Publications by authors named "Rong-Hua Cao"
Objective: To investigate the characteristics and predictors for anastomotic leakage after oesophagectomy for oesophageal carcinoma from the perspective of anastomotic level.
Design: Retrospective cohort study.
Settings: A single tertiary medical centre in China.
Objective: To evaluate whether RNA interference can protect porcine endothelial cells from complement mediated cytotoxicity.
Methods: Immortalized porcine aortic endothelial cells of the line PED were cultured and transfected with alpha1,3-galactosyltransferase (alpha1, 3-GT) specific siRNAs. Cells transfected with mismatch SiRNA was used as negative controls.
Inhibition of xenogeneic response in porcine endothelium using RNA interference.
Transplantation
February 2005
Article Synopsis
- The study focuses on using small interfering RNA (siRNA) to silence the alpha1,3 galactosyltransferase (alpha1,3GT) enzyme in porcine cells to reduce the alpha-Gal epitope, aiming to decrease immune rejection in xenotransplantation.
- Successful siRNA transfection led to a significant reduction in alpha1,3GT mRNA and alpha-Gal expression, confirmed through various assays, including Western blotting and flow cytometry.
- The silenced porcine endothelial cells showed protection from human serum-mediated cytotoxicity, suggesting that siRNA could be a promising strategy for addressing immune rejection in organ transplants.