Publications by authors named "Rodolfo Daetz"
Article Synopsis
- Metritis is a common inflammatory uterine disease in dairy cows, affecting about 20% after giving birth, and is linked to certain bacteria such as Fusobacterium, Bacteroides, and Porphyromonas.
- In a study, ceftiofur was given to some cows, leading to significant changes in the uterine microbiota, notably a decrease in Fusobacterium and an increase in certain biosynthesis pathways.
- Although ceftiofur reduced temperature and total bacteria in the uterus, other bacteria like Bacteroides and Porphyromonas remained unaffected, indicating a targeted impact on the microbial community in metritis cases.
Effect of Chitosan Microparticles on the Uterine Microbiome of Dairy Cows with Metritis.
Appl Environ Microbiol
September 2020
The objective of this study was to evaluate the effect of chitosan microparticles on the uterine microbiome of cows with metritis. Dairy cows with metritis ( = 89) were assigned to 1 of 3 treatments: chitosan microparticles ( = 21), in which the cows received an intrauterine infusion of chitosan microparticles at metritis diagnosis (day 0), day 2, and day 4; ceftiofur ( = 25), in which the cows received a subcutaneous injection of ceftiofur on day 0 and day 3; and no intrauterine or subcutaneous treatment ( = 23). Nonmetritic cows ( = 20) were healthy cows matched with cows with metritis by the number of days postpartum at metritis diagnosis.
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- Metritis in dairy cows is primarily caused by a polymicrobial infection, with new research questioning the significance of well-known pathogens like Escherichia coli and Trueperella pyogenes, while highlighting emerging pathogens such as Bacteroides pyogenes, Porphyromonas levii, and Helcococcus ovis.
- In a study involving 39 cows, researchers classified them into three groups—Healthy, Metritis without fever (MNoFever), and Metritis with fever (MFever)—and measured both bacterial levels and rectal temperature.
- Results showed that both MNoFever and MFever cows had higher total bacterial counts and specific pathogens compared to Healthy cows, confirming the role of certain bacteria in
Objective: This study aimed to evaluate bacterial and host factors causing a fever in cows with metritis. For that, we investigated uterine microbiota using a metagenomic sequencing of the 16S rRNA gene (Study 1), and immune response parameters (Study 2) in metritic cows with and without a fever.
Principal Findings (study1): Bacterial communities were similar between the MNoFever and MFever groups based on distance metrics of relative abundance of bacteria.
The objective of this study was to evaluate the progression of the uterine microbiota from calving until establishment of metritis. Uterine swabs (n = 72) collected at 0, 2, and 6 ± 2 days postpartum (dpp) from 12 metritic and 12 healthy cows were used for metagenomic sequencing of the 16S rRNA gene on the Illumina MiSeq platform. A heat map showed that uterine microbiota was established at calving.
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