Publications by authors named "Roland Ebert"
On the biosynthesis of specialized pro-resolving mediators in human neutrophils and the influence of cell integrity.
Biochim Biophys Acta Mol Cell Biol Lipids
March 2022
Neutrophils are key players in inflammation initiation and resolution. Little attention has been paid to the detailed biosynthesis of specialized pro-resolving mediators (SPM) in these cells. We investigated SPM formation in human polymorphonuclear leukocytes (PMNL), in broken PMNL preparations and recombinant human 5-lipoxygenase (5-LO) supplemented with the SPM precursor lipids 15-Hydroxyeicosatetraenoic acid (15-HETE), 18-Hydroxyeicosapentaenoic acid (18-HEPE) or 17-Hydroxydocosahexaenoic acid (17-HDHA).
View Article and Find Full Text PDFDesigned polypharmacology presents as an attractive strategy to increase therapeutic efficacy in multi-factorial diseases by a directed modulation of multiple involved targets with a single molecule. Such an approach appears particularly suitable in non-alcoholic steatohepatitis (NASH) which involves hepatic steatosis, inflammation and fibrosis as pathological hallmarks. Among various potential pharmacodynamic mechanisms, activation of the farnesoid X receptor (FXRa) and inhibition of leukotriene A4 hydrolase (LTA4Hi) hold promise to counteract NASH according to preclinical and clinical observations.
View Article and Find Full Text PDFToll-like receptor signaling induces a temporal switch towards a resolving lipid profile in monocyte-derived macrophages.
Biochim Biophys Acta Mol Cell Biol Lipids
September 2020
Inflammation is a tightly regulated process. During the past decade it has become clear that the resolution of inflammation is an active process and its dysregulation can contribute to chronic inflammation. Several cells and soluble mediators, including lipid mediators, regulate the course of inflammation and its resolution.
View Article and Find Full Text PDFLong-term stimulation of toll-like receptor-2 and -4 upregulates 5-LO and 15-LO-2 expression thereby inducing a lipid mediator shift in human monocyte-derived macrophages.
Biochim Biophys Acta Mol Cell Biol Lipids
September 2020
Article Synopsis
- Macrophages can change their function (polarization) during inflammation, influenced by lipid signals released from cells.
- The study examined how human macrophages, derived from blood monocytes, develop into either M1 or M2 types under specific conditions and respond to various pathogen stimuli.
- Results indicated that M1 macrophages primarily produce pro-inflammatory signals, while sustained stimulation of M2 macrophages leads to the production of anti-inflammatory and pro-resolving lipid mediators, indicating a shift in their activity.