Differential protein expression in EC304 gastric cancer cells induced by alphastatin.

Objective: To explore the differential protein expression profile in EC304 gastric cancer cells induced by alphastatin.

Methods: Cultured EC304 cells in the exponential phase of growth were randomly divided into alphastatin and control groups. Total proteins were extracted and the two dimensional electrophoresis (2-DE) technique was applied to analyze differences in expression with ImageMaster 2D Platinum 5.

[Regulation of proliferation and apoptosis of human vascular endothelial cell by Acheron].

Objective: To investigate regulatory effect of Acheron (Achn) on proliferation and apoptosis of human vascular endothelial cell.

Methods: (1) Eahy926 cells were cultured in serum-free DMEM medium (96-well plates) and were divided into Achn inhibition group (transfected with plasmid psi-Achn), psi4.1 group (transfected with psi4.

[Effect of early intensive insulin therapy on high mobility group box 1 protein levels and prognosis of patients with severe trauma].

Objective: To study the effect of intensive insulin therapy on serum high mobility group box 1 (HMGB1) levels and its relationship with the prognosis in early phase of severe trauma.

Methods: Eighty severe trauma patients [injury severity score (ISS)≥ 16] were divided into groups according to injury to matched anatomical regions. Forty patients of intensive therapy group were given early intensive insulin therapy, while another 40 patients of the conventional treatment group received routine treatment based on clinical experience with insulin treatment.

[Inhibition of integrin beta1 expression in human keratinocyte stem cells by vector-1 based interfering RNA strategy].

Objective: To investigate the influence of integrin beta1 on the proliferation and differentiation of human keratinocyte stem cells (KSCs).

Methods: DNA oligonucleotides targeting integrin beta1 at different locations were synthesized and inserted into BamHI-1 HindIII linearized p Silencer 3.1/H1 plasmids.

[Purification of human endothelial overexpressed lipopolysaccharide-associated factor 1 protein].

Objective: To investigate the feasibility of obtaining of a highly pure protein of human endothelial overexpressed lipopolysaccharide-associated factor 1 (EOLA1) with metal chelation chromatography.

Methods: Inclusion bodies of the E. coli transformed with EOLA1 gene were extracted and washed with BugBuster Protein Extraction Reagent.