Synaptic connections between endomorphin 2-immunoreactive terminals and μ-opioid receptor-expressing neurons in the sacral parasympathetic nucleus of the rat.

The urinary bladder is innervated by parasympathetic preganglionic neurons (PPNs) that express μ-opioid receptors (MOR) in the sacral parasympathetic nucleus (SPN) at lumbosacral segments L6-S1. The SPN also contains endomorphin 2 (EM2)-immunoreactive (IR) fibers and terminals. EM2 is the endogenous ligand of MOR.

[Expression of COX10 in human non-obstructive azoospermia testes].

Objective: To evaluate the expression of COX10 mRNA in the testes of non-obstructive azoospermia patients and normal men.

Methods: A cDNA microarray containing COX10 and some other genes as RBM and EIF1AY was used to identify the differential gene expression profiles in the normal and azoospermic testes. The cDNA probes were prepared by labeling mRNA from azoospermic and normal testis tissues with Cy5-dUTP and Cy3-dUTP respectively through reverse transcription.

[The biological characteristics of beta glucuronidase cDNA transfected renal cancer cell line GRC 1/betaG].

Aim: To establish a renal carcinoma cell line which can highly express beta-glucuronidase(betaG), and to observe the biological characteristics of the transfected cells.

Methods: Recombinant eukaryotic expression vector pcDNA3.1-betaG was constructed.

[Clinical investigation of renal angiomyolipoma].

Objective: To study the diagnosis and management of renal angiomyolipoma (RAML), and to identify risk factors affecting spontaneous angiomyolipoma rupture.

Methods: The data of 68 patients with RAML from 1989 to 2002 were retrospectively reviewed. These patients were divided in two groups on the basis of tumor size, 35 patients in group A ( 4 cm).

Expression and significance of Rap1A in testes of azoospermic subjects.

Aim: To evaluate the Rap1A mRNA expression and its significance in the testes of normal and azoospermic subjects.

Methods: A cDNA microarray that contained Rap1A and some other genes such as RBM, EIF1AY was used to identify the differential gene expression profiles between the normal and azoospermic testes. cDNA probes were prepared by labeling mRNA from azoospermic and normal testicular tissues through reverse transcription with Cy5-dUTP and Cy3-dUTP, respectively.

[Diagnosis and treatment of seminal vesicle cyst].

Objectives: To deepen the understanding of patients with seminal vesicle cyst for correct diagnosis and treatment.

Methods: Sixteen patients with seminal vesicle cysts were treated in the period of January 1980-May 2002. Their symptoms, diagnostic results, treatment and outcomes were analyzed retrospectively.

[The relationships of PSA, FPSAR, clinical and pathological stage in patients with prostate cancer].

Objectives: To investigate the relationship between clinical and pathological stage, serum prostate specific antigen (PSA) concentration and free-to-total PSA ratio (FPSAR) in patients with prostate cancer.

Methods: Clinical and pathological stage were determined on the basis of pathological examination and clinic material in 42 prostate cancer patients treated by prostatectomy. PSA and FPSAR were measured before the operation.