Morphological changes in adrenal cortex--the final effect of angiotensin II receptor antagonists.

The influence of losartan, angiotensin receptor on the histological image of the adrenal glomerular layer of white rats was revealed. The drug was administered in the therapeutic and ten times higher dose. It was manifested by the decrease of glandular cells and prevalence of blastic cells of the cortex.

The proximal convoluted tubule of rats' nephron after experimental administration of gentamicin.

The rats in this experiment received gentamicin in intraperitoneal injections in a dose ten times bigger than therapeutical. After ten days of drug administration, in the cells of proximal convoluted tubules of kidney there were observed the dilution of cytoplasm of various intensity, increases in amount and size of lysosomes, widening of endoplasmic reticulum tubules and swelling of mitochondria. The microscopic and ultrastructural pictures, after three weeks' break in gentamicin administration, showed fast regeneration of renal tubules.

Ultrastructure of the hepatocytes after application of azithromycin (Sumamed).

Azithromycin (Sumamed, PLIVA Cracow) was administered intragastrically to rats in doses 10 times and 100 times bigger than the therapeutic dose (according to the indications for a 5-day treatment). Ultrastructural examinations of the liver were performed. Changes observed in the morphology of hepatocytes and Browicz-Kupffer cells (swelling of mitochondria, more numerous lysosomes, weaker RER content and granular material inside biliary canaliculi) may indicate temporary violation of the functional equilibrium in the liver due to the application of azithromycin.

Histological picture of intestinal mucosa of rats after simultaneous administration of atorvastatin and ethyl alcohol.

The rats received atorvastatin in the therapeutical dose and 10x higher, 20% solution of ethanol and ethanol with atorvastatin for 6 weeks. The microscopic investigations (H + E staining, Masson's method, PAS method by McManus) showed no changes in histological picture of intestinal mucosa after giving atorvastatin. The rats receiving alcohol and alcohol with atorvastatin showed similar changes, but different than the first groups.

Does ACE inhibitor modify the morphofunctional state of the nephron?

Captopril, the inhibitor of the angiotensin convertase (ACE) was administered to white rats in two doses: 0.23 mg/day/rat and 0.71 mg/day/rat.

[Examination of gastric juice in animals fed with fodder containing power plant ashes and loess dusts].

Normal metabolism, regulation of stimulation and checking of electrolyte secretion in the gastric juice depend on the way of feeding and its nutritional ingredients. Feeding experimental animals with fodder containing power plant ashes or loess dusts causes a change in the gastric juice reaction, thus resulting in a different assimilation of elements contained in the fodder. There was shown an increase of some elements content (Mg, Na, Cu, Fe), a decrease in the content of some others (Pb,Zn) in the gastric juice and a positive tendency for blocking by calcium ions of heavy metals contained in the fodder by the mucous membrane of the stomach.

[Study of rat behavior in a feeding experiment].

The examination studied behaviour of rats fed on natritive material containing soil dust or electroenergetic ashes. Food containing the dust or ashes was administered to animals in the amount of 30 g a day for 26 weeks. Body weight was controlled in all animals and their motor activity, motor coordination and cognitive activity were examined.

[Histochemical and histologic examination of kidney from white rats after experimental administration of dexamethasone].

Kidneys of the rats which had been intragastrically administered Dexamethasone (10 mg/kg), were examined. Observations were carried out after a single and twice repeated application of the preparation, as well as after 7 days of everyday administration. It was found, on the basis of histological and histochemical observations (reaction to alkaline phosphatase activity, reaction to acid phosphatase activity, PAS-method staining), that both single and twice administration of Dexamethasone do not cause the damage of the kidney parenchyma, whereas after 7 days of everyday application of the preparation irreversible changes can be observed.

[Histoenzymatic picture of rat gastric mucosa under the influence of dexamethasone].

White male [correction of female] rats were given Dexamethasone in a single dose 0.35 mg/24 hrs. Group I was given the drug once, group II--received it for 2 days, and group III--for 7 days.

[Morphological evaluation and analysis of trace elements in the liver of rats who were fed with feed containing power station ashes].

The experiment was carried out on white Wistar rats. The rats in the experimental groups received fodder containing power station ashes for various periods of time (from 6 to 18 weeks). During the experiment, the body weight of rats was recorded, the amount of trace elements in the liver was estimated, and morphological tests of the liver were carried out.

[Effect of diet containing soil dust on morphological examination of liver in experimental rats].

Investigations refer to the influence of fodder containing soil dust as one of the ingredients on morphology of the liver and the weight of experimental animals. Fodder containing soil dust was given to animals for various periods of time, the longest was 18 weeks. The results indicate that the intensity of morphological changes in the liver depends on the time of giving fodder.

[Cytoenzymatic studies on the influence of soil dust on the cornea and conjunctiva of experimental animals].

The studies of the effect of the soil dust on the cornea and the conjunctiva of the experimental animals have been performed. The rats were divided into 3 experimental groups and 1 control group. The animals were given 3 different, selected at random, kinds of soil dust to the conjunctival sac 3 times daily for 7 days.

[Statistical analysis of body and lung mass of animals subjected to a single experimental insufflation of soil dust and electro-energetic ashes].

The studies were performed on 44 white female Wistar rats which were intratracheally administered the suspension of the soil dust and the electro-energetic ashes. The electro-energetic ashes were collected from 6 different local heat and power generating plants while the soil dust from several random places of our country. The statistical analysis of the body and the lung mass of the animals subjected to the single dust and ash insufflation was performed.

[Histological studies and clinical observations of the effect of various types of dust on the organ of vision of small laboratory animals].

For seven days, three times a day, female rats were instilled into their conjunctival sacs suspensions of power plant dusts, soil and clay. Clinically observed were inflammatory changes of the conjunctiva and cyclids margins as well as traumatisation of the corneal and conjunctival epithelia, appearing at different stages on the experiment. Cytoenzymatic examinations indicated disturbances of oxidation and reduction processes, changes in dialysis through physiologic membranes and dissociation of protein synthesis.

[Histochemical studies of rat lungs after the short-term influence of soil dust].

There was examined the biological activity of soil dusts using histochemical methods. Intratracheal administration of dusts used in this study is a common method for testing activity of industrial dusts. The used soil dusts were characterized by high content of free silicon dioxide, 3 times higher than its content in dusts from power stations released in the process of burning coal and approximately to the amount in graphite dust.

[Determining acid phosphatase (AcP[E.C. 3.1.3.2.]) and adenosine triphosphatase (ATPase [E.C. 3.6.1.3.]) activity in the lungs of rats following a single administration of ashes from industrial high-heat facilities].

There was examined pulmonary tissue of white rats, which had been administered intratrachealy a single dose of the respirable fraction of ashes sample from 6 different power stations elektrohasting plants and hasting plants in Poland (0.2 ml suspension; 50 mg of the examined sample in 0.6 cm3 of NaCl solution).

[Soil dust as a factor which changes the histochemical picture of the rat lung].

White Wistar female rats only once were administered intratracheally a suspension of the soil dust in the physiologic saline. The respirable fraction of the soil dust was used for preparing the suspension of the latter. The experiment was carried out in 2 groups of rats.

[Effect of short-term exposure to ash from electric power plants on histochemical reactions of succinate dehydrogenase and lactate dehydrogenase in the lungs of experimental animals].

Histochemical studies of the activity of succinic dehydrogenase (SDH) and lactic dehydrogenase (LDH) in the pulmonary tissue of experimental animals which were given four coal ashes issued from different thermal-electric power stations: group VII--from the thermal-electric power station Siekierki (Warsaw), group VIII--from Wrocław, group IX--from Leg (Cracow) and group XI--from Lódź, were carried out. In particular ash samples the content of trace elements and free silica was determined. The studies yield the following conclusions: 1.

[Behavior of selected oxidoreductases in histochemical and electro- phoretic studies of the rat kidney after administration of Biseptol 480 (co-trimoxazole)].

Examinations of the rat kidney metabolism were carried out by histological (staining with haematoxylin and eosin), histochemical (response to lactate and succinic dehydrogenases activities), and electrophoretic methods (separation of isoenzymes of lactate dehydrogenase) after treating animals with the sulphonamide Biseptol 480, for 7 (consecutive) d by stomach tube. Biseptol given to the animals exceeded 10 times human therapeutical dose. It was found out that Biseptol 480, applied to the animals with healthy urinary system, does not pathologically disturb the examined stages of the metabolic processes in the rat's kidney.