The effects of alpha-lipoic acid transdermal patch for local subcutaneous fat reduction: A randomized, placebo-controlled, clinical trial in overweight volunteers.

Background: Combating obesity is challenging, as anti-obesity compounds lose effectiveness or cause severe side effects when delivered via conventional routes. Thus, there is a need for new, effective treatment routes that are home-based and safe for long-term use. This double-blind, placebo-controlled clinical trial aimed to investigate the efficacy of a biocellulose transdermal patch containing α-lipoic acid (ALA), an anti-obesity compound, in reducing subcutaneous fat accumulation.

Novel intranasal phage-CaEDTA-ceftazidime/avibactam triple combination therapy demonstrates remarkable efficacy in treating Pseudomonas aeruginosa lung infection.

Given the rise of multidrug-resistant (MDR) Pseudomonas aeruginosa infections, alternative treatments are needed. Anti-pseudomonal phage therapy shows promise, but its clinical application is limited due to the development of resistance and a lack of biofilm penetration. Recently, adjuvants like CaEDTA have shown the ability to enhance the effectiveness of combined antimicrobial agents.

Synthesis and Structure-Property Relationship of -Substituted Porphyrin- and Benzoporphyrin-Thiophene Conjugates toward Electrochemical Reduction of Carbon Dioxide.

A novel series of Zn--AB porphyrins and benzoporphyrins bearing phenyl and thiophene-based -substituents was successfully synthesized and characterized by spectroscopic and electrochemical techniques. Systematic comparison among the compounds in this series, together with the corresponding A analogs previously studied by our group, led to the understanding of the effects of π-conjugated system extension of a porphyrin core through β-fused rings, replacement of the phenyl with the thiophene-based -groups, and introduction of additional thiophene rings on thienyl substituents on photophysical and electrochemical properties. Oxidative electropolymerization through bithiophenyl units of both A and -AB analogs was achieved, resulting in porphyrin- and benzoporphyrin-oligothiophene conjugated polymers, which were characterized by cyclic voltammetry and absorption spectrophotometry.

Targeted Therapy with Cisplatin-Loaded Calcium Citrate Nanoparticles Conjugated with Epidermal Growth Factor for Lung Cancer Treatment.

Lung cancer is the leading cause of cancer-related deaths worldwide with high incidence rates for new cases. Conventional cisplatin (CDDP) therapy has limitations due to severe side effects from nonspecific targeting. To address this challenge, nanomedicine offers targeted therapies.

Ca-EDTA restores the activity of ceftazidime-avibactam or aztreonam against carbapenemase-producing infections.

Developing an effective therapy to overcome carbapenemase-positive (CPKp) is an important therapeutic challenge that must be addressed urgently. Here, we explored a Ca-EDTA combination with aztreonam or ceftazidime-avibactam and against diverse CPKp clinical isolates. The synergy testing of this study demonstrated that novel aztreonam-Ca-EDTA or ceftazidime-avibactam-Ca-EDTA combination was significantly effective in eliminating planktonic and mature biofilms , as well as eradicating CPKp infections .

"Assessing exposure of printing factory workers in thailand to selected heavy metals using urine and hair as non-invasive matrices".

Background: There are few thorough studies on the extent and inter-element relationships of heavy metal contamination in printing factory workers, especially in developing countries. The objective of this study was to determine the levels of eight heavy metals, including arsenic (As), cadmium (Cd), chromium (Cr), nickel (Ni), cobalt (Co), lead (Pb), mercury (Hg), and manganese (Mn), in urine and scalp hair of printing industry workers, and assess inter-element correlations.

Methods: We examined a total of 85 urine samples and 85 scalp hair samples (3 cm hair segments taken from near the scalp) in 85 printing workers from a printing house in Bangkok, Thailand.

Development of Eugenol-Embedded Calcium Citrate Nanoparticles as a Local Anesthetic Agent.

Eugenol is a major phenolic component derived from clove oil with potential medical applications. Of particular interest, it has been used as a therapeutic agent in topical applications because of its analgesic and local anesthetic properties. However, topical formulations of eugenol produce skin irritation, which limits its clinical applications.

Novel colistin-EDTA combination for successful eradication of colistin-resistant Klebsiella pneumoniae catheter-related biofilm infections.

Development of an effective therapy to overcome colistin resistance in Klebsiella pneumoniae, a common pathogen causing catheter-related biofilm infections in vascular catheters, has become a serious therapeutic challenge that must be addressed urgently. Although colistin and EDTA have successful roles for eradicating biofilms, no in vitro and in vivo studies have investigated their efficacy in catheter-related biofilm infections of colistin-resistant K. pneumoniae.

Biguanide-Based Synthesis of 1,3,5-Triazine Derivatives with Anticancer Activity and 1,3,5-Triazine Incorporated Calcium Citrate Nanoparticles.

Twelve derivatives of biguanide-derived 1,3,5-triazines, a promising class of anticancer agent, were synthesised and evaluated for their anticancer activity against two colorectal cancer cell lines-HCT116 and SW620. and which are the derivatives containing -hydroxyphenyl substituents exhibited the highest activity with IC against both cell lines in the range of 20-27 µM, which is comparable to the IC of cisplatin reference. Moreover, the potential use of the calcium citrate nanoparticles (CaCit NPs) as a platform for drug delivery system was studied on a selected 1,3,5-triazine derivative .

Effect of Gold Nanoparticles on the TLR2-Mediated Inflammatory Responses Induced by in TLR2-Overexpressed HEK293 Cells.

infection can cause potential hazards to human health by stimulating inflammation, which is mediated mainly through the Toll-like receptor 2 (TLR2) pathway. Gold nanoparticles (AuNPs) are promising for medical applications, as they display both bioinert and noncytotoxic characteristics. AuNPs have been shown to have the ability to modify immune responses.

Feeding Cells with a Novel "Trojan" Carrier: Citrate Nanoparticles.

In this work, the preparation of novel calcium citrate (CaCit) nanoparticles (NPs) has been disclosed and the use of these NPs as "Trojan" carriers has been demonstrated. The concentration ratio between calcium ions and citrate ions was optimized, yielding spherical NPs with size in the range of 100-200 nm. Additionally, a fluorescent dye, fluorescein isothiocyanate (FITC), was successfully encapsulated by the coprecipitation method.

Sustaining Antibiotic Release from a Poly(methyl methacrylate) Bone-Spacer.

One of the challenges in using a bone-spacer to cure infection is the fabrication of a material that can continuously release required antibiotics at effective concentrations for at least 4-6 weeks. Poly(methyl methacrylate) (PMMA) impregnated with antibiotics is one of the popularly used bone-spacer materials. Currently, improved sustained release of hydrophobic and hydrophilic antibiotics is needed for this material.

Molecular and Morphological Evidence of Hepatotoxicity after Silver Nanoparticle Exposure: A Systematic Review, , and Ultrastructure Investigation.

Silver nanoparticles (AgNPs) have been widely used in a variety of applications in innovative development; consequently, people are more exposed to this particle. Growing concern about toxicity from AgNP exposure has attracted greater attention, while questions about nanosilver-responsive genes and consequences for human health remain unanswered. By considering early detection and prevention of nanotoxicology at the genetic level, this study aimed to identify 1) changes in gene expression levels that could be potential indicators for AgNP toxicity and 2) morphological phenotypes correlating to toxicity of HepG2 cells.

N-acetylcysteine reverses the decrease of DNA methylation status caused by engineered gold, silicon, and chitosan nanoparticles.

Engineered nanoparticles (ENPs) are one of the most widely used types of nanomaterials. Recently, ENPs have been shown to cause cellular damage by inducing ROS (reactive oxygen species) both directly and indirectly, leading to the changes in DNA methylation levels, which is an important epigenetic mechanism. In this study, we investigated the effect of ENP-induced ROS on DNA methylation.

Glutaraldehyde erythropoietin protects kidney in ischaemia/reperfusion injury without increasing red blood cell production.

Background And Purpose: Recombinant human erythropoietin (rHuEPO) is currently the mainstay of renal anaemia treatment. Recently, rHuEPO has been shown to provide pleiotrophic tissue protection in various pathological conditions. However, the benefits of rHuEPO beyond anaemia treatment are limited because it increases red blood cell mass.

Enhanced agglutination reaction of ABO subgroups by gold nanoparticle solution: implication for identification of ABO subgroups.

Although the ABO blood group is the most significant in blood group system in human, other subgroups system is also important to be concerned in blood banking laboratory. ABO subgroups have weak antigen potency on red blood cell. In some cases, they could not been detected by cell grouping and serum grouping methods.

Gold nanoparticles and a microscopic view of platelets: a preliminary observation.

There is limited information on the use of nanoparticles in medicine, and in particular, the effect of gold nanoparticles on human platelets. We carried out an experimental study at Chulalongkorn University, Bangkok, Thailand to demonstrate the direct effect of gold nanoparticles on platelets. A solution of gold nanoparticles was mixed with a blood sample and analysed.

Identification of gold nanoparticle in lymphocytes: a confirmation of direct intracellular penetration effect.

Objective: Nanoparticles differ from the same material at larger scale in chemical and physical properties. The effect of nanoparticle on the blood cell still needs scientific verification.

Methods: The direct effect of gold nanoparticles on lymphocyte was direct assessed by in vitro assay.

Gold nanoparticle as an alternative tool for a urine pregnancy test.

Objective: The urine pregnancy test is an easily available diagnostic test in the present day and is routinely performed. The test is based on an immunochromatography technique. Here, we used an advanced nanomedicine technique for modification of the urine pregnancy test.

Electronic absorption spectroscopy probed side-chain movement in chromic transitions of polydiacetylene vesicles.

Thermochromism, solvatochromism, and alkalinochromism of a poly-10,12-pentacosadiynoic acid (poly(PCDA)) vesicle solution are studied by electronic absorption spectroscopy. The spectroscopic profiles reveal different sequences of side-chain movement during the chromic transitions. The gradual hypsochromic shift and reversibility of the purple solution at low temperature in the thermochromic transition indicates that the transition starts with reversible conformational alteration of methylene side chains leading to metastable purple vesicles.

Effect of gold nanoparticle on renal cell: an implication for exposure risk.

Objective: The objective of this study was to evaluate the effect of gold nanoparticles on renal cell.

Intervention: This study was performed as an experimental study. A mixture of gold nanoparticle solution and renal cell sediment was prepared and further analyzed.

Effect of gold nanoparticle on the microscopic morphology of white blood cell.

Background: In medicine, there is limited knowledge on the toxicity of nanoparticles. In medicine, there has been limited knowledge on the effect of nanoparticles on the white blood cell.

Objective: To evaluate the effect of gold nanoparticle on the microscopic morphology of white blood cell.

Gold nanoparticle as an alternative tool for urine microalbumin test: the first world report.

Unlabelled: Urine microalbuminuria is a common indicator for occult preclinical nephropathy. At present, urine microalbumin test is routinely performed by clinical chemistry test. Here, the authors used the advanced nanomedicine technique for modification of the urine microalbumin test.

Effect of gold nanoparticles on spermatozoa: the first world report.

Objective: To evaluate the spermatotoxicity of gold nanoparticles.

Design: Experimental study.

Setting: A university in Thailand.

Increasing the agglutination reaction in slide test for weak B blood group by gold nanoparticle solution: the first world report.

Objective: Blood group analysis is a basic immunohematological test. It should be noted that A, B, O and AB have various subgroups. Here, the authors used the advanced nanomedicine technique for modification of the basic blood group test for detection of weak B subgroup.