Publications by authors named "Romain Juge"

Acne is an inflammatory skin disease of the pilosebaceous unit, involving four essential factors: hyperseborrhoea combined to a modification of sebum composition, colonization by Cutibacterium (C.) acnes, hyperkeratinization and secreted inflammation. Understanding and mimicking compromised skin is essential to further develop appropriate therapeutic solutions.

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Melanoma is a highly metastatic and deadly form of cancer. Invasive melanoma cells overexpress integrin αβ, which is a well-known target for Arg-Gly-Asp-based (RGD) peptides. We developed a sophisticated method to synthetize milligram amounts of a targeted vector that allows the RGD-mediated targeting, internalization, and release of a mitochondria-disruptive peptide derived from the pro-apoptotic Bax protein.

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GA101, also known as obinutuzumab or Gazyva (Gazyvaro), is a glycoengineered type II humanized antibody that targets the CD20 antigen expressed at the surface of B-cells. This novel anti-CD20 antibody is currently assessed in clinical trials with promising results as a single agent or as part of therapeutic combinations for the treatment of B-cell malignancies. Detailed understanding of the mechanisms of GA101-induced cell death is needed to get insight into possible resistance mechanisms occurring in patients.

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UV irradiation is a major environmental factor causing skin dryness, aging and cancer. UVB in particular triggers cumulative DNA damage, oxidative stress and mitochondrial dysfunction. The objective of our study was to provide both qualitative and quantitative analysis of how mitochondria respond to UVB irradiation in normal human epidermal keratinocytes (NHEK) of healthy donors, with the rationale that monitoring mitochondrial shape will give an indication of cell population fitness and enable the screening of bioactive agents with UVB-protective properties.

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Anti-apoptotic Bfl-1 and pro-apoptotic Bax, two members of the Bcl-2 family sharing a similar structural fold, are classically viewed as antagonist regulators of apoptosis. However, both proteins were reported to be death inducers following cleavage by the cysteine protease µ-calpain. Here we demonstrate that calpain-mediated cleavage of full-length Bfl-1 induces the release of C-terminal membrane active α-helices that are responsible for its conversion into a pro-apoptotic factor.

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Synopsis of recent research by authors named "Romain Juge"

  • - Romain Juge's research primarily focuses on the mechanisms of skin conditions, particularly acne, and the impact of environmental factors like UV radiation on skin health, as evidenced by his work developing a 3D in vitro acne model and quantifying UVB-induced mitochondrial fragmentation in keratinocytes.
  • - He has explored targeted cancer therapies, particularly for melanoma, developing innovative methods for the systemic delivery of tumor-targeted peptides while investigating the molecular mechanisms of anti-cancer antibodies, such as the unexpected cross-reactivity of anti-cathepsin B antibodies in the context of CD20-targeting treatments.
  • - Juge's findings underscore the intricate relationships between apoptosis regulation, mitochondrial function, and skin pathology, revealing novel insights into both therapeutic development and the fundamental biological processes affecting skin and cancer cells.

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