Development and validation of a ready to use cryo-EROD assay for the standardized screening of dioxins and dioxin-like compounds in foodstuffs.

Recent European regulations have indicated the need for new bioanalytical screening methods capable of monitoring dioxin and dioxin-like compounds in foodstuffs and environmental samples, cost-effectively and with a quicker turnaround. Cryo-cells of the hepatic H4IIE line preserved in 96-well plates were exposed to sample extracts prepared from various foodstuffs and analysed for their content of dioxins and dioxin-like compounds by means of the 7-Ethoxyresorufin-O-Deethylase (EROD)-assay in two laboratories. Assay data were compared between both laboratories and results from instrumental analysis used as a confirmatory method.

Impact of perfluorooctanesulfonate and perfluorooctanoic acid on human peripheral leukocytes.

Perfluorinated compounds (PFCs), such as perfluorooctanesulfonate (PFOS) and perfluorooctanoic acid (PFOA), are xenobiotics that can be detected worldwide in the environment, wildlife, and humans. So far, the immunotoxicity of PFCs has only been investigated in rodents, but not in humans. In this study, we explore the impact of PFOS and PFOA on selected functions of human leukocytes in vitro.

Bolus ingestion but not regular consumption of native or dealcoholized red wine modulates selected immunological functions of leukocytes in healthy volunteers.

Background/aims: Red wine (RW) consumption reduces the risk for coronary heart disease (CHD). Since immunological mechanisms involved in CHD were modulated by RW polyphenols in vitro, we investigated whether consumption of native or dealcoholized RW (DRW) affects selected immunological parameters in healthy adults ex vivo.

Methods: Twenty-seven nonsmokers were randomized to consume a single dose of 200 ml RW, 175 ml DRW or 200 ml water (controls).

Skeletal deformations in medaka (Oryzias latipes) visualized by synchrotron radiation micro-computer tomography (SRmicroCT).

Synchrotron radiation micro-computer tomography (SRmicroCT) offers the possibility to investigate biomineralized structures in high detail. Two animals of adult medaka fish (Oryzias latipes) were analyzed by SRmicroCT with a resolution of 6.55 microm: the wild-type animal was normally developed whereas the second animal showed an idiopathic deformation of the cranial and axial skeleton.

Inflammatory cytokines induce protein tyrosine nitration in rat astrocytes.

Protein tyrosine nitration may be relevant for the pathogenesis of hepatic encephalopathy (HE). Infections, sepsis, and trauma precipitate HE episodes. Recently, serum levels of tumor necrosis factor (TNF)-alpha were shown to correlate with severity of HE in chronic liver failure.

Dynamic expression of sparc precedes formation of skeletal elements in the Medaka (Oryzias latipes).

Sparc is a secreted calcium-binding glycoprotein that regulates mineralization of bone tissues in mammals. In other vertebrates, its function remains largely unclear. Here, we describe the isolation, genomic organization and expression of the sparc gene in the teleost Medaka (Oryzias latipes), an established vertebrate model for developmental studies.

Single and repeated moderate consumption of native or dealcoholized red wine show different effects on antioxidant parameters in blood and DNA strand breaks in peripheral leukocytes in healthy volunteers: a randomized controlled trial (ISRCTN68505294).

Background: Red wine (RW) is rich in antioxidant polyphenols that might protect from oxidative stress related diseases, such as cardiovascular disease and cancer. Antioxidant effects after single ingestion of RW or dealcoholized RW (DRW) have been observed in several studies, but results after regular consumption are contradictory. Thus, we examined if single or repeated consumption of moderate amounts of RW or DRW exert antioxidant activity in vivo.

Antigen-specific targeting and elimination of EBV-transformed B cells by allergen toxins.

Background: With the exception of antigen-specific immunotherapy, current treatments for atopic diseases provide only symptomatic relief. Because of the increasing incidence of such diseases, the development of novel strategies and concepts for the treatment of allergies is urgently needed.

Objective: Here we present a new approach for the treatment of atopic diseases.

Modulation of endothelial and smooth muscle function by bed rest and hypoenergetic, low-fat nutrition.

Prolonged microgravity alters the regulation of the peripheral vasculature. The influence of reduced food intake, as often observed in astronauts, on vascular function is unclear. In a randomized, four-phase, crossover study, the effect of simulated microgravity (13 days of bed rest), energetic restriction (-25%, fat reduced), and their combination on endothelium-dependent and -independent vasodilation was compared with ambulatory control conditions.

A proinflammatory role for Fas in joints of mice with collagen-induced arthritis.

Collagen-induced arthritis (CIA) is a chronic inflammatory disease bearing all the hallmarks of rheumatoid arthritis, e.g. polyarthritis, synovitis, and subsequent cartilage/bone erosions.