Solid-state fermentation-based enzyme-assisted extraction of eicosapentaenoic acid-rich oil from Nannochloropsis sp.

Enzymatic treatment of microalgal biomass is a promising approach for extraction of microalgal lipid, but high cost of commercially sourcing enzyme is a major drawback in industrial implementation. Present study involves extraction of eicosapentaenoic acid-rich oil from Nannochloropsis sp. biomass using low cost cellulolytic enzymes produced from Trichoderma reesei in a solid-state fermentation bioreactor.

Sustainable production of eicosapentaenoic acid-rich oil from microalgae: Towards an algal biorefinery.

Utilization of sustainable natural resources such as microalgae has been considered for the production of biofuels, aquaculture feed, high-value bioactives such as omega-3 fatty acids, carotenoids, etc. Eicosapentaenoic acid (EPA) is an omega-3 fatty acid present in fish oil, which is of physiological importance to both humans and fish. Marine microalgae are sustainable sources of lipid rich in EPA, and different species have been explored for the production of EPA as a single product.