Enzymatic properties and physiological roles of cytosolic 5'-nucleotidase II.

Cytosolic 5'-nucleotidase II (cN-II) is an intracellular 5'-nucleotidase characterized by substrate specificity. It preferentially hydrolyzes 6-hydroxypurine nucleotides such as IMP and GMP over AMP or UMP. cN-II is allosterically activated by ATP and inhibited by inorganic phosphate.

IMP-GMP 5'-nucleotidase in reptiles: occurrence and tissue distribution in a crocodile and three species of lizards.

IMP-hydrolyzing activity (which is reactive with goose anti-pig lung IMP-GMP 5'-nucleotidase (c-N-II: EC.3.1.

IMP-GMP 5'-nucleotidase in reptiles: occurrence in a turtle, a tortoise and three species of snakes.

IMP-hydrolyzing activity, which is reactive with goose anti-pig lung IMP-GMP 5'-nucleotidase (c-N-II: EC.3.1.

The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5'-nucleotidase.

Background: The purine salvage enzyme inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase catalyzes degradation of IMP to inosine. Although this enzymatic activity has been purified and characterized in Saccharomyces cerevisiae, the gene encoding IMP 5'-nucleotidase had not been identified.

Results: Mass spectrometry analysis of several peptides of this enzyme purified from yeast allowed identification of the corresponding gene as YOR155c, an open reading frame of unknown function, renamed ISN1.

Occurrence of IMP-GMP 5'-nucleotidase in three fish species: a comparative study on Trachurus japonicus, Oncorhynchus masou masou and Triakis scyllium.

IMP-hydrolyzing activity, which is reactive with goose anti-pig lung IMP-GMP 5'-nucleotidase (EC.3.1.