Microbial Biofilms: Features of Formation and Potential for Use in Bioelectrochemical Devices.

Microbial biofilms present one of the most widespread forms of life on Earth. The formation of microbial communities on various surfaces presents a major challenge in a variety of fields, including medicine, the food industry, shipping, etc. At the same time, this process can also be used for the benefit of humans-in bioremediation, wastewater treatment, and various biotechnological processes.

Targeted Formation of Biofilms on the Surface of Graphite Electrodes as an Effective Approach to the Development of Biosensors for Early Warning Systems.

Biofilms based on bacteria () and () and yeast () were used for novel biosensor creation for rapid biochemical oxygen demand (BOD) monitoring. Based on the electrochemical measurement results, it was shown that the endogenous mediator in the matrix of and biofilms and ferrocene form a two-mediator system that improves electron transport in the system. Biofilms based on and had a high biotechnological potential for BOD assessment; bioreceptors based on such biofilms had high sensitivity (the lower limits of detectable BOD concentrations were 0.

Bioanalytical System for Determining the Phenol Index Based on BS394(pBS216) Bacteria Immobilized in a Redox-Active Biocompatible Composite Polymer "Bovine Serum Albumin-Ferrocene-Carbon Nanotubes".

The possibility of using the microorganisms sp. 7p-81, BS394(pBS216), s67, 5Ap, X5, F5 and DSM 11331 as the basis of a biosensor for the phenol index to assess water environments was studied. The adaptation of microorganisms to phenol during growth was carried out to increase the selectivity of the analytical system.

Electroactive Biofilms of Activated Sludge Microorganisms on a Nanostructured Surface as the Basis for a Highly Sensitive Biochemical Oxygen Demand Biosensor.

The possibility of the developing a biochemical oxygen demand (BOD) biosensor based on electroactive biofilms of activated sludge grown on the surface of a graphite-paste electrode modified with carbon nanotubes was studied. A complex of microscopic methods controlled biofilm formation: optical microscopy with phase contrast, scanning electron microscopy, and laser confocal microscopy. The features of charge transfer in the obtained electroactive biofilms were studied using the methods of cyclic voltammetry and electrochemical impedance spectroscopy.