Harnessing the Potential of Biological Recognition Elements for Water Pollution Monitoring.

Environmental monitoring of pollutants is an imperative first step to remove the genotoxic, embryotoxic, and carcinogenic toxins. Various biological sensing elements such as proteins, aptamers, whole cells, etc., have been used to track down major pollutants, including heavy metals, aromatic pollutants, pathogenic microorganisms, and pesticides in both environmental samples and drinking water, demonstrating their potential in a true sense.

Tunable Multiplexed Whole-Cell Biosensors as Environmental Diagnostics for ppb-Level Detection of Aromatic Pollutants.

Aromatics such as phenols, benzene, and toluene are carcinogenic xenobiotics which are known to pollute water resources. By employing synthetic biology approaches combined with a structure-guided design, we created a tunable array of whole-cell biosensors (WCBs). The MopR genetic system that has the natural ability to sense and degrade phenol was adapted to detect phenol down to ∼1 ppb, making this sensor capable of directly detecting phenol in permissible limits in drinking water.