Publications by authors named "Rodrigo S C Brant"
Characterization of a novel acidic phospholipase A isolated from the venom of Bothrops mattogrossensis: From purification to structural modeling.
Int J Biol Macromol
December 2024
Phospholipases A (PLAs) are highly prevalent in Bothrops snake venom and play a crucial role in inflammatory responses and immune cell activation during envenomation. Despite their significance, the specific role of PLAs from Bothrops mattogrossensis venom (BmV) in inflammation is not fully understood. This study sought to isolate and characterize a novel acidic PLA from BmV, designated BmPLA-A, and to evaluate its effects on human umbilical vein endothelial cells (HUVECs), with a specific focus on cytotoxicity, adhesion, and detachment.
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- This data descriptor introduces a specialized dataset focused on identifying pathogens like Staphylococcus aureus and Pseudomonas aeruginosa from whole blood samples using advanced mass spectrometry techniques.
- The dataset employs a unique three-tier system, which includes spectral libraries for identifying biomarkers, MS data for optimizing biomarker panels, and PRM data from sepsis patients, achieving 83.3% sensitivity in under seven hours.
- It aims to serve as a vital resource for developing bioinformatic tools and proposing biomarker panels, ultimately enhancing pathogen detection and supporting studies on antimicrobial resistance and epidemiology.
A hydrolyzed N-propionylthiosuccinimide linker is cleaved by metastable fragmentation, increasing reliability of conjugation site identification in conjugate vaccines.
Rapid Commun Mass Spectrom
September 2024
Article Synopsis
- Conjugate vaccines have special places (conjugation sites) that help them work better, and finding these sites is very important for their quality.
- Scientists used advanced techniques like mass spectrometry (MS) to study certain peptides and confirm how they break apart to reveal these conjugation sites.
- The study showed that a specific linker in these peptides can be easily broken down, allowing better identification of conjugation sites, which could lead to stronger vaccines in the future.
The genetic manipulation of Trypanosoma cruzi continues to be a challenge, mainly due to the lack of available and efficient molecular tools. The CRE-lox recombination system is a site-specific recombinase technology, widely used method of achieving conditional targeted deletions, inversions, insertions, gene activation, translocation, and other modifications in chromosomal or episomal DNA. In the present study, the CRE-lox system was adapted to expand the current genetic toolbox for this hard-to-manipulate parasite.
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