Publications by authors named "Rolf Schauder"
Production of a novel heterodimeric two-chain insulin-Fc fusion protein.
Protein Eng Des Sel
September 2020
Insulin is a peptide hormone produced by the pancreas. The physiological role of insulin is the regulation of glucose metabolism. Under certain pathological conditions the insulin levels can be reduced leading to the metabolic disorder diabetes mellitus (DM).
View Article and Find Full Text PDFArticle Synopsis
- The drug discovery process begins with creating plasmids that code for specific proteins, aided by advanced cloning techniques that enable the generation of thousands of plasmids easily.
- Recent advancements in miniaturization have allowed for the parallel expression and purification of many proteins using robotic systems, although this typically results in low yields of purified protein suitable only for sensitive assays.
- To tackle the need for larger quantities and higher purity, an automated NGC chromatography system has been employed, facilitating a three-step purification process that significantly reduces production time and increases efficiency.