Biological control of gastrointestinal nematodiasis in ruminants is an alternative to reduce the number of infective larvae. The fungal isolates predatory activity preservation is a basic requirement for the success of this control type. The aim of this work is to evaluate the predatory capacity of the fungus Arthrobotrys robusta (isolate I-31), preserved on silica gel on infective larvae of Haemonchus contortus under laboratory conditions on 2 % water agar (2 % WA).
View Article and Find Full Text PDFThe aim of this study was to evaluate the predatory activity of the fungus Duddingtonia flagrans (AC001) on infective larvae of Ancylostoma ceylanicum after gastrointestinal transit in hamsters. Twenty animals were used in the experiment, divided into two groups: a treated group (10 animals) and a control group (10 animals). In the group treated with D.
View Article and Find Full Text PDFIntroduction: Strongyloides venezuelensis has been used as a model for studying human strongyloidosis.
Methods: This study aimed to compare the ability of predatory nematophagous fungi Duddingtonia flagrans (AC001), Arthrobotrys robusta (I-31) and Monacrosporium sinense (SF53) and on infective larvae (L3) of Strongyloides venezuelensis in laboratory conditions on 2% water-agar medium.
Results: At the end of the experiment, the percentage reductions of Strongyloides venezuelensi L3 were: 93% (AC001), 77.
Introduction: Ancylostoma sp is a potentially zoonotic geohelminth.
Methods: This study aimed to evaluate in vitro the action of crude enzyme extract of Pochonia chlamydosporia (VC4) on eggs of Ancylostoma sp in 2% water-agar and in fecal cultures.
Results: The percentage reduction in Ancylostoma sp egg eclosion was 76.
Viability and in vitro and in vivo activities of freeze-dried conidia of the predatory fungus Arthrobotrys robusta (I-31) were evaluated against infective larvae (L(3)) of Ancylostoma spp. in dogs. A.
View Article and Find Full Text PDFThe ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water-agar with seven fungal isolates (Isol.
View Article and Find Full Text PDFRev Soc Bras Med Trop
April 2011
Introduction: Strongyloides stercoralis is a nematode that infects much of the population worldwide.
Methods: This study aimed to compare the ability of predatory nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Arthrobotrys robusta (I-31) on infective larvae (L₃) of Strongyloides stercoralis in laboratory conditions on 2% water-agar.
Results: At the end of the experiment, the percentage reductions in Strongyloides stercoralis L3 were 83.
Vet Parasitol
January 2011
Horses are hosts to a wide variety of helminthes; the most important are the cyathostomin, or small strongyles. The viability of a fungal formulation (pellets) using the nematode-trapping fungus Monacrosporium thaumasium was assessed in biological control of horse cyathostomin. Two groups (fungus-treated and control) consisted of six mares in each group, crossbred (ages of 2.
View Article and Find Full Text PDFThe aims of this study were to test the action of the fungal extract of Pochonia chlamydosporia (VC4) on the hatching of cyathostomin eggs plated in Petri dishes containing 2% water-agar (2% WA) and its enzymatic activity in fecal cultures, in two experimental assays (A and B). The fungus P. chlamydosporia (VC4) was cultured in Erlenmeyer flasks (250ml) containing 50ml of liquid minimal medium supplemented with 0.
View Article and Find Full Text PDFThe potential role of companion animals as reservoirs for zoonotic diseases has been recognised as a significant public health problem worldwide. Ancylostoma ceylanicum is the only ancylostomatidae species known for infecting human beings. This article aimed to compare the predatory capacity of predatory fungi isolates Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34), Monacrosporium sinense (SF53) and Arthrobotrys robusta (I31) on A.
View Article and Find Full Text PDFThe predatory capacity of one isolate of nematode-trapping fungus Duddingtonia flagrans (AC001) on infective larvae of cyathostomes was evaluated in laboratorial conditions in medium water-agar 2% (WA 2%). There was significant reduction (p<0.01) of 93.
View Article and Find Full Text PDFThree isolates of predator fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34), and Arthrobotrys robusta (I-31) were assessed in in vitro test regarding the capacity of prey infective larvae (L(3)) Strongyloides westeri. Compared to control, without fungus, there was a significant decrease (P < 0.01) of 80.
View Article and Find Full Text PDFThis work was performed to determine the predatory capacity in vitro of the nematophagous fungus Duddingtonia flagrans (isolate AC001) on cyathostomin infective larvae of horse (L(3)). The experimental assay was carried out on plates with 2% water-agar (2% WA). In the treated group, each plate contained 1.
View Article and Find Full Text PDFThe predatory capacity of the nematophagous fungus Pochonia chlamydosporia (isolate VC4) embedded in sodium alginate pellets after passage through the gastrointestinal tract of horses was assessed in vitro against Oxyuris equi eggs. Twelve previously dewormed crossbred mares, average weight of 362.5kg (+/-21) were used in the experiment.
View Article and Find Full Text PDFFormulations in matrix of sodium alginate (pellets) of the nematode predatory fungi Duddingtonia flagrans and Monacrosporium thaumasium were evaluated in the biological control of sheep gastrointestinal nematodiasis. Three groups (1, 2, and 3), each one with eight sheep of the Santa Inês breed, at the ages of 15-48 months, were placed in paddocks of Brachiaria decumbens for 5 months. In group 1, each animal received 1 g/10 kg of live weight (l.
View Article and Find Full Text PDFNematophagous fungi are potential biological control agents of helminths. The in vitro ovicidal effect of four isolates of the nematophagous fungi Pochonia chlamydosporia (VC1 and VC4), Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) was evaluated on egg capsules of Dipylidium caninum, a cestode parasite of dogs, cats and humans. One thousand egg capsules of D.
View Article and Find Full Text PDFToxocara (Neoascaris) vitulorum is a gastrointestinal nematode parasite of young ruminants, responsible for high mortality rates in parasitized cattle and buffalo calves. The objective of this work was to compare the predatory capacity under laboratory conditions of four fungal isolates of the nematophagous fungus Pochonia chlamydosporia (VC1, VC4, VC5 and VC12) on T. vitulorum eggs in 2% water-agar (2% WA).
View Article and Find Full Text PDFVet Parasitol
August 2009
The viability of a fungal formulation using the nematode-trapping fungus Duddingtonia flagrans was assessed for the biological control of horse cyathostomin. Two groups (fungus-treated and control without fungus treatment), consisting of eight crossbred mares (3-18 years of age) were fed on Cynodon sp. pasture naturally infected with equine cyathostome larvae.
View Article and Find Full Text PDFWith the aim of demonstrating the effectiveness of the fungus Paecilomyces lilacinus on Taenia saginata eggs under laboratory conditions, a trial was set up in Petri dishes with water-agar 2%. There was ovicidal activity (p < 0.05) in relation to the control group on the tenth day of interaction and an internal colonization rate of 25.
View Article and Find Full Text PDFThe ovicidal activity of the nematophagous fungi Pochonia chlamydosporia (isolates VC1 and VC4), Duddingtonia flagrans (isolate AC001) and Monacrosporium thaumasium (isolate NF34) on Taenia saginata eggs was evaluated under laboratory conditions. T. saginata eggs were plated on 2% water-agar with fungal isolates and controls without fungus and examined after 5, 10 and 15 days.
View Article and Find Full Text PDFThe present study was performed in order to evaluate the action of anthelmintics compounds on gastrointestinal parasite nematodes of 27 Alpine and Saanen adult goats. The animals were divided into three groups. The animals of groups 1 and 2 had been dealt with two different associations of antihelminthics in day zero.
View Article and Find Full Text PDFThe in vitro action of the nematophagous fungi Duddingtonia flagrans, Monacrosporium thaumasium and Verticillium chlamydosporium on eggs of Ascaris lumbricoides was observed. After 7, 10 and 14 days of interaction, the fungus showing most promise for use in biologically control over Ascaris lumbricoides was Verticillium chlamydosporium (26-30%). The other fungi did not present satisfactory results.
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