Publications by authors named "Roman A Zingarelli"
Because of limits on specificity and purity to allow for in-depth protein profiling, a standardized method for exosome isolation has yet to be established. In this study, we describe a novel, in-house microfluidic-based device to isolate exosomes from culture media and patient samples. This technology overcomes contamination issues because sample separation is based on the expression of highly specific surface markers CD63 and EpCAM.
View Article and Find Full Text PDFBackground: Genomic studies have revealed that multiple genes are mutated at varying frequency in endometrial cancer (EC); however, the relevance of many of these mutations is poorly understood. An EC-specific recurrent mutation in the MAX transcription factor p.His28Arg was recently discovered.
View Article and Find Full Text PDFArticle Synopsis
- STAT3 is found to be over-expressed in type 1 endometrial cancer, and the study explores how glucose levels affect its expression and the impact of metformin as a potential treatment.
- In experiments with Ishikawa cancer cells, high glucose concentrations increased STAT3 and its target proteins, while metformin treatment reduced cell growth and increased apoptosis, leading to lower levels of STAT3 proteins.
- The findings suggest that high glucose promotes STAT3 expression in type 1 endometrial cancer, but metformin can inhibit this effect, showing promise as a therapeutic option.