[Intestinal bacterial colonisation and translocation in C3H/HeJ conventional mice fed a unique dose of live or dead Bifidobacterium breve C50].

The efficiency and safety of use of Bifidobacterium breve C50 (BbC50), a potential probiotic, was assessed as regards intestinal microbial colonisation and bacterial translocation. A suspension of BbC50, containing 1-5 to 107-108 live bacteria, was fed to C3H/HeJ mice. The passage of live BbC50 was not demonstrated by culture either in the intestine or extra-intestinal organs.

Partial characterization of bifidobacterium breve C50 cell-free whey compounds inducing modifications to the intestinal microflora.

Cell-free whey obtained from milk fermented with Bifidobacterium breve C50 (Bb C50) has been shown to modify the intestinal flora in humans and mice. Previous work showed that no antibiotic-like or barrier effect due to overgrowing bifidobacteria was implied in the microbial modifications. The present study was focused on characterizing the compounds and mechanisms involved.

Bacterial microleakage of Cavit, IRM, TERM, and Fermit: a 21-day in vitro study.

The aim of our study was to evaluate the leakage of four cements (Cavit, IRM, TERM, and Fermit) using a two-compartment model system and Streptococcus sanguis as bacterial marker. Access cavities in premolars were filled with cement and the teeth immersed in culture medium in the model system. Half of the teeth were thermocycled on day 2.

Cell-free whey from milk fermented with Bifidobacterium breve C50 used to modify the colonic microflora of healthy subjects.

The ingestion of viable bacteria is thought to be required to modify intestinal microflora. In the present study, the effects on fecal flora of consumption of cell-free concentrated whey from milk that had been fermented with Bifidobacterium breve C50 was tested using 10 healthy human volunteers. Results were compared with effects of a commercial milk formula that had been fermented with Streptococcus thermophilus and B.

Cell-free wheys from bifidobacteria fermented milks exert a regulatory effect on the intestinal microflora of mice and humans.

Bacteroides fragilis and clostridia are normally present in the human colon but they may exert pathogenic effects when the homeostasis is upset following various forms of stress. One approach to preventing gastrointestinal disorders is to use bifidobacteria fermented milk. It has been suggested that the efficacy of such a product is related to abiotic compounds produced during milk fermentation.

Bifidobacteria and human health: regulatory effect of indigenous bifidobacteria on Escherichia coli intestinal colonization.

Bifidobacteria are assumed to exert colonization resistance to enteric pathogens. We associated C3H germfree mice with either Bifidobacterium longum or Escherichia coli or both strains and studied how they settled in the gut and the lymphoid organs as well as their effect on mucus composition. Within 24 hE.

Bacterial microleakage of Cavit, IRM, and TERM.

In this in vitro study, a model system was developed and tested to evaluate the sealing ability of temporary restorative materials used in endodontic access preparations. The materials studied, Cavit, IRM, and TERM, were tested on 40 premolars against a known bacterial species, Streptococcus sanguis. The leakage of bacterial cells was checked 4 and 8 days after initial immersion in the culture.

Regulation of the bacterial intestinal implantation in infant born by caesarean section.

Studies were undertaken to determine the regulation of the bacterial intestinal implantation in 19 newborns delivered by caesarean section. Correlation was made with the infant feeding mode. The effect of human milk seemed to be the result of B.

In vitro effects of titanium powder on oral bacteria.

Biomaterial research has been mostly concerned with biocompatibility, i.e. tissue response, of materials for dental or orthopaedic implantation.

Treatment of diversion colitis by short-chain fatty acids. Prospective and double-blind study.

Diminished production of short-chain fatty acids (SCFA) by altered flora has been suggested in the pathogenesis of diversion colitis (DC). We evaluated prospectively the effectiveness of SCFA irrigation in 13 patients with excluded colon (eight males, five females; mean age, 48 years). The causes of diversion were inflammatory bowel disease (n = 4), colonic cancer (n = 2), sigmoid diverticulitis with perforation (n = 3), ischiorectal abscess (n = 2), and miscellaneous (n = 2).

Phenotypic differentiation of bifidobacteria of human and animal origins.

The phenotypes of 153 strains belonging or related to the genus Bifidobacterium were studied. These organisms included 38 collection strains and 115 wild strains (41 strains of human origin, 56 strains of animal origin, and 18 strains obtained from rivers or sewage). Our phenotypic analysis revealed seven main groups that were subdivided into 20 subgroups.

Survival of Prevotella intermedia (Bacteroides intermedius) in transport media.

Five transport media were selected for testing, in vitro, the survival of a pure strain of Prevotella intermedia (Bacteroides intermedius) for 6, 24, and 72 hours. Two were non-nutrient transport media (RTF and VMG IV). The three others (TG, PY, PYG) were nutrient media.

[Behavior of Clostridium perfringens in the intestine of newborn infants born by cesarean section].

Colonization of the digestive tract by C. perfringens was studied in infants born by cesarean section. Correlations between the level of colonization and the environment, type of feeding, and presence of other anaerobic bacteria were looked for.

Bacterial interactions in the intestine of the newborn delivered by cesarean section.

The purpose of this study was to clarify the role of the intestinal anaerobic bacteria colonizing the intestine of the newborn delivered by cesarean section. Control of the intestinal microecology is dependent on many factors including intestinal peristalsis, the intraluminal environment, and microbial interactions, that deter the overgrowth of pathogens populations. Numerous factors help achieve this normal balance.

Nosocomial infections of ocular conjunctiva in newborns delivered by cesarian section.

Colonization of the ocular conjunctiva in newborns delivered by cesarian section occurs usually within the first day of life. We have studied the flora of the ocular conjunctiva at birth, from 19 newborns delivered by cesarian section, coming from two different maternity hospitals. Ocular conjunctiva cultures yielded the main predominant flora in both maternity hospitals considered.

Gram(+) anaerobic intestinal cocci in healthy adults and neonates.

Anaerobic gram(+) cocci were found to be part of the normal indigenous flora of the intestinal tract in healthy adults (19.6%) but they were rarely isolated from healthy neonates (6.1%).

In vitro survival of Bacteroides intermedius in five transport media, alone or in the presence of Streptococcus sanguis.

The survival rate of Bacteroides intermedius was first tested in monoculture, and Streptococcus sanguis was then added in 5 different transport media; 2 nonnutritious media, the viability-preserving medium of the University of Göteborg No. IV (VMG IV), reduced transport fluid (RTF), and 3 nutritious media, thioglycolate medium (TG), peptone yeast extract medium (PY) and PY medium with 1% glucose (PYG). All manipulations were carried out in an anaerobic chamber.

A collaborative study of French laboratories to assess any evolution of antibiotic resistance within the Bacteroides fragilis group.

Antibiotic susceptibility testing of anaerobes by a same methodology allows the authors to draw up suggestions about the evolution of antibiotic resistance within the B. fragilis group. Cefoxitin resistance rates were stable until 1985 and were slowly increasing later.

[Detection of beta-lactamases in the Bacteroides fragilis group].

One hundred and thirty three strains of Bacteroides fragilis group isolated from clinical specimen were tested for beta-lactamase activity against five beta-lactam antibiotics, ampicillin, cefaloridin, cefuroxime, cefotaxime and cefoxitin. The Minimal Inhibitory Concentration of antibiotics was determined using the agar dilution method. Beta-lactamase production was detected by a microbiological method in 128 of the 133 (96%) strains.

[Host-Bifidobacterium interactions in the axenic mouse: partial characterization of bifidogenic factors in the intestinal contents].

Growth factors for Bifidobacterium bifidum were detected in faeces of axenic mice strain C3H. Most of these factors were found in the nondialyzable fraction obtained after aqueous extraction and dialysis. SDS-PAGE and filtration chromatography on Sepharose 4B revealed that many glycosylated components harbored a bifidigenic activity.

Effect of the feeding practices on the establishment of bacterial interactions in the intestine of the newborn delivered by cesarean section.

The purpose of this study was to clarify the role of the intestinal anaerobe bacteria colonizing the intestine of the newborn delivered by cesarean section. The intestinal bacterial colonization of 18 newborns delivered by cesarean section was assessed sequentially over the first 14 days of life. Emphasis is placed on C.

Occurrence of Enterobacteriaceae and E. coli in the intestine of the newborn by caesarean section.

It has been established that colonization of the gastrointestinal tract by Enterobacteriaceae and especially E. coli in newborn delivered by caesarean section occurs generally within the second day of life. On the 14th day, these bacteria inhabit the fecal flora of the majority of newborn.

Apparition of Clostridium sp. and Bacteroides in the intestine of the newborn delivered by cesarian section.

Anaerobic flora plays a key role in preventing intestinal colonization with potential pathogens. Nowadays, the mechanisms involved in the colonization resistance provided by the anaerobic microflora are to be clarified. Numerous factors seem to intervene in the regulation of the intestinal flora.

Occurrence of Bifidobacterium in the feces of newborns delivered by cesarean section.

In cesarean section newborns, colonization by Bifidobacterium occurs generally within 4 days of life. Breast-fed infants, independent of the delivery procedure, harbor a gastrointestinal flora characterized by a predominance of Bifidobacterium. Breast-fed [corrected] newborns delivered by cesarean section present a predominance of Bifidobacterium bifidum and bifidobacterium infantis in their stools.