Diving into the metabolic interactions of titanium dioxide nanoparticles in "Sparus aurata" and "Ruditapes philippinarum".

The biological response to nanomaterials exposure depends on their properties, route of exposure, or model organism. Titanium dioxide nanoparticles (TiO NPs) are among the most used nanomaterials; however, concerns related to oxidative stress and metabolic effects resulting from their ingestion are rising. Therefore, in the present work, we addressed the metabolic effects of citrate-coated 45 nm TiO NPs combining bioaccumulation, tissue ultrastructure, and proteomics approaches on gilthead seabream, Sparus aurata and Japanese carpet shell, Ruditapes philippinarum.

Bioaccumulation, biodistribution, and transformation of polyvinylpyrrolidone-coated silver nanoparticles in edible seaweeds.

Seaweeds are recognised as a potential eco-friendly food source. However, some species have shown the capacity to bioaccumulate many substances of diverse nature, such as inorganic nanoparticles (NPs), which may have potentially harmful effects on them. Among these NPs, silver nanoparticles (AgNPs) have been used to enhance the antifungal and antibacterial properties of the final consumer products, such as textiles and food packages.

Platelet-rich fibrin (PRF) modified nano-hydroxyapatite/chitosan/gelatin/alginate scaffolds increase adhesion and viability of human dental pulp stem cells (DPSC) and osteoblasts derived from DPSC.

Bone tissue regeneration strategies have incorporated the use of natural polymers, such as hydroxyapatite (nHA), chitosan (CH), gelatin (GEL), or alginate (ALG). Additionally, platelet concentrates, such as platelet-rich fibrin (PRF) have been suggested to improve scaffold biocompatibility. This study aimed to develop scaffolds composed of nHA, GEL, and CH, with or without ALG and lyophilized PRF, to evaluate the scaffold's properties, growth factor release, and dental pulp stem cells (DPSC), and osteoblast (OB) derived from DPSC viability.

Submicron- and nanoplastic detection at low micro- to nanogram concentrations using gold nanostar-based surface-enhanced Raman scattering (SERS) substrates.

The presence of submicron- (1 μm-100 nm) and nanoplastic (<100 nm) particles within various sample matrices, ranging from marine environments to foods and beverages, has become a topic of increasing interest in recent years. Despite this interest, very few analytical techniques are known that allow for the detection of these small plastic particles in the low concentration ranges that they are anticipated to be present at. Research focused on optimizing surface-enhanced Raman scattering (SERS) to enhance signal obtained in Raman spectroscopy has been shown to have great potential for the detection of plastic particles below conventional resolution limits.

New versus naturally aged greenhouse cover films: Degradation and micro-nanoplastics characterization under sunlight exposure.

The understanding of microplastic degradation and its effects remains limited due to the absence of accurate analytical techniques for detecting and quantifying micro- and nanoplastics. In this study, we investigated the release of nanoplastics and small microplastics in water from low-density polyethylene (LDPE) greenhouse cover films under simulated sunlight exposure for six months. Our analysis included both new and naturally aged (used) cover films, enabling us to evaluate the impact of natural aging.

Evaluation of the Antimicrobial Activity of Chitosan Nanoparticles against .

Chitosan is obtained from the deacetylation of chitin, and it is known to possess antimicrobial activity. It has attracted attention as it may be used for treating infections caused by different types of microorganisms due to its broad spectrum. Its application in the form of micro- or nanoparticles (CM/CN) has expanded its usage, as in this form, it retains its activity, and remain stable in aqueous solutions.

Developing an improved optical biosensing system based on gold nanoparticles acting as interferometric enhancers in Lactoferrin detection.

We report for the first time the whole development of a biosensing system based on the Interferometric Optical Detection Method (IODM) enriched with gold nanoparticles (AuNPs), acting as interferometric enhancers for improving the performance of immunoassays. For this purpose, the Lactoferrin sandwich immunoassay model was employed. We describe in detail the entire value chain from the AuNPs production, its functionalization, and characterization with anti-Lactoferrin (anti-LF), the biosensing response of these conjugates as well as their corresponding calculation of the kinetic constants, performance comparison of the readout interferometric signals Scanning Electron Microscopy (SEM) and the percentage of the sensing surface covered.

Acute Aquatic Toxicity to Zebrafish and Bioaccumulation in Marine Mussels of Antimony Tin Oxide Nanoparticles.

Antimony tin oxide (SbO/SnO) is effective in the absorption of infrared radiation for applications, such as skylights. As a nanoparticle (NP), it can be incorporated into films or sheets providing infrared radiation attenuation while allowing for a transparent final product. The acute toxicity exerted by commercial SbO/SnO (ATO) NPs was studied in adults and embryos of zebrafish ().

Bioaccumulation of titanium dioxide nanoparticles in green (Ulva sp.) and red (Palmaria palmata) seaweed.

A bioaccumulation study in red (Palmaria palmata) and green (Ulva sp.) seaweed has been carried out after exposure to different concentrations of citrate-coated titanium dioxide nanoparticles (5 and 25 nm) for 28 days. The concentration of total titanium and the number and size of accumulated nanoparticles in the seaweeds has been determined throughout the study by inductively coupled plasma mass spectrometry (ICP-MS) and single particle-ICP-MS (SP-ICP-MS), respectively.

Getting fat and stressed: Effects of dietary intake of titanium dioxide nanoparticles in the liver of turbot Scophthalmus maximus.

The extensive use of nanomaterials, including titanium dioxide nanoparticles (TiO NPs), raises concerns about their persistence in ecosystems. Protecting aquatic ecosystems and ensuring healthy and safe aquaculture products requires the assessment of the potential impacts of NPs on organisms. Here, we study the effects of a sublethal concentration of citrate-coated TiO NPs of two different primary sizes over time in flatfish turbot, Scophthalmus maximus (Linnaeus, 1758).

Cobalt Impregnation on Titania Photocatalysts Enhances Vis Phenol Photodegradation.

One of the main challenges of photocatalysis is to find a stable and effective photocatalyst, that is active and effective under sunlight. Here, we discuss the photocatalytic degradation of phenol as a model pollutant in aqueous solution using NUV-Vis (>366 nm) and UV (254 nm) in the presence of TiO-P25 impregnated with different concentrations of Co (0.1%, 0.

Tailoring/Tuning Properties of Polyester Urea-Urethanes through Hybridization with Titania Obtained Using the Sol-Gel Process.

Hybrid materials have been studied because in these materials the properties of organic components, such as elasticity and biodegradability, could be combined with the properties of inorganic components, such as good biological response, thereby transforming them into a single material with improved properties. In this work, Class I hybrid materials based on polyester-urea-urethanes and titania were obtained using the modified sol-gel method. This was corroborated using the FT-IR and Raman techniques which highlighted the formation of hydrogen bonds and the presence of Ti-OH groups in the hybrid materials.

Single-cell ICP-MS for studying the association of inorganic nanoparticles with cell lines derived from aquaculture species.

The current research deals with the use of single-cell inductively coupled plasma-mass spectrometry (scICP-MS) for the assessment of titanium dioxide nanoparticle (TiO NP) and silver nanoparticle (Ag NP) associations in cell lines derived from aquaculture species (sea bass, sea bream, and clams). The optimization studies have considered the avoidance of high dissolved background, multi-cell peak coincidence, and possible spectral interferences. Optimum operating conditions were found when using a dwell time of 50 μs for silver and 100 μs for titanium.

Low-intensity continuous ultrasound to inhibit cancer cell migration.

In recent years, it has been verified that collective cell migration is a fundamental step in tumor spreading and metastatic processes. In this paper, we demonstrate for the first time how low-intensity ultrasound produces long-term inhibition of collective migration of epithelial cancer cells in wound healing processes. In particular, we show how pancreatic tumor cells, PANC-1, grown as monolayers respond to these waves at frequencies close to 1 MHz and low intensities (<100 mW cm) for 48-72 h of culture after some minutes of a single ultrasound irradiation.

Innovative Antibacterial, Photocatalytic, Titanium Dioxide Microstructured Surfaces Based on Bacterial Adhesion Enhancement.

Bacterial colonization and biofilm formation are found on nearly all wet surfaces, representing a serious problem for both human healthcare and industrial applications, where traditional treatments may not be effective. Herein, we describe a synergistic approach for improving the performance of antibacterial surfaces based on microstructured surfaces that embed titanium dioxide nanoparticles (TiO NPs). The surfaces were designed to enhance bacteria entrapment, facilitating their subsequent eradication by a combination of UVC disinfection and TiO NPs photocatalysis.

Generation of Controlled Micrometric Fibers inside Printed Scaffolds Using Standard FDM 3D Printers.

New additive manufacturing techniques, such as melting electro-writing (MEW) or near-field electrospinning (NFES), are now used to include microfibers inside 3D printed scaffolds as FDM printers present a limited resolution in the XY axis, not making it easy to go under 100 µm without dealing with nozzle troubles. This work studies the possibility of creating reproducible microscopic internal fibers inside scaffolds printed by standard 3D printing. For this purpose, novel algorithms generating deposition routines (G-code) based on primitive geometrical figures were created by python scripts, modifying basic deposition conditions such as temperature, speed, or material flow.

Threefold reactivity of a COF-embedded rhenium catalyst: reductive etherification, oxidative esterification or transfer hydrogenation.

The reactivity of the novel Re(I) catalyst [Re(Anth-py)(CO)Br] is modulated by its interactions with the covalent organic framework (COF) TFB-BD. The complex catalyzes either reductive etherification, oxidative esterification, or transfer hydrogenation depending on its local environment (embedded in TFB-BD, in homogeneous solution or co-incubated with TFB-BD, respectively). The results highlight that COFs can drastically modulate the reactivity of homogeneous catalysts.

Proteomics reveals multiple effects of titanium dioxide and silver nanoparticles in the metabolism of turbot, Scophthalmus maximus.

Titanium dioxide (TiO) and silver (Ag) NPs are among the most used engineered inorganic nanoparticles (NPs); however, their potential effects to marine demersal fish species, are not fully understood. Therefore, this study aimed to assess the proteomic alterations induced by sub-lethal concentrations citrate-coated 25 nm ("P25") TiO or polyvinylpyrrolidone (PVP) coated 15 nm Ag NPs to turbot, Scophthalmus maximus. Juvenile fish were exposed to the NPs through daily feeding for 14 days.

Fundamentals of Biosensors and Detection Methods.

Biosensors have a great impact on our society to enhance the life quality, playing an important role in the development of Point-of-Care (POC) technologies for rapid diagnostics, and monitoring of disease progression. COVID-19 rapid antigen tests, home pregnancy tests, and glucose monitoring sensors represent three examples of successful biosensor POC devices. Biosensors have extensively been used in applications related to the control of diseases, food quality and safety, and environment quality.