MiR-153-5p promotes sensibility of colorectal cancer cells to oxaliplatin via targeting Bcl-2-mediated autophagy pathway.

Oxaliplatin (L-OHP) is one of the effective chemotherapeutic drugs for colorectal cancer (CRC). Further investigation into the molecular mechanism of chemoresistance could improve outcomes for patients with colorectal cancer. Recently, microRNAs have been reported as a key in drug resistance of tumors.

Beta-cyclodextrin-centered star-shaped amphiphilic polymers for doxorubicin delivery.

Aim: Delivery of doxorubicin could be achieved by a novel micellar system based on beta-cyclodextrin-centered star-shaped amphiphilic polymers (sPEL/CD). This study specifically explored the effect of polylactide segments in sPEL/CD on various micelle properties, such as the critical micelle concentration, size, drug loading, cytotoxicity and drug resistance reversing effect.

Method: The sPEL/CD was synthesized by the arm-first method.

[Control of shortcut nitrification in SBBR with adequate oxygen supply].

At the high level of dissolved oxygen (DO) in sequencing batch biofilm reactor (SBBR), the approach and mechanism for realizing shortcut nitrification were researched. Landfill leachate was used as handling of object, the mainly environment parameters of the reactor were controlled as follow: DO 5 mg/L, pH 7.0, temperature 25 degrees C, adopted all drainage mode and 12-hour cycle influent.

[Flocculating capability and mechanism of bioflocculant produced by Paenibacillus polymyxa GA1].

MBFGA1, which was produced by Paenibacillus polymyxa GA1, was used to investigate its flocculating capability in the following waste water, the liquid of kaolin, soil suspended liquid, coal-washing wastewater and the landfill leachate with orthogonal experiments, the inspection of granularity and the scanning electron microanalysis. Meanwhile the anthrone reaction and infrared spectrum are also applied to identify its main component. In the optimal flocculating conditions obtained from the orthogonal experiments, the flocculating rates of the liquid of kaolin, soil suspended liquid, coal-washing wastewater and the landfill leachate were 99.

[Approaches of biological nitrogen removal in a single sequencing batch biofilm reactor].

The conventional microorganism techniques and the molecule biological techniques such as PCR and DGGE were utilized to study the approaches of biological nitrogen removal in a single sequencing batch biofilm reactor (SBBR). The main approach of biological nitrogen removal, no less than 65% of the total NH4(+)-N was removed in this approach, was composed of partial nitrification, anaerobic ammonium oxidation and denitrification. The second approach included twain processes such as partial nitrification and denitrification, and the third one was conventional nitrogen removal process (nitrification and denitrification).

[Bacterial diversity in sequencing batch biofilm reactor (SBBR) for landfill leachate treatment using PCR-DGGE].

For studying the bacterial diversity and the mechanism of denitrification in sequencing bath biofilm reactor (SBBR) treating landfill leachate to provide microbial evidence for technique improvements, total microbial DNA was extracted from samples which were collected from natural landfill leachate and biofilm of a SBBR that could efficiently remove NH4+ -N and COD of high concentration. 16S rDNA fragments were amplified from the total DNA successfully using a pair of universal bacterial 16S rDNA primer, GC341F and 907R, and then were used for denaturing gradient gel electrophoresis (DGGE) analysis. The bands in the gel were analyzed by statistical methods and excided from the gel for sequencing, and the sequences were used for homology analysis and then two phylogenetic trees were constructed using DNAStar software.