Peroxide release into saliva from five different home bleaching systems in vivo.

Purpose: This study determined hydrogen peroxide release into the oral cavity during use of different home bleaching products and compared them with accepted safe levels.

Methods: Determination of peroxide in saliva was performed with peroxidase, phenol and 4-aminoantipyrin in a photometric method. Upper jaw incisors were bleached with individual trays charged with 350 mg Opalescence 10%, Opalescence 15% (OP) and Vivastyle (V).

Immobilisation and activity of human alpha-amylase in the acquired enamel pellicle.

Unlabelled: Amylase is an important salivary component and structural element of the acquired enamel pellicle. Aim of the study was to establish a method for precise and direct determination of pellicle bound amylase activity in order to analyse kinetics and activity of the immobilised enzyme. Six bovine enamel slabs (5mm diameter) were fixed on individual maxillary trays and worn by five subjects for different times (3, 30 and 120 min) on buccal and palatal sites on different days.

Determination of peroxides in saliva--kinetics of peroxide release into saliva during home-bleaching with Whitestrips and Vivastyle.

Aim of the study was to determine peroxides in saliva, released during bleaching procedures. Upper incisors of five subjects were bleached with Whitestrips (5% H2O2) and Vivastyle (10% carbamide peroxide, tray charged with 225mg) for 30min, each on different days. Saliva was collected before and during the whole period of bleaching at different intervals.