Growth factors in the regulation of reparative response in the presence of peritoneal damage.

Objectives: To study the expression of growth factors in the regulation of tissue repair after peritoneal damage tissue response to peritoneal damage.

Methods: Experimental study in 35 male Wistar rats determining the evolution over time of the tissue response to aseptic peritoneal damage. A standardized bowel and peritoneal lesions were created in the right lower quadrant by laparotomy.

Protective Ventilation Improves Gas Exchange, Reduces Incidence of Atelectases, and Affects Metabolic Response in Major Pancreatoduodenal Surgery.

Background: Protective perioperative ventilation has been shown to improve outcomes and reduce the incidence of postoperative pulmonary complications. The goal of this study was to assess the effects of ventilation with low tidal volume (V) either alone or in a combination with moderate permissive hypercapnia in major pancreatoduodenal interventions.

Materials And Methods: Sixty adult patients scheduled for elective pancreatoduodenal surgery with duration >2 h were enrolled into a prospective single-center study.

POSTOPERATIVE PULMONARY COMPLICATIONS AND ACUTE RESPIRATORY DISTRESS SYNDROME -BETTER PREVENT THEN TREAT.

In parallel with increasing number, duration and extensiveness of surgical interventions, postoperative pulmonary complications (PPC) and acute respiratory distress syndrome (ARDS) remain the major challenges for anesthesiologists and surgical ICU physicians. PPC and ARDS have multiple risk factors that should be recognized early and modifed within the appropriate "time window ". Today we possess reliable models (ARISCAT LIPS, EALI etc.

Investigation of the noncovalent interactions between anti-amyloid agents and amyloid beta peptides by ESI-MS.

This paper describes an efficient and reproducible screening method for identifying low molecular weight compounds that bind to amyloid beta peptides (Abeta) peptides using electrospray ionization mass spectrometry (ESI-MS). Low molecular weight compounds capable of interacting with soluble Abeta may be able to modulate/inhibit the Abeta aggregation process and serve as potential disease-modifying agents for AD. The present approach was used to rank the binding affinity of a library of compounds to Abeta1-40 peptide.

Use of recombinant interleukin-2 for intrapleural therapy of tumor-associated pleurisy.

Clinical efficiency of low dose Roncoleukin was studied in 30 patients with metastatic exudative pleurisy. Intrapleural therapy proved to be highly effective (overall effect reached 84%), was well tolerated, and improved patients' quality of life.

[Biochemical markers of the osteomyelitis development].

The authors describe a procedure for early and cost-effective prediction of the development of traumatic osteomyelitis in patients with bone injury via the biochemical study of a patient's blood, which determines the activity of acid phosphatase and the concentration of Mg, Ca, Cl, Cu, Zn, and thyroid-stimulating hormone, followed by calculations using the medians of regional values of the study parameters.

[Serum levels of sexual hormones in young and middle-age healthy subjects with risk factors of coronary heart disease and patients with coronary heart disease].

Aim: To study sexual hormones levels in risk of beginning and development of coronary heart disease (CHD) in young and middle-aged (up to 50 years) men and women.

Material And Methods: Sexual hormones were studied in 105 patients (53 males and 52 females) aged 25-50 years. Of them, 88 suffered from IHD and 17 had CHD risk factors.

Preparation and reactivity of aminoacyl pyroglutamates. Facile synthesis of 10-membered-ring cyclic dipeptides derived from 1,4-diaminobutyric and glutamic acids.

A number of protected proline-containing dipeptides Boc-Xaa-Pro-OBu(t) were converted via epimerization-free oxidation with RuO4 to dipeptides with an internal pyroglutamic acid residue, Boc-Xaa-Glp-OBu(t). The latter were subjected to oxidative Hoffman-type rearrangement induced by PhI[OC(O)CF3]2 to give N-(aminoacyl)-pyroglutamates. The behavior of these derivatives under basic conditions was studied, and for two such a derivatives an aminoacyl incorporation reaction was observed, producing otherwise poorly accessible 10-membered-ring dilactams derived from 1,4-diaminobutyric and glutamic acids in practicable yields.

[Formalized cytological report in oncology].

A formalised report (FR) offers a set of standard phrases to be used to state (1) whether findings of a cytologic study contain new information; (2) the degree of certainty in understanding the significance of morphological forms of available international classifications of a pathology said morphological evidence is compatible with. The use of that FR provides a means of evaluating reported cytologic conclusions, and comparing them with relevant postmortem diagnosis. This makes it possible to ascertain with greater or less certainty, with which nosological forms a doctor deals.

[Total solid phase synthesis of gamma-subunit of cGMP phosphodiesterase from bovine retina and physicochemical properties of synthetic protein].

The 87-membered polypeptide with the sequence of the gamma subunit of cGMP phosphodiesterase from bovine retina rods (PDE gamma) was synthesized by the solid phase method. Two synthetic approaches, which were based on the Boc/Bzl-strategy, were used; both syntheses were carried out in a continuous-flow reactor with swellographic monitoring. In the first approach, five Arg residues were coupled in the form of Boc-Arg(Z)2-OH and the final cleavage of the peptide from the support was effected by the mixture of CF3SO2SiMe3 and thionisole in trifluoroacetic acid.

[Conformational states of cyclic dipeptides with lactam bond between omega-functions of lysine and aspartic or glutamic acid residues].

The conformations of H-Lys-Asp-OH and H-Glu-Lys-OH cyclic dipeptides were subjected to theoretical analysis by the method of atom-atom potentials with flexible geometry. Constants of spin-spin coupling of vicinal protons were calculated for the theoretical conformers of both dipeptides. CD and NMR spectra were measured for both peptides synthesized, and the calculated and experimental values of spin-spin coupling constants were compared.

Phyllodes tumour: cytologic and histologic presentation of 22 cases, and immunohistochemical demonstration of p53.

A retrospective study of 22 cases of phyllodes tumour (PT) was undertaken to evaluate the potential value of fine needle aspiration (FNA) cytology in the diagnosis of benign and borderline PT. Histological material was available from 12 patients with typical benign PT (group 1), six patients with less typical changes (group 2) and four cases of borderline PT (group 3). Cytological presentation of PT in these cases was similar to that described by other cytologists, although abundant cellular material was obtained in only eight FNAs, naked nuclei were present in nine cases only, and atypical or suspicious cytological features were found in seven cases.

[Permissible level of laser radiation of wavelength 10.6 microns].

In a four-month experiment with 102 male rabbits and 42 HRS/U mice and 30 days after termination thereof, study was made of local and general response of an organism to the effect of laser radiation (10.6 microns wave length and energy exposition 1/7 of the threshold). The radiation tolerance level was estimated.

[The stimulating effect of helium-neon laser radiation on rabbit eyes].

Local and general reactions have been studied after subjecting a rabbit eye to dispersed helium-neon laser radiation, 8 x 10(-6) wt/cm-2, 10 min daily for 10 days. There were recorded increase of blood filling of the uveal tract of the eye, changes in the indices of systemic hemodynamics, systolic and diastolic pressure, stroke and minute volume of blood volume, activation of separate links of antioxidant system connected both with oxidation-reduction of thiol disulfide system and the functioning of antiperoxide enzymes. The activation of antioxidant system has a local character, the changes in the retina of the eye and pigmented epithelium is expressed stronger than in the peripheral blood.

[Sensitivity of animals to CO2 laser radiation].

The study was undertaken to analyze the state of visual, cardiovascular and antioxidant systems along with peripheral blood exposed to laser emission with the wavelength of 10.6 mm and energetic exposure equal to that of threshold (21 J.cm2) and subthreshold (1/7 of the threshold value).

[The antioxidant system in noise-induced stress].

Some patterns of the antioxidant system were altered in rat blood and tissues after treatment with noise of 90 dB. Phase alterations were found in content of SH- and SS-groups, in ascorbate-dependent redox balance as well as in activity of glutathione reductase, glucose-6-phosphate- and lactate dehydrogenases. These data suggest that the antioxidant system is of importance in the adaptation reactions.

[The role of the antioxidant system in the mechanism of adaptation to intensive noise].

It has been shown in the experiment with albino rats that the prolonged influence of stable noise (90 dB) results in a shift of the redox equilibrium in the thiol-disulphide and ascorbate systems of the adrenal gland and myocardium tissues towards oxidation and the activity of glucoso-6-phosphate dehydrogenase and superoxide dismutase decreases. A concept on the pathogenetic role of the redox equilibrium disturbance in the thiol-disulphide system, in particular, in the development of noise pathology is suggested.

[Effect of unithiol and acetylcysteine on lipid peroxidation and the erythrocyte antioxidant system in sensitized guinea pigs].

The effect of unithiol and acetylcysteine on lipid peroxidation, thioldisulfide equilibrium, glucose-6-phosphate dehydrogenase, glutathione reductase and lactate dehydrogenase activity and on erythrocyte resistance was studied in guinea-pigs during sensitization with C. maltosa. Sensibilized animals receiving thiol antioxidants showed partial restoration of normal biochemical levels.