Publications by authors named "Romain Bouchat"
Thermobacillus xylanilyticus is a thermophilic and hemicellulolytic bacterium of interest for the production of thermostable hemicellulases. Enzymes' production by this bacterium is challenging, because the proliferation of a cheating subpopulation of cells during exponential growth impairs the production of xylanase after serial cultivations. Accordingly, a strategy of successive cultivations with cells transfers in stationary phase and the use of wheat bran and wheat straw as carbon sources were tested.
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- The study investigates the production of hemicellulolytic enzymes by the bacterium Thermobacillus xylanilyticus, which produces a key enzyme (Tx-xyn11) but suffers from variability in enzyme production among individual cells.
- Experiments showed that after 23.5 generations of culturing on xylan, there was a significant decline (about 75%) in xylanase activity and gene expression, linked to shifts in cellular populations with differing growth characteristics.
- Further investigations indicated that a specific subpopulation of bacterial cells, which did not sporulate, had lower enzyme activity and a longer lag phase, highlighting the importance of understanding cell diversity for improving enzyme production.