Transcriptomic analysis of synchrony and productivity in self-cycling fermentation of engineered yeast producing shikimic acid.

Industrial fermentation provides a wide variety of bioproducts, such as food, biofuels and pharmaceuticals. Self-cycling fermentation (SCF), an advanced automated semi-continuous fermentation approach, has shown significant advantages over batch reactors (BR); including cell synchrony and improved production. Here, engineered to overproduce shikimic acid was grown under SCF operation.

Temperature, pH, and Glucose Responsive Gels via Simple Mixing of Boroxole- and Glyco-Based Polymers.

Statistical copolymers of -isopropylacrylamide (NIPAAm) and 5-methacrylamido-1,2-benzoxaborole (MAAmBo) have been synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. The solution properties of the NIPAAm homopolymers and statistical copolymers were investigated and it was found that, besides temperature and pH, the statistical copolymers were also responsive to the presence of free glucose in solution. Furthermore, responsive hydrogels and nanogels were formed spontaneously by simply mixing the statistical copolymers of P(NIPAAm--MAAmBO)s and well-defined glycopolymers.