Ultrasonographic anatomy of the distal flexor structures of the thoracic members of rodeo bulls.

The veterinarian is responsible for monitoring and inspecting rodeos, thus ensuring the welfare of the animals. Clinical follow-up is of great importance in animal athletes, and ultrasound has become an important, widely used complementary diagnostic method in the evaluation of these animals. In equine medicine and dairy cattle, there are several studies that describe tendon injuries through ultrasonography.

Animal Model of Video-Assisted Cecum and Ileum Instrumentation for Equine Visceral Pain Study.

This study aimed to describe an animal model for studying equine visceral pain using minimally invasive and video-assisted cecum and ileum instrumentation. The access to the cecum and ileum was affected because of a previous typhlostomy. For video-assisted distention of the cecum and ileum, a distention device, which we developed using an endotracheal Rusch probe, was used, adapted, and coupled to a cuffometer to inflate and measure the pressure of the cuff attached to its distal portion.

Sex identification of the ornamental amazon fish Astronotus ocellatus by videoceloscopy and gonadal biopsy.

This study was conducted to evaluate and validate the efficacy and safety of videoceloscopy and gonadal biopsy as sexing methods for the A. ocellatus. A total of 31 adult individuals were used.

Fecal specimens preparation methods for PCR diagnosis of human taeniosis.

Sample preparation and DNA extraction protocols for DNA amplification by PCR, which can be applied in human fecal samples for taeniasis diagnosis, are described. DNA extracted from fecal specimens with phenol/chloroform/isoamilic alcohol and DNAzol reagent had to be first purified to generate fragments of 170 pb and 600 pb by HDP2-PCR. This purification step was not necessary with the use of QIAmp DNA stool mini kit.

Taenia saginata: polymerase chain reaction for taeniasis diagnosis in human fecal samples.

The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads.