Toll like receptor 4 mediates the inhibitory effect of SARS-CoV-2 spike protein on proximal tubule albumin endocytosis.

Tubular proteinuria is a common feature in COVID-19 patients, even in the absence of established acute kidney injury. SARS-CoV-2 spike protein (S protein) was shown to inhibit megalin-mediated albumin endocytosis in proximal tubule epithelial cells (PTECs). Angiotensin-converting enzyme type 2 (ACE2) was not directly involved.

O-Linked GlcNAcylation mediates the inhibition of proximal tubule (Na+K)ATPase activity in the early stage of diabetes mellitus.

Background: Diabetic kidney disease (DKD) is a severe complication of diabetes mellitus (DM). It has been proposed that modifications in the function of proximal tubule epithelial cells (PTECs) precede glomerular damage during the onset of DKD. This study aimed to identify modifications in renal sodium handling in the early stage of DM and its molecular mechanism.

Rapamycin treatment induces tubular proteinuria: role of megalin-mediated protein reabsorption.

Rapamycin is an immunosuppressor that acts by inhibiting the serine/threonine kinase mechanistic target of rapamycin complex 1. Therapeutic use of rapamycin is limited by its adverse effects. Proteinuria is an important marker of kidney damage and a risk factor for kidney diseases progression and has been reported in patients and animal models treated with rapamycin.

Multi-Parameter Optical Monitoring Solution Applied to Underground Medium-Voltage Electric Power Distribution Networks.

This work presents a multi-parameter optical fiber monitoring solution applied to an underground power distribution network. The monitoring system demonstrated herein uses Fiber Bragg Grating (FBG) sensors to measure multiple parameters, such as the distributed temperature of the power cable, external temperature and current of the transformers, liquid level, and intrusion in the underground manholes. To monitor partial discharges of cable connections, we used sensors that detect radio frequency signals.

Gold nanoparticles reduce tubule-interstitial injury and proteinuria in a murine model of subclinical acute kidney injury.

Subclinical acute kidney injury (subAKI) is characterized by tubule-interstitial injury without significant changes in glomerular function. SubAKI is associated with the pathogenesis and progression of acute and chronic kidney diseases. Currently, therapeutic strategies to treat subAKI are limited.

Kidney functions adaptations of professional soccer players in response to an entire game season.

This study investigated the renal function of soccer players after an entire game-season. Thirty-five athletes recruited to play for the Macae Futebol Clube were invited for this study, of which 18 athletes completed the entire game season. Blood and 24-hour urine were collected at the beginning (Pre-Season) and the end of the game season (Post-Season).

Subclinical Acute Kidney Injury in COVID-19: Possible Mechanisms and Future Perspectives.

Since the outbreak of COVID-19 disease, a bidirectional interaction between kidney disease and the progression of COVID-19 has been demonstrated. Kidney disease is an independent risk factor for mortality of patients with COVID-19 as well as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection leading to the development of acute kidney injury (AKI) and chronic kidney disease (CKD) in patients with COVID-19. However, the detection of kidney damage in patients with COVID-19 may not occur until an advanced stage based on the current clinical blood and urinary examinations.

SARS-CoV-2 spike protein inhibits megalin-mediated albumin endocytosis in proximal tubule epithelial cells.

Patients with COVID-19 have high prevalence of albuminuria which is used as a marker of progression of renal disease and is associated with severe COVID-19. We hypothesized that SARS-CoV-2 spike protein (S protein) could modulate albumin handling in proximal tubule epithelial cells (PTECs) and, consequently contribute to the albuminuria observed in patients with COVID-19. In this context, the possible effect of S protein on albumin endocytosis in PTECs was investigated.

Agreement Between Alberta Infant Motor Scale Assessment and Maternal Perception of Motor Development in Full-Term Infants.

Objectives: To verify the agreement between the Alberta Infant Motor Scale assessment and maternal perception of the motor development in full-term infants.

Methods: This is a cross-sectional study involving 161 infants and mothers. Children were assessed with the Alberta Infant Motor Scale (AIMS) for motor developmental classification.

Techniques and Materials for Optical Fiber Sensors Sealing in Dynamic Environments with High Pressure and High Temperature.

We detail a study of the techniques and sealing materials for optical fiber sensors used in dynamic environments with high pressure (>300 bar) and high temperature (>300 °C). The sealing techniques and materials are the key for the robustness of sensors in harsh dynamic environments, such as large combustion engines. The sealing materials and techniques studied in this work are high-temperature epoxies, metallic polymer, metallic solders, glass solder, cement, brazing and electroless nickel plating.

[Evidence of the validity of a Brazilian version of the Fear of COVID-19 Scale].

This study sought to analyze the evidence of the validity of a Brazilian version of the Fear of COVID-19 Scale (FCV-19S), based on indicators relating to: (1) the internal structure; (2) the internal consistency; (3) the relation with external variables; and (4) the content. The instrument was culturally adapted, focusing on semantic and specifically Brazilian linguistic aspects. The Brazilian version of the FCV-19S was then applied, in a virtual environment, to 211 participants (72.

Grieves and struggles of family caregivers providing care for bedridden elderly patients affected by chronic degenerative diseases.

Objectives: To understand grieves and struggles of family caregivers providing care for bedridden elderly patients affected by chronic degenerative diseases.

Methods: This cross-sectional study was developed following the guidelines of the clinical-qualitative method. The sample was composed of 10 female family caregivers of bedridden elderly patients affected by chronic degenerative diseases in a city in the interior of Minas Gerais, Brazil.

Power-over-Fiber LPIT for Voltage and Current Measurements in the Medium Voltage Distribution Networks.

In this work, we present the design, laboratory tests, and the field trial results of a power-over-fiber (PoF) low power instrument transformer (LPIT) for voltage and current measurements in the medium voltage distribution networks. The new proposed design of this power-over-fiber LPIT aims to overcome the drawbacks presented by the previous technologies, such as the continuous operation (measuring and data transmission) for a wide current range conducted in the medium voltage transmission lines, damage due to lightning strikes, accuracy dependency on vibration, position and temperatures. The LPIT attends the accuracy criteria of IEC 61869-10 and IEC 61869-11 in terms of current and voltage accuracy and it attends the practical criteria adopted by Utilities companies including voltage measurements without removing the coating of the covered conductors.

INTRA- AND INTER-RATER RELIABILITY OF A BIOPHOTOGRAMMETRIC ASSESSMENT PROTOCOL FOR PRETERM INFANTS.

Objective: To measure the intra- and inter-rater reliability of a biophotogrammetric assessment protocol for thoracoabdominal motion in preterm infants.

Methods: This is an analytical cross-sectional study. Footage of 40 preterm infants was made in two views (lateral and anterior).

Personality and psychopathology heterogeneity in MMPI-2 and health-related features in fibromyalgia patients.

Personality and psychopathology features may play an important role as predisposing factors for fibromyalgia (FM). However, psychological heterogeneity of FM patients has been suggested. Based on two personality psychopathology clusters, we intend to explore psychological heterogeneity in FM patients, specifically, to identify if personality features had other psychological and psychopathological correlates.

Temporal assessment of neonatal pain after airway aspiration.

Objective: To temporally assess a painful stimulus in premature infants using 3 neonatal pain scales.

Methods: A total of 83 premature infants were observed during airway aspiration by 3 evaluators (E1, E2 and E3) using 3 pain assessment scales (Neonatal Facial Coding System - NFCS; Neonatal Infant Pain Scale - NIPS; and Premature Infant Pain Profile - PIPP) at 5 time points: T1 (before airway aspiration), T2 (during airway aspiration), T3 (1 minute after airway aspiration), T4 (3 minutes after airway aspiration), and T5 (5 minutes after airway aspiration). Light's Kappa (agreement among examiners and among scales at each time point) and the McNemar test (comparison among time points) were used considering p < 0.

NEONATAL PAIN: CHARACTERIZATION OF THE PHYSIOTHERAPIST'S PERCEPTION IN THE NEONATAL INTENSIVE CARE UNIT.

Objective: To describe the perception of physiotherapists in neonatal units regarding pain, the use of measurement scales and strategies that minimize pain.

Methods: Interviews were conducted with physiotherapists in hospitals with neonatal units between 2013 and 2015 in Rio de Janeiro. The questions concerned the knowledge of the feeling of pain, from its recognition to its care or treatment.

Static and Dynamic Evaluation of a Winding Deformation FBG Sensor for Power Transformer Applications.

Power transformer is the most important and expensive equipment used in the electric power industry. Fiber Bragg grating (FBG) sensors has stood out as a flexible and particularly suitable tool for power transformer monitoring being a passive and dielectric sensor element. In this work we evaluated the performance of FBG pressure sensors developed to monitor the static and dynamic pressure in high voltage winding transformers during events such as short-circuit and inrush current.

Maturation-associated gene expression profiles during normal human bone marrow erythropoiesis.

Erythropoiesis has been extensively studied using in vitro and in vivo animal models. Despite this, there is still limited data about the gene expression profiles (GEP) of primary (ex vivo) normal human bone marrow (BM) erythroid maturation. We investigated the GEP of nucleated red blood cell (NRBC) precursors during normal human BM erythropoiesis.

Maturation-associated gene expression profiles along normal human bone marrow monopoiesis.

Human monopoiesis is a tightly coordinated process which starts in the bone marrow (BM) haematopoietic stem cell (HSC) compartment and leads to the production of circulating blood mature monocytes. Although mature monocytes/macrophages have been extensively studied in both normal or inflammatory conditions, monopoiesis has only been assessed in vitro and in vivo animal models, due to low frequency of the monocytic precursors in the normal human BM. Here we investigated the transcriptional profile along normal human BM monopoiesis.

Antifungal activity of extracts from Piper aduncum leaves prepared by different solvents and extraction techniques against dermatophytes Trichophyton rubrum and Trichophyton interdigitale.

The effects of different solvents and extraction techniques upon the phytochemical profile and anti-Trichophyton activity of extracts from Piper aduncum leaves were evaluated. Extract done by maceration method with ethanol has higher content of sesquiterpenes and antifungal activity. This extract may be useful as an alternative treatment for dermatophytosis.

Harmonization of light scatter and fluorescence flow cytometry profiles obtained after staining peripheral blood leucocytes for cell surface-only versus intracellular antigens with the Fix & Perm reagent.

Staining for intracellular markers with the Fix & Perm reagent is associated with variations in the scatter properties of leucocytes, limiting automated analysis of flow cytometry (FCM) data. Here, we investigated those variables significantly contributing to changes in the light scatter, autofluorescence, and bcl2 staining characteristics of peripheral blood (PB) leucocytes, after fixation with Fix & Perm. Our major aim was to evaluate a new mathematical approach for automated harmonization of FCM data from datafiles corresponding to aliquots of a sample treated with cell-surface-only versus Fix & Perm intracellular staining techniques.

Breast cancer, poverty and mental health: emotional response to the disease in women from popular classes.

This study aimed to analyze the emotional response of a low-income group of women to the breast cancer. The sample was composed by 15 patients from a mastectomized women's support entity. Data were collected through individual face-to-face semi-structured interview.

Ultrasound promoted synthesis of 5-hydroxy-5-trihalomethyl-4,5-dihydroisoxazoles and beta-enamino trihalomethyl ketones in water.

A convenient method for the preparation of 5-hydroxy-5-trihalo-4,5-dihydroisoxazoles and beta-enamino trihalomethyl ketones, from the reaction of 1,1,1-trihalo-4-alkoxy-3-alken-2-ones with hydroxylamine and anilines, respectively, using water as solvent and under ultrasound irradiation is reported.