to explore the role of 5-methylcytosine (m5C) RNA modification in ccRCC and its potential as a predictor for therapy response and overall survival (OS). We established a novel 5-methylcytosine RNA modification-related gene index (M5CRMRGI) and studied its effect on the tumor microenvironment (TME) using single-cell sequencing data for in-depth analysis, and verified it using spatial sequencing data.

Identification of mRNA vaccines and conserved ferroptosis related immune landscape for individual precision treatment in bladder cancer.

Background: The aim of this study was to identify the ferroptosis induced tumor microenvironment (FeME) landscape in bladder cancer (BCa) for mRNA vaccine development and selecting suitable patients for precision treatment.

Methods: Gene expression profiles and clinical information of 1216 BCa patients were extracted from TCGA-BLCA, three GEO databases and IMvigor210 cohort. We comprehensively established the FeME landscape of 1216 BCa samples based on 290 ferroptosis related genes (FRGs), and systematically correlated these regulation patterns with TME cell-infiltrating characteristics.

The performance of transrectal ultrasound in the diagnosis of seminal vesicle defects: a comparison with magnetic resonance imaging.

Obstructive azoospermia (OA) is one of the most common causes of male infertility. Transrectal ultrasound (TRUS) has been used to diagnose OA for many years. From 2009 to 2013, we evaluated a prospective cohort of 1249 patients with suspected OA using TRUS.

[Transrectal ultrasonography in the etiological diagnosis of male obstructive azoospermia: analysis of 695 cases].

Objective: To assess the role of transrectal ultrasonography (TRUS) in the etiological diagnosis of male obstructive azoospermia.

Methods: We retrospectively analyzed the clinical data and TRUS findings of 695 patients with obstructive azoospermia from January 2007 to May 2009.

Results: Concerning the etiology of obstructive azoospermia, the main TRUS findings included ejaculatory duct abnormality (29.

Study on the effects of allitride and its mechanisms on renal cell carcinoma in vitro.

Objective: To study the killing effect of allitride on human renal cell carcinoma cell line Ketr-3 and its possible mechanisms.

Methods: The Ketr-3 cells were treated with allitride and the morphological changes were observed with inverted microscope. The cytotoxicity was estimated through theamine blue tetrazolium bromide (MTT).

[Value of transrectal ultrasonography in the diagnosis of midline prostatic cysts].

Objective: To assess the value of transrectal ultrasonography (TRUS) in the diagnosis of midline prostatic cysts.

Methods: We retrospectively analyzed the TRUS manifestations of 87 cases of midline prostatic cysts.

Results: Of the total number, 33 cases were diagnosed as Müllerian duct cysts, 21 cases ejaculatory duct cysts and the other 33 cases undifferentiated midline prostatic cysts; 19 cases had dilated seminal vesicles, 19 seminal vesicle agenesis, 9 seminal vesiculitis and 5 dilation of the ejaculatory duct.

[Individualization of transrectal ultrasonography-guided prostate biopsy for prostate cancer detection].

Background & Objective: Transrectal ultrasonography (TRUS)-guided sextant biopsy technique was regarded as golden standard method for the diagnosis of prostate cancer. Recently, many reports show that the detection rate of prostate cancer by sextant biopsy is not high, and suggest to take more cores to improve the detection rate. But there is no ideal protocol now.

[Sonographic features and clinical significance of transrectal ultrasonography in obstructive azoospermia].

Objective: To investigate the ultrasonographic features of obstructive azoospermia and to evaluate transrectal ultrasonography in the diagnosis of the disease.

Methods: We performed transrectal ultrasonography for 248 patients with obstructive azoospermia, observed the sonographic features of the prostate gland, seminal vesicle and ejaculatory duct.

Results: The average volume of the prostate gland of the studied group was 13.

[Surgical therapy for azoospermia with ejaculatory duct obstruction].

Objective: To investigate the effect of transurethral resection of ejaculatory ducts (TURED) for azoospermia with ejaculatory duct obstruction (EDO).

Methods: From June 2003 to December 2004, 20 azoospermia with EDO were diagnosed, diagnostic criteria included a history, physical examination, semen analyses, semen fructose measurement, endocrine assessment, testicular biopsy and transrectal ultrasonography (TRUS); All 20 cases were treated by TURED. Fifteen of them were followed up more than 3 months after the treatment.

[Diagnosis and treatment of renal cell carcinoma with vena cava tumor thrombi].

Background & Objective: Renal cell carcinoma (RCC) might involve the renal vein, and form tumor thrombus extending into the vena cava or the right atrium. Treating RCC with vena cava involvement is difficult in clinical practice. Radical nephrectomy with complete tumor thrombus removal could result in good outcomes for RCC patients with vena cava thrombi.