New prodrugs of acyclic nucleosides with antiviral activity.

More than 130 acyclic purine and pyrimidine nucleoside derivatives have been synthesized. Structure-activity relationships among a selected group of 6- and 9-substituted guanine derivatives will be discussed. By introduction of secondary aliphatic (e.

Fluorescent, DNA-binding dyes for rapid detection of Chlamydia trachomatis.

The newer DNA-binding fluorochromes DAPI (4',6-diamidino-2-phenylindole) and fluorochrome 33258 H (Hoechst) (2-[2-(4-hydroxy-phenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)-benzimidazole . 3 HCl) proved useful in identifying genital strains of Chlamydia trachomatis in McCoy cells. For practical purposes, e.

Optical demonstration of Chlamydiae in cell cultures by means of fluorochrome 33,258 H.

Fluorochrome 33,258 H, a bisbenzimidazole, was employed for demonstrating Chlamydiae in cell cultures, and proved to be particularly suitable for illustrating the unique intracellular reproduction processes of these organisms. The staining procedure is simple and permits a selective differentiation of chlamydial inclusions and their developmental processes in the cell. Contrary to other staining methods, all stages of the chlamydial reproduction cycle can be illustrated thus permitting the early demonstration of Chlamydiae in the cell.

[Chemotherapeutic effects of nitro compounds. 1. Nitroanilines].

More than 200 nitro compounds, most of them nitroaniline derivatives substituted with one or more radicals having a basic reaction, were prepared and investigated as to their therapeutic activity against bacteria, fungi, protozoa, helminths, viruses and tumors. Several mono-nitrobenzenes with a radical having a basic reaction showed weak in-vitro activity against gram-positive bacteria and against Crocker's sarcoma 180; they also showed systemic activity against nematodes (Aspiculuris tetraptera) and viruses. The majority of therapeutically active compounds with pronounced in-vivo activity against Trichomonas fetus, Entamoeba histolytica, Schistosoma mansoni, cestodes, nematodes (Ancylostoma caninum), viruses (influenza, MHV, SAV and EMC) and various types of carcinoma (Ehrlich's carcinoma, leukemia 1210, Crocker's sarcoma 180) were dinitrobenzene derivatives with one radical having a basic reaction and electropositive groups or unreactive or reactive chlorine atom, and di-nitrobenzene with two equal or two different radicals having a basic reaction.

Inhibition of release of vaccinia virus by N1-isonicotinoly-N2-3-methyl-4-chlorobenzoylhydrazine.

N(1)-isonicotinoyl-N(2)-3-methyl-4-chlorobenzoylhydrazine (IMCBH) is a selective inhibitor of vaccinia virus multiplication. In concentrations up to 50 microg/ml, IMCBH causes neither toxic morphologic changes, nor does it inhibit the multiplication of cells. Viruses other than vaccinia are not affected by IMCBH.