Proteomics analyses of activated human optic nerve head lamina cribrosa cells following biomechanical strain.

Purpose: To determine protein regulation following activation of human, optic nerve head (ONH), lamina cribrosa (LC) cells in response to mechanical strain.

Methods: LC cells were isolated and grown from donor tissue in specific media at 37°C and 5% CO(2) humidified incubator. Cells were grown to confluence on collagen I-coated flexible-bottom culture plates, rinsed with Dulbecco's phosphate-buffered saline, and left for 24 hours in serum-free media.

Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.

We investigate the role of glial cell activation in the human optic nerve caused by raised intraocular pressure, and their potential role in the development of glaucomatous optic neuropathy. To do this we present a proteomics study of the response of cultured, optic nerve head astrocytes to biomechanical strain, the magnitude and mode of strain based on previously published quantitative models. In this case, astrocytes were subjected to 3 and 12% stretches for either 2 h or 24 h.

In vitro contact angle analysis and physical properties of blister pack solutions of daily disposable contact lenses.

Objectives: The purpose of this study was to measure the advancing and receding contact angles (CAs) of five daily disposable (DD) lenses and the osmolality, surface tension (ST), and pH of each blister pack solution.

Methods: The advancing and receding CAs were measured directly out of the blister pack for five DD lenses: omafilcon A (CooperVision), nelfilcon A (CIBA Vision), modified nelfilcon A (CIBA Vision), etafilcon A (Johnson & Johnson), and narafilcon A (Johnson & Johnson). Advancing CAs were measured using sessile drop and Wilhelmy balance methods.

Physical properties of soft contact lens solutions.

Purpose: To investigate the physical properties of commercially available soft contact lens solutions.

Methods: The pH, osmolality, surface tension (ST), and viscosity of various soft contact lens solutions were measured at room temperature. Viscosity measurements were also taken at 34 degrees C.

The impact of lipid on contact angle wettability.

Purpose: To analyze the effect of in vitro lipid doping on conventional hydrogel (CH) and silicone hydrogel (SH) lens wettability, assessed by sessile drop contact angle (CA) measurement.

Methods: Nine contact lens materials, five SHs and four CH, were incubated with two different lipid tear solutions (LTS) containing cholesterol, cholesteryl oleate, oleic acid, oleic acid methyl ester, and triolein. The first LTS was a "low" concentration solution, which was close to human values, and the second was a "high" concentration.