Publications by authors named "Ronan Cristhian Teixeira"
Selection and validation of reference genes for RT-qPCR gene expression studies in Candida viswanathii cultivated under different grown conditions.
Matheus Martins Daúde Ronan Cristhian Teixeira Carlos Henrique Cardon Gessi Carvalho de Araujo Santos Alex Fernando de Almeida
J Microbiol Methods
August 2023
Article Synopsis
- Candida viswanathii produces lipases with potential applications in various industries, including food and pharmaceuticals, but research on its molecular growth processes is limited.
- The study focused on identifying suitable reference genes for RT-qPCR experiments in C. viswanathii using different carbon sources, analyzing eleven candidate genes for their expression stability.
- Results indicated that CvACT and CvRPB2 are the best reference gene pair overall, with specific pairs being optimal when carbon sources like olive oil and glucose are considered individually, aiding future gene expression studies in this species.
Isolating high quality RNA is a limiting factor in molecular analysis, since it is the base for transcriptional studies. The RNA extraction method can directly affect the RNA quality and quantity, as well as, its overall cost. The industrial importance of the yeast genus Candida in several sectors comes from their capacity to produce Lipases.
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