[Construction of yeast one-hybrid library and screening of transcription factors regulating LS expression in Ganoderma lucidum].

Lanosterol synthase( LS) is a key enzyme involving in the mevalonate pathway( MVA pathway) to produce lanosterol,which is a precursor of ganoderma triterpenoid. And the transcriptional regulation of LS gene directly affects the content of triterpenes in Ganoderma lucidum. In order to study the transcriptional regulation mechanism of LS gene,yeast one-hybrid technique was used to screen the transcription regulators which interact withthe promoter of LS.

The influence of C2C3 double bond on the antiradical activity of flavonoid: Different mechanisms analysis.

Flavonoids widely found in bee products are excellent antioxidants. The structural features are important in evaluating the antiradical activity of flavonoid. In this work, the density functional theory (DFT) methods were applied to investigate the influence of C2C3 double bond on the antiradical activity of flavonoid based on three prevalently accepted radical scavenging mechanisms from the thermodynamic aspect.

Antioxidant Activity of Quercetin and Its Glucosides from Propolis: A Theoretical Study.

Among the multiple components of propolis, flavonoids contribute greatly to the antioxidant activities of propolis. Flavonoids mainly exist in the form of sugar-conjugated derivatives. Quercetin glycosides represent the predominant flavonoid fraction in propolis.

Hydrogen-bonding Interactions between Apigenin and Ethanol/Water: A Theoretical Study.

In this work, hydrogen-bonding interactions between apigenin and water/ethanol were investigated from a theoretical perspective using quantum chemical calculations. Two conformations of apigenin molecule were considered in this work. The following results were found.

[Effects of different culture conditions on main chemical compositions of Anoectochilus roxburghii].

Objective: To investigate the effects of different culture conditions on the main chemical compositions of Anoectochilus roxburghii, so as to determine the optimum culture conditions and provide theoretical support for large-scale production of Anoectochilus roxburghii.

Methods: The light qualities, photoperiods and induction periods were changed to regulate the main chemical compositions of Anoectochilus roxburghii.

Results: The contents of total flavonoids, quercetin, isorhamnetin and kaempferol in blue light were higher than that in yellow light, the worst was under red light.