Pluripotent Nontumorigenic Adipose Tissue-Derived Muse Cells have Immunomodulatory Capacity Mediated by Transforming Growth Factor-β1.

Adult mesenchymal stromal cell-based interventions have shown promising results in a broad range of diseases. However, their use has faced limited effectiveness owing to the low survival rates and susceptibility to environmental stress on transplantation. We describe the cellular and molecular characteristics of multilineage-differentiating stress-enduring (Muse) cells derived from adipose tissue (AT), a subpopulation of pluripotent stem cells isolated from human lipoaspirates.

The Effects of Fat Harvesting and Preparation, Air Exposure, Obesity, and Stem Cell Enrichment on Adipocyte Viability Prior to Graft Transplantation.

Background: Adipocyte viability is affected by fat preparation and processing methods, but rigorous and objective studies of these relationships are lacking.

Objectives: The authors conducted a comprehensive evaluation of variables affecting adipocyte viability prior to injection of fat at the recipient site.

Methods: Lipoaspirates from 48 patients were processed by high or low vacuum pressure, decantation, electric or manual centrifugation, concentration with cotton gauze, washing, repeated syringe transfer, exposure to lidocaine, and exposure to air.