Publications by authors named "Rong-Mei Peng"
Cytokine analysis of aqueous humor in patients with cytomegalovirus corneal endotheliitis.
Graefes Arch Clin Exp Ophthalmol
August 2024
Purpose: To evaluate cytokine levels of aqueous humor in patients with cytomegalovirus (CMV) corneal endotheliitis and their relationships with CMV DNA load.
Methods: 44 aqueous humor samples were obtained from 26 patients with CMV corneal endotheliitis at various stages of treatment. 33 samples obtained from cataract patients during the same period were selected as a control group.
Purpose: The purpose of this study was to investigate the morphologic features of the cornea in patients with unilateral posterior interstitial keratitis.
Methods: Seven eyes of 7 patients with unilateral posterior interstitial keratitis were examined by slit-lamp biomicroscopy, anterior segment optical coherence tomography (AS-OCT), and confocal microscopy (IVCM). The imaging features of the cornea were evaluated and analyzed.
Aim: To investigate corneal graft survival rate and endothelial cell density (ECD) loss after keratoplasty in cytomegalovirus (CMV) positive patients.
Methods: This was a retrospective cohort study. We analyzed the clinical data of patients who underwent viral DNA detection in aqueous humor/corneal tissue collected during keratoplasty from March 2015 to December 2018 at the Peking University Third Hospital, Beijing, China.
Purpose: To evaluate the clinical outcomes of penetrating keratoplasty (PK) and Descemet's stripping automated endothelial keratoplasty (DSAEK) in eyes with irreversible corneal decompensation secondary to Axenfeld-Rieger syndrome (ARS).
Methods: In this retrospective case series, a total of four eyes undergoing PK and seven eyes undergoing DSAEK, including one eye requiring one repeat DSAEK, between 2014 and 2021 were enrolled. Postoperative complications, graft survival, glaucoma treatment before and after keratoplasty, visual outcomes, and endothelial cell density were recorded.
Purpose: To develop a fully automated segmentation and morphometric parameter estimation system for assessing corneal endothelial cells from in vivo confocal microscopy images.
Design: Artificial intelligence (neural network) study.
Methods: First, a fully automated deep learning system for assessing corneal endothelial cells was developed using the development set (from 99 subjects).
Purpose: The purpose of this study was to analyze tear cytokine and complement levels in patients diagnosed with acute ocular graft-versus-host disease (oGVHD) and examine the consistency of these levels with the severity of clinical manifestations.
Methods: Ten patients with acute oGVHD (20 eyes) were enrolled for the assessment of tear cytokine levels and ocular surface parameters, and 18 healthy people (36 eyes) were selected as the control group. The tear cytokine and complement levels were measured using microsphere-based immunoassay analysis.
Purpose: To report the clinical manifestations, postoperative complications and long-term outcomes of endothelial keratoplasty in VZV-related endothelial decompensation.
Methods: In this retrospective study, thirteen eyes undergoing endothelial keratoplasty (EK) for VZV-related endothelial decompensation were compared with controls for Fuchs endothelial dystrophy or pseudophakic bullous keratopathy.
Results: Twelve patients did not have typical dermal pain or blisters.
Purpose: The goal was to develop a fully automated grading system for the evaluation of punctate epithelial erosions (PEEs) using deep neural networks.
Methods: A fully automated system was developed to detect corneal position and grade staining severity given a corneal fluorescein staining image. The fully automated pipeline consists of the following three steps: a corneal segmentation model extracts corneal area; five image patches are cropped from the staining image based on the five subregions of extracted cornea; a staining grading model predicts a score for each image patch from 0 to 3, and automated grading score for the whole cornea is obtained from 0 to 15.
Purposes: To understand the pathogenesis in rat corneal endothelial cells (RCECs) induced by murine cytomegalovirus infection .
Methods: , cultured RCECs were infected with murine cytomegalovirus strain K181-eGFP (MCMV-eGFP). , experimental rats received intracameral injection of MCMV-eGFP.
The incidence and influence of the donor corneas positive for herpesviridae DNA in keratoplasty.
Graefes Arch Clin Exp Ophthalmol
December 2020
Purpose: We detected the DNA of herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in donor corneas and assessed the clinical outcomes of recipients who received virus-positive grafts.
Method: All donor corneas were analyzed for the presence of HSV-1, HSV-2, VZV, CMV, and EBV by real-time PCR from April 2017 to July 2019. The medical records of the transplant patients who received virus-positive grafts were reviewed.
Background: To compare endothelial loss between recipients who received viral DNA-positive grafts and controls 2 years after corneal transplantation.
Methods: We retrospectively analysed the clinical data and endothelial cell density of recipients of viral DNA-positive grafts and age-, sex-, aetiology- and operation-matched controls from April 2017 to July 2019 at the Peking University Third Hospital, Beijing, China.
Results: A total of 23/942 (2.
Purpose: To identify the feasibility of reconstructing corneal endothelial sheets by seeding non-infected monoclonal human corneal endothelial cells (HCECs) onto porcine Descemet's membrane (DM) and verifying the function in vitro and in vivo.
Methods: Denuded porcine DM was decellularized for haematoxylin and eosin staining, and DNA was removed via incubation with ethylene glycol diglycidyl ether (EGDE). The physical properties of the incubated DMs were evaluated and compared to those of unincubated DMs.
Background: There is still controversy over the necessity of screening donor corneas for herpes simplex virus (HSV). Currently, no study reported the outcomes of different types of keratoplasty with HSV-positive donor corneas.
Objectives: To describe the clinical consequences of four patients who underwent keratoplasty by sharing double corneas from a single donor, both of which were positive for HSV-1 DNA by polymerase chain reaction.
Characteristics of Corneal Endotheliitis among Different Viruses by in Vivo Confocal Microscopy.
Ocul Immunol Inflamm
February 2021
: To explore the cellular morphological characteristics and changes in corneal endotheliitis among different viruses by in vivo confocal microscopy (IVCM).: Corneal confocal images of 44 eyes of 44 patients with HSV, VZV, CMV and EBV corneal endotheliitis were studied retrospectively. Corneal confocal images of 44 normal eyes were used as controls.
View Article and Find Full Text PDFPurpose: To evaluate the safety and pharmacokinetic changes of ganciclovir (GCV) intraocular injection.
Methods: GCV (2 mg/0.1 mL) was injected into rabbit eyes.
Clinical Evaluation of Intravitreal Injection of Ganciclovir in Refractory Corneal Endotheliitis.
Ocul Immunol Inflamm
January 2021
: To evaluate the efficacy and safety of intravitreal ganciclovir (GCV) injection in refractory endotheliitis.: Retrospectively recruited 25 eyes with endotheliitis, proved by clinical manifestations, positive PCR for viral DNA and responded poor to topical and systemic antiviral medications. All patients received additional continued intravitreal GCV injections.
View Article and Find Full Text PDF: To explore the clinical characteristics and confocal microscopic (IVCM) findings of varicella zoster virus (VZV)-related corneal endotheliitis.: Retrospectively reviewed 20 eyes with corneal edema which were diagnosed by real-time polymerase chain reaction.: one had VZV infection.
View Article and Find Full Text PDFAim: To evaluate the complications and outcomes of descemet stripping automated endothelial keratoplasty (DSAEK) combined with artisan aphakia intraocular lens (IOL) implantation in severely damaged eyes without capsular support.
Methods: DSAEK combined with artisan iris claw IOL implantation was performed on 29 eyes. All eyes were of abnormal structure due to complications from prior intraocular surgeries and ocular trauma.
Importance: There is limited literature on paediatric donors in endothelial keratoplasty.
Background: This study investigated the efficacy of and appropriate paediatric donor age for Descemet's stripping endothelial keratoplasty (DSEK).
Design: Retrospective and observational case series.
Background: Bone marrow mesenchymal stem cells (BM-MSCs) are multipotential stem cells that have been used for a broad spectrum of indications. Several investigations have used BM-MSCs to promote photoreceptor survival and suggested that BM-MSCs are a potential source of cell replacement therapy for some forms of retinal degeneration.
Purpose: To investigate the expression of the MER proto-oncogene, involved in the disruption of RPE phagocytosis and the onset of autosomal recessive retinitis pigmentosa in rat BM-MSCs and to compare phagocytosis of the photoreceptor outer segment (POS) by BM-MSCs and RPE cells in vitro.
Objective: To evaluate the influence of corneal thickness (CT) on the intraocular pressure (IOP) measurements, the agreement of IOP readings with I Care rebound tonometer (RBT) and Goldmann applanation tonometer (GAT), and the agreement of central and peripheral IOP values obtained by RBT.
Methods: Thirty-seven eyes of 34 patients after Descemet's stripping with automated endothelium keratoplasty (DSAEK) underwent CT measurement using anterior segment OCT (AS-OCT) followed by IOP evaluation with the GAT at corneal center and with the RBT at corneal center and at 1 mm from the limbus in the nasal and temporal directions. The mean IOP measurement by the RBT and measurement by the GAT were analysed by paired t test.
Purpose: To investigate the outcomes and complications of suture pull-through insertion techniques for Descemet stripping automated endothelial keratoplasty (DSAEK) in Chinese phakic eyes.
Patients And Methods: Retrospective case series. Included in the study were all Chinese patients with phakic eyes who underwent DSAEK at Peking University Third Hospital from August 2008 to August 2011.
Objective: To evaluate the effect and explore the complications of Descemet-stripping automated endothelial keratoplasty (DSAEK) combined with phacoemulsification cataract surgery or lens exchange in corneal endothelial dysfunction eyes with lens disorders.
Methods: Retrospective case series. Eighteen consecutive cases (20 eyes) were performed DSAEK combined with lens surgery from December 2007 to December 2008 in Department of Ophthalmology, Peking University Third Hospital.
Purpose: To explore the feasibility of reattaching the dislocated graft with viscoelastic in abnormally structured eyes after Descemet-stripping automated endothelial keratoplasty (DSAEK).
Design: Retrospective case series.
Participants: Five pseudophakic bullous keratopathy cases (5 eyes) with a history of vitrectomy and/or iris-lens diaphragm injury.