Long-Term Storage and Longevity of Orthodox Seeds: A Systematic Review.

As part of conservation of plant genetic resources, long-term storage of seeds is highly relevant for genebanks. Here we present a systematic review and a meta-analysis of studies on seed longevity focusing on half-life (P) under different storage conditions. Six studies were selected for the meta-analysis; in addition, a high number of additional references were included in the discussion of the results.

Seed Germination after 30 Years Storage in Permafrost.

More than 30 years ago, the Nordic Gene Bank established a long-term experiment on seeds stored under permafrost conditions in an abandoned mine corridor in Svalbard, as a tool to monitor storage life under these conditions. The study included seeds from 16 Nordic agricultural and horticultural crops, each represented by two or three cultivars (altogether 38 accessions). All seeds were ultra-dried to 3%-5% moisture before being sealed in glass tubes.

Genetic diversity and population structure of leafy kale and Brassica rupestris Raf. in south Italy.

Local varieties of leafy kales (Brassica oleracea L.) are grown in home gardens in Calabria and Sicily for self-consumption, in the same area where the wild relative Brassica rupestris Raf. also grows.

Connection between rod bivalents and incomplete meiotic association at NORs in Hordeum marinum Huds.

The hypothesis of a connection between rod bivalent formation and incomplete meiotic association at NORs of SAT-chromosomes of H. marinum is supported. PMCs of H.

Identifying the genome of wood barley Hordelymus europaeus (Poaceae: Triticeae).

Wood barley, Hordelymus europaeus, was compared with other Triticeae species by Southern and fluorescence in situ hybridisation using total genomic DNA and repetitive sequences as probes. On Southern blots, the total genomic probe from H. europaeus hybridised strongly to DNA of its own species and to Leymus and Psathyrostachys, indicating the presence of Ns genome in H.

Salt tolerance in wild Hordeum species is associated with restricted entry of Na+ and Cl- into the shoots.

Eight wild Hordeum species: H. bogdanii, H. intercedens, H.

Ancestry of American polyploid Hordeum species with the I genome inferred from 5S and 18S-25S rDNA.

Background And Aims: The genus Hordeum exists at three ploidy levels (2x, 4x and 6x) and presents excellent material for investigating the patterns of polyploid evolution in plants. Here the aim was to clarify the ancestry of American polyploid species with the I genome. *

Methods: Chromosomal locations of 5S and 18S-25S ribosomal RNA genes were determined by fluorescence in situ hybridization (FISH).

Screening and evaluation of resistance to downy mildew (Peronospora parasitica) and clubroot (Plasmodiophora brassicae) in genetic resources of Brassica oleracea.

52 entries including landraces, old cultivars and wild accessions of B. oleracea and closely related Brassica species were screened for resistance against downy mildew and clubroot. Several accessions resistant to downy mildew and a few to clubroot were found.

Inter simple sequence repeat analysis of genetic diversity and relationships in cultivated barley of Nordic and Baltic origin.

This study evaluates putative changes of genetic diversity and relationships of barley in the Nordic and Baltic countries that might have taken place during the last century as a result of commercial breeding. Four ISSR primers were used to analyse 227 accessions, yielding a total of 47 polymorphic loci. Shannon-Weaver diversity values for each locus ranged from 0.

Diversity in root aeration traits associated with waterlogging tolerance in the genus Hordeum.

Growth, root aerenchyma, and profiles of radial O loss (ROL) along adventitious roots were evaluated in 35 'wild' Hordeum accessions and cultivated barley (H. vulgare L. ssp.

The genome composition of hexaploid Psammopyrum athericum and octoploid Psammopyrum pungens (Poaceae: Triticeae).

The genomic constitution of two species in the genus Psammopyrum, i.e., Ps.

Genetic variation in three species of Hordeum, and the selection of accessions for the Barley Core Collection.

Starch gel electrophoresis of isozymes was used to investigate the level and distribution of genetic variation in accessions of three wild, diploid Hordeum species. Allelic variation was assessed for seven isozyme loci and used in the statistical analysis of 18 accessions of Hordeum brachyantherum subsp. californicum, 29 accessions of H.

Molecular phylogeny of the genus Hordeum using three chloroplast DNA sequences.

The genus Hordeum consists of three cytotypes (2x, 4x, and 6x). Its reproductive isolation has been incomplete between closely related species and hence the genetic relationship is reticulate and complex. We used 32 taxa of Hordeum and three chloroplast DNA sequences, matK, atpB-rbcL, and trnL-trnF in the current study.

Characterization of genetic diversity in core collection accessions of wild barley, Hordeum vulgare ssp. spontaneum.

Genetic variability in the 143 core accessions of wild barley, Hordeum vulgare ssp. spontaneum, was assessed by allozyme analysis. A total of 34 alleles were detected at ten isozyme loci.

Genetic diversity amongst landraces of rye (Secale cereale L.) from northern Europe.

Genetic variation and diversity were estimated for three improved varieties and 18 landraces of Secale cereale, originating from the northern Europe. This material was compared with eight improved varieties and 16 landraces from Sweden, which were analysed before. The analysis used starch gel electrophoresis with the enzymes ACO, GPI, MDH, PGD and PGM and resulted in one fixed locus and seven polymorphic loci.