Evaluation of angiogenesis in delayed skin flaps using a monoclonal antibody for the vascular endothelium.

The angiogenic process in delayed and undelayed flaps was evaluated in relation to different periods of delay (6 h, 72 h and 7 days) and at different times after the definitive elevation of the flaps (48 h, 96 h and 7 days). An immunohistochemical method has been tested to stain the endothelial cells of capillaries and venules, providing a high specificity for labelling these vessels. No signs of angiogenesis were found in undelayed and 6-h delayed flaps at 48 h but there was an increase of capillaries from 48 h to 7 days after elevation of the flaps.

Restrictive cardiomyopathy caused by chloroquine.

A 59 year old white woman who had been treated with chloroquine phosphate for 25 years presented with signs of congestive heart failure and was diagnosed as having restrictive cardiomyopathy by non-invasive methods. Electron microscopy of a biopsy specimen of skeletal muscle showed lesions compatible with chloroquine myopathy. The patient died five weeks after presentation.

Electrocardiographic patterns in V4R lead in experimental right ventricular infarction of rats.

Twenty-four male rats that became hypertensive after complete ligature of the abdominal aorta, just above the origin of the left renal artery, showed combined myocardial infarction. To study the electrocardiographic patterns of the experimental right ventricular infarction, the V4R right thoracic lead was recorded at different time intervals after aortic ligature. The patterns recorded were as follows: Q waves in 23 cases (95.

Morphological changes in the vascularisation of delayed flaps in rabbits.

The effect of the delay phenomenon on the vascularisation of the pectoralis major myocutaneous flap was studied in 32 rabbits. In 36 flaps, submitted to different methods of delay, vascularisation was studied by making the tissue transparent and comparing the results with a control group of 16 undelayed flaps. In another 12 flaps, 6 of them previously delayed, the vascularisation of the skin was studied by light microscopy and through a morphometric analysis (image digitalizing process).

[Biochemical plasma changes in essential hypertension patients treated for a year].

The possibility that certain antihypertensive drugs produce metabolic alterations in patients with hypertension treated during one year, has been prospectively assessed in 40 patients with essential hypertension with matched age and blood pressure readings. Four groups (Gr) of 10 patients each were studied. Group 1 received 100 mg/day of atenolol (AT), group 2 received 5 mg of amiloride and 50 mg of hydrochlorothiazide (4 tablets per week), group 3 received 40 mg/day of nifedipine retard (NF) and group 4 received 50 mg/day of captopril (CP).

Predominant right ventricular infarction in malignant renovascular hypertension in rats.

Malignant hypertension was induced in Wistar rats of both sexes by complete aortic ligature just above the origin of the left renal artery. An acute and a late phase of hypertension, 4 days and 28 days after the aortic ligature respectively, were defined to study the relationship between the severity of hypertension and injury of the small arteries and myocardial necrosis. The most frequent finding in the heart was the presence of right ventricular infarction.

[Reflex pressure response to the electric stimulation of different parts of the rat stomach].

The role of the autonomic nervous system in the pressor response to the electrical stimulation of different gastric zones has been studied in rats. The stimulus was applied before and after the following interventions: bilateral vagotomy, ganglionic blockade, alpha-adrenergic receptor blockade and beta-adrenergic receptor blockade. After the ganglionic blockade no pressor responses to the electrical stimulus were observed.

Reflex pressor response to gastric mechanical stimulation in rats.

Neural and humoral mechanisms involved in the reflex pressor response during mechanical stimulation of the stomach of rats were investigated. The arterial blood pressure response was prevented by inhibition of alpha-adrenergic vasoconstriction using either an alpha-adrenergic blocker or a ganglionic blocker. In addition, there was a small decrease in the response after nephrectomy.

Reduced ethanol consumption during cyproheptadine administration in rats from a long-term alcohol-treated colony.

The influence of cyproheptadine (CPH) on forced and voluntary ethanol intake was studied in long-term alcohol-treated rats. Wistar rats from a colony (WAC) that have been drinking alcohol as their only liquid fluid for the 25 previous generations and from a genetically related colony (WN) that had never been given alcohol were used. In the first experiment, daily IP doses of 15 mg/kg CPH were found to reduce forced ethanol consumption during CPH treatment in both WAC and WN rats which had been drinking ethanol for six months.

Blood pressure changes after gastric mechanical and electrical stimulation in rats.

The effect of different stimuli applied in several parts of the digestive tract on blood pressure was studied in unconscious Wistar rats. Gastric distension and electrical and pinching stimuli produced a significant increase in both systolic and diastolic arterial blood pressure. When different gastric zones were stimulated by either electrical or pinching stimuli, the highest pressor response was found in the antropyloric zone.

Evolution and characterization of renal immune complexes deposition in mice bearing a syngeneic tumor.

Immune complex deposition along the glomerular basement membrane (GBM) and in the mesangium in C57BL/6 mice bearing a transplantable syngeneic B16 melanoma is described. By immunofluorescence studies, IgG, IgM and C3 were found deposited along the GBM and the mesangium. Electron microscopy showed electron-dense deposits localized in GBM and mesangial matrix.

Metaischemic (post-Goldblatt) hypertensive vascular disease in rats.

Malignant hypertension was induced in rats by aortic ligation above the left renal artery. After 7- and 28-day periods of hypertension, the characteristics of the vascular disease were studied and the kidney below the aortic ligation was removed. The blood pressure and the vascular disease were reexamined at the end of the first and fourth weeks after nephrectomy.

Effects of sodium chloride on early and chronic phases of malignant hypertension in rats.

Malignant hypertension was induced in Sprague-Dawley rats by a complete aortic ligation above the left renal artery. The effects of saline (0.9% NaCl) administration on the development and course of the malignant hypertensive vascular disease were studied and the treated rats were compared to non-saline-treated animals and to sham-operated controls in the early and chronic phases of the disease.

The role of the adrenal cortex in human essential hypertension: keynote address.

The data presented establish that in early or mild essential hypertension there is a state of inappropriate hypermineralocorticoid activity represented by the sum of aldosterone and 18-hydroxy-11-deoxycorticosterone concentrations in the plasma. This disturbance, associated with a "normal" or excessive salt intake, would produce the arteriolar cationic changes in sodium, potassium, calcium, or magnesium leading to hypersensitivity or hyperresponsiveness of the arteriolar actomyosin to normal levels of circulating norepinephrine or angiotensin. The nature of the cationic changes in the arteriolar cells responsible for the increased tonicity of the arteriolar actomyosin, which is the fundamental cause of essential hypertension, remains to be elucidated.

Effects of reserpine on water, cation, and norepinephrine contents of cardiovascular tissues of normotensive dogs.

Six adult male mongrel dogs were treated orally with reserpine (0.03 mg/kg) for 6 weeks, after which time the arterial tissue content of water, cations (sodium, potassium, magnesium, and calcium), and norepinephrine was determined and compared with those of six untreated dogs. Reserpine administration resulted in sustained decreases in the mean arterial blood pressure and heart rate, and the arterial tissues of the treated dogs contained less potassium, calcium, and magnesium, the last-named being affected most, but the sodium remained the same.

The inhibition by substrate analogues of gamma-aminobutyrate aminotransferase from mitochondria of different subcellular fractions of rat brain.

Cytoplasmic-mitochondria-enriched and synaptosome-enriched subcellular fractions were prepared from rat brain to study certain kinetic properties of gamma-aminobutyrate: alpha-oxoglutarate aminotransferase (GABA-T) (EC 2.6.1.

Effects of angiotensin II on steroid metabolism and hepatic blood flow in man.

Metabolic clearance rates (MCR) of aldosterone, cortisol, 11-deoxycorticosterone (DOC), corticosterone, and progesterone were simultaneously measured by constant infusion in eight control subjects before and during angiotensin II infusion in subpressor (3 ng/min per kg) and pressor (22 ng/min per kg) doses. Plasma levels of aldosterone and cortisol, the heat-labile protein-bound fraction of aldosterone, and hepatic blood flow (HBF) (as estimated by the fractional clearance of indocyanine green) were determined concomitantly. Angiotensin II in a subpressor dose produced a significant decrease of the MCR of aldosterone (by 23%), cortisol (by 16%), DOC (by 26%), corticosterone (by 14%) and progesterone (by 33%).

Arterial hypertension and neurofibromatosis: renal artery stenosis and coarctation of abdominal aorta.

A 10-year-old girl had arterial hypertension, generalized neurofibromatosis, coarctation of the abdominal aorta and multiple stenoses at the origin of each renal artery. After resection of the stenotic areas and reimplantation of the renal arteries in the aorta, her arterial pressure decreased substantially. However, hypertension recurred and radiologic follow-up 4 1/2 years later showed distinct progression of the coarctation and renewed stenosis of all renal arteries at their origin.